MATERIALS AND METHODS

Collection of cashew apples: The undamaged and fully ripened cashew apples were collected from the Ghandarvakottai, Pudukottai District, Tamil Nadu India in November, 2011. The apples were brought as fresh to the Food Microbiology Laboratory of Indian Institute of crop processing technology, Thanjavur, Tamil Nadu and immediately processed.

Extraction of fruit juice: Cashew apple juice was extracted from selected cashew apples by Fruit Juice Extractor (GaganUdyog, Jalandhar-4, India) available at Incubation Centre, IICPT, Thanjavur, Tamil Nadu, India.

Minerals analysis using ICP-OES: For analysis of minerals 100 mL of the fruit juice was ashed using Muffle furnace for 4 h and it was cooled for 30 mts. After cooling the ash was dissolved with 20 mL of 1:1 ratio HCl:distilled water. The volume of the solution was reduced to 5-8 mL by evaporating in water bath and it was made up to 50 mL. The sample was used for determination of minerals using inductively Coupled Plasma Optical Emission Spectrophotometer (PerkinElmer). Cashew apple juice was extracted with chloroform and that extract was analyzed using GC-MS for different bioactive components.

RESULTS AND DISCUSSION

The cashew apple juice was found to contain a total of nine minerals in different level. Minerals like magnesium, sodium, iron, calcium, copper, sodium and zinc were also present at significant level of 279.70, 204, 133.9, 80.42, 1.17, 204.0 and 16.48 ppm, respectively in cashew apple juice (Table 1). Among the various minerals potassium was observed in high level (3337 ppm) followed by phosphorous (440.20 ppm). Potassium (K) is very essential element to prevent bone demineralization which is by controlling of calcium loss in urine (Tucker et al., 1999; He and MacGregor, 2001).

Table 1:	Mineral content in cashew apple juice

Nagaraja (2009) also reported that some varieties of cashew apple are rich in potassium.

Copper is an essential and beneficial element in human metabolism and the average daily dietary requirement for copper in the adult human has been estimated as 2 mg and for infants and children at 0.05 mg kg^-1 b.wt. (Browning, 1969; WHO, 2004). The NRC (1980) reported that safe and adequate daily dietary intakes of copper ranging from 0.5-0.7 mg day^-1 for infants of 6 months age or less up to 2-3 mg day^-1 for adults. The copper content of 1.07 ppm observed in the cashew apple juice is within the safe prescribed limits of infants.

Sodium is the principle extracellular cation and is used for osmoregulation in inter modular fluid of human body. The recommended daily allowance of sodium is 115-75000 ppm for infants, 324-975 mg kg^-1 for children and 1100-3300 ppm for adults (Crook, 2006). Enzymes are involved in macronutrient metabolism and cell replications are mainly Zn dependent (Hays and Swenson, 1985; Arinola, 2008). Zinc is widely distributed in plant and animal tissues and present in all living cells. In cashew apple juice 16.48 ppm of Zinc was observed.

Magnesium is an active element in several enzyme systems in which thymine pyrophosphate is a cofactor. Oxidative phosphorylation is greatly reduced in the absence of magnesium. It also activates pyruvic acid carboxylase, pyruvic acid oxidase and the condensing enzyme for the reactions in the citric acid cycle (Murray et al., 2000). Even though the value obtained in the cashew apple juice is low as compared to the standard value, it can be used as natural sources of minerals with other food and beverages to rectify the child malnutrition.

Activity of phytocomponents identified in chloroform extract of cashew apple by GC-MS: The studies on the active principles in the chloroform extract of cashew apple by GC-MS analysis clearly showed the presence of twelve compounds (Table 2). The compounds were identified as 9,12-octadecadienoic acid (Z,Z)-15-hydroxy-pentadecanoic acid, 15-hydroxy-pentadecanoic acid and 9,12-octadecadienoyl chloride (Z,Z)- etc. Among the various compounds 9,12-octadecadienoic acid (Z,Z)- was found to be the highest peak area (50.16%) followed by 9,12-octadecadienoyl chloride (Z,Z)- (21.35%) and 15-hydroxy-pentadecanoic acid (16.32%). Unsaturated fatty acids exist in the cashew apple extract, especially polyunsaturated fatty acids have a wide range of biological functions, especially 9,12-octadecadienoic acid which belongs to the family of n-6 polyunsaturated fatty acids and (Z, Z, Z)-9,12,15-octadecatrienoic acid which belongs to n-3 family of polyunsaturated fatty acids (Zhang et al., 2006), plays an important role in human and animal body.

Table 2:	Bioactive compounds identified in the chloroform extract of the cashew apple by GC-MS

In many cases, the two families of polyunsaturated fatty acids in the functional constraints on the co-ordination, co regulating organism’s life activity, include lipid metabolism, cardiovascular function regulation, blood glucose concentration regulation, cell regulation, immune regulation and other aspects. Among the various components 9,12-octadecadienoic acid (Z, Z)- is found to be the maximum peak area in cashew apple juice (50.16%). The results presented herein are in agreement with those presented by Garruti et al. (2003) who evaluated volatile substances consisting of esters and aldehydes in cashew apple. Like that of our present study chemical composition of the cashew apple has been the subject of investigation by number of authors (Kubo et al., 2006).

CONCLUSION

The cashew apple shows varying quantity of minerals and among them potassium is found to be highest amount of 3337.0 ppm followed by phosphorous, magnesium and sodium. Among the bioactive compounds found in extract of cashew apple 9,12-octadecadienoic acid (Z,Z)- is found to be higher quantity. Hence the biowaste of cashew apple can be act as source of antioxidant and mineral supplement in food as allied product development.

ACKNOWLEDGMENT

The authors very much thank Dr. K. Alagusundaram, Director, Indian Institute of Crop Processing Technology, Thanjavur for providing all the facilities and support to carry out the work.