Prey-predator Interaction and Info-chemical Behavior of Rhynocoris fuscipes (Fab.) on Three Agricultural Pests (Heteroptera: Reduviidae)
Diversity, ubiquity of anti-predator adaptations has an important direct effect of info-chemical based phenomenon of prey and predator as well as prey density. This work explained three agricultural pests having info-chemical cues also influence the predatory efficiency of the reduviid predator of Rhynocoris fuscipes (Fab.). In this context, the aim of this study was to identify the prey-predator interaction with specific chemical cues on biological control agent of Rhynocoris fuscipes treated with three agriculturally important pests. The polyphagous reduviid predator Rhynocoris fuscipes (Fab.) (Heteroptera: Reduviidae) is a potential biocontrol agent for the red cotton bug Dysdercus cingulatus (Fabricius), tobacco caterpillar, Spodoptera litura (Fab.) Lepidoptera: Noctuiidae) and castor pest Achaea janata (Linn.). The influence of info-chemicals from these three species of groundnut pests on the prey-searching and probing behaviour of the reduviid predator was studied. The water fraction of the mixture of chloroform and methanol extract of A. janata elicited maximum response in R. fuscipes when compared with S. litura and D. cingulatus. Adults and fifth instars were consumed more time with A. janata extracts as compared to other instars. Minimum and maximum response was observed in case of S. litura and D. cingulatus extract. Among the five life stages fifth and adult stages of the R. fuscipes predators preferred fifth instar of A. janata, S. litura where as fourth and fifth nymphal instars were quickly preferred third to fifth instars of D. cingulatus. These findings could be used in the application of R. fuscipes as a biocontrol agent in the management of cotton, castor and groundnut pests.
Received: September 28, 2011;
Accepted: November 03, 2011;
Published: December 12, 2011
Rhynocoris fuscipes is a polyphagous predator present in India. It is
an effective biological control agent of cotton and groundnut pests (Ambrose
and Claver, 1995, 1997; George
et al., 2000; Prasanna and Shirely, 2002).
Semilooper, Achea janata (Linn.) is a key insect pest of castor, Ricinus
communis. The larvae cause extensive defoliation during July-September and
also feed on tender capsules Prasad et al. (2007).
Another pest of Dysdercus cingulatus (Fab.) (Hemiptera: Pyrrhocoridae)
is a serious pest of cotton which distributed in all the cotton growing regions
in India. The chemicals, which govern the prey and predator sinteraction are
generally called info chemicals (Nordlund et al., 1981;
Ambrose and Claver, 1999). It includes kairomones and
allomones, which influence the prey-predator interaction (Ananathakrishnan,
2002). The chemical cues emanating from the pests act as arrestants or stimulants
to the predators and increase their search for the prey (Singh
et al., 2002). According to Duffey (1980),
kairomones and allomones are especially present in the nutrient storage sites
like gut, heamolymph and fat body. Many works have been carried out to find
out the role of kairomones from hemipteran pests in bring forth (Barbosa
and Saunders, 1985) and lepidopteron pests (Bakthavatsalem
et al., 2000; Singh et al., 2002)
in eliciting a host searching behaviour of many generalist predators. In reduviids,
the chemical-based prey-predator interaction was studied by Sahayaraj
and Paulraj (2001) and Sahayaraj and Delma (2004).
Recently, Cokl and Millar (2009) published the prey
predators signals emitted as modification of the physical and chemical environment
and are translated by the receivers (predators) to provide information about
such factors as the kairomonal expression and based upon the physiological or
behavioural state. Currently, Deligeorgidis et al.
(2011) studied modeling of Predatory Effect of Coccinellidae with various
pests. Eventhough most of the above studies were carried out with organic solvent
extract of any pests, very few work was done on water extracts. Hence, the present
study aimed to investigate the behaviour of R. marginatus on the fractions
of the combination of both chloroform with methanol extracts of D. cingulatus
(Fab.), Spodoptera litura (Fab.) and Achea janata (Linn.). So,
that the info-chemicals present in the water fraction, that elicit the host
seeking response in R. fuscipes can be identified.
MATERIALS AND METHODS
This research was conducted from July 15-2009 to January 27-2010. R. fuscipes
was collected from the groundnut ecosystem and in and around the Scott Christian
College, Nagercoil and Kanyakumari, India. Then collected various stages of
this predators were maintained on Corcyra cephalonica (Stainton) larvae
under laboratory conditions (30±1.5°C, L13:D11 Photoperiod and relative
humidity 73±4%) using the method developed by Sahayaraj
(2002). Different life stages of S. litura and A. janata then
D. cingulatus were collected from the Erachakulam, then reared on groundnut,
castor leaves and cotton leaves respectively under the same laboratory conditions.
Laboratory emerged fourth instar larvae of S. litura and Achaea janata
and adults of D. cingulatus (both male and female) were used for this
Extracts of the info chemicals from the experimental pests: The specific
info chemicals emitting from D. cingulatus, S. litura and A.
janata were extracted using mixture of chloroform and methanol (Singh
et al., 2002). Twenty to thirty-five adults were weighed and placed
in a stopper bottle to which 75 mL of chloroform and methanol (1:1) was added.
Then the bottle was shaken at room temperature for two hours and kept at 60°C
in water bath for 20 min. After that the extract was fractionated in to mixture
of chloroform, methanol and water soluble fractions then the latter fractions
also been used for this experiment. The same procedure was followed for S.
litura and A. janata.
Behavioural Bio-assay: Five armed glass olfactometer designed by Sahayaraj
and Paulraj (2001), was used to study the feeding behaviour and consuming
time was calculated by the method of Cohen (2000) of
Statistical analysis: Correlation was observed between the approaching
behaviour and handling time for each pest separately (both control). One-way
ANOVA test were performed to determine the differences in the handling time
between the controls (water) and test (water fraction of the pest mixture of
chloroform and methanol extracts).
When the predators were introduced the central place of the olfactometer, R. fuscipes move towards odour source present in the Whatman paper with antenna facing direction mainly depends upon the needs of the each life stages of R. fuscipes. After getting a perfect orientation position, the reduviid palpated its antennae, then aroused and subsequently showed the other behavioural responses. Once the predators entered the test chamber, they exhibited exploration and probing behaviour by showing head lifting, approaching, rostrum cleaning and antenna cleaning.
R. marginatus showed maximum positive response on H. armigera as compared to that of D cingulatus and S. litura (Table 1). Based upon this respect to A. janata extract shows, 60, 70, 80 and 75% of 2, 3, 4 and 5 instars of R. marginatus showed positive response, respectively. The preference of R. fuscipes decreased for D. cingulatus (20, 40, 35 and 20 of 2, 3, 4 and 5 instars, respectively) and further, decreased response was observed with S. litura extract (10% for 2 and 5 instars). In chi square test both the II and V instar results were significant for A. janata and only fifth instar nymphs showed significant response of S. litura extract, where as in D. cingulatus 1, 2, 2 instar showed a significant results at 5% level (Table 1).
The feeding behaviour of R. marginatus (in terms of consuming time)
on three pest extracts is shown in Table 2. The second instar
R. fuscipes exhibited maximum handling time in A. janata extract
(12.28±2.49 min) followed by S. litura (8.54±0.45 min)
finally fifth instar of R. fuscipes consumed the pest of D. cingulatus
at 1.49±0.18 min. When compare with these three experimental pests. Among
these pests A. janata showed more consuming time than other two pests.
However, second and third instar nymphs of R. fuscipes showed no response
on D. cingulatus extract. The third instar nymphs spent more time with
The 4 and 5 nymphal showed maximum handling (10.31±2.40 and 11.46±1.57
min, respectively). From this study explained the consuming time was positive
correlate with the instars of the R. fuscipes predators.
|| Approaching behaviour of R. fuscipes to water soluble
fraction of three pests
|*Shows the significant at p<0.005% level
|| Consuming time (in min) of R. fuscipes (N±SE)
to the water fractions of the pests
One way ANOVA and Student-test were performed. The tests showed there is no
significant observation been made except between the fifth instars treated with
water fraction of the pests extracts and control.
The notable findings of the present study were as follows, the predatory behavioural
pattern of reduviid is arousal approach-rostral probing-injecting toxic saliva
and it can help to paralyzing-sucking post predatory behaviour (Ambrose
and Claver, 1995). The reduviids oriented towards the prey with facing antenna
after getting a perfect orientation position, the reduviid palpated its antennae,
then aroused and subsequently showed the other behavioral responses studied
by Ambrose (1999). Similar info chemical based predatory
behaviour related studies with other three economically important pests by the
following authors Sahayaraj and Paulraj (2001), Jhansilakshmi
et al. (2000) and Demirel (2007) ascribed
in other predatory insects. When we compare the approaching behaviour with handling
time by correlation, the results were significant (p<0.05). It was suggested
that the predators identify the volatile chemicals emitted by the prey body.
The volatiles emotions by the feaces of the pests also act as attractants in
prey-predator interactions (Nassar and Abdulah, 2005).
The presence of such volatiles in the feaces may be attributed to the digestion
of the plant products by the pests and other processes involving micro-organisms
present in the feaces (Ambrose and Claver, 1995; Maran,
1999). It has been reported that there are instar or stage specific blend
of chemicals emitted by feces of second or fourth instar larvae of Pieris species
In the present study, the experimental pests treated with mixture of Chloroform
and methanol immediately exuded fecal matter which was immiscible in the solvent.
The immiscible solvent portion was fractionated with water and used for this
study. Hence, we presumed that the volatiles present in the feaces not only
act as attractants but also elicit an intense host searching behaviour. The
predatory behavioural assay clearly showed the rostrum protrusion and sucking
were not observed in 3, 4 instars against D. cingulatus extract followed
by A. janata extract failed to elicit such as host seeking behaviour
in R. fuscipes. Similar study was carried out by Usha
Rani and Wakamura (1993). The same findings also agreed by Sahayaraj
and Delma (2004) and Sahayaraj et al. (2007a,
b) such as pest releasing volatiles mediate searching
behavior at longer distances, not to mention of host insect volatiles or herbivore-derived
chemicals The present study shows that the kairomones of the pest extract on
the filter paper elicited by the common host seeking behaviour in R. fuscipes.
The variation in the behaviour of R. fuscipes towards the three experimental
pests might be due to the different volatile chemicals of the pests, as explained
by Ananathakrishnan (2002) and George
et al. (2004). Many researchers found out the volatile chemicals
promoting the behaviour of natural enemies like Rhynocoris predators
as well as the fact that the same chemicals affect the growth and survival of
herbivores indicate that manipulation of volatile profiles contribute significantly
in biocontrol strategies (Annadurai et al., 1990).
Previously detailed studies made by Devanand and Rani (2008)
certain plant extracts and other pest extract also employed sometimes predators
possessed antifeedance and toxicity in the lepidopteran pest species (Yasuda,
1997). Therefore, the behaviour and success of natural enemies undergo considerable
variations when the pest colonizes different host plant species in response
to different volatile compounds which modify the behavioural sequence of natural
enemies described by Nordlund et al. (1981). In
case of that explained that the behavioural changes could be ascribed to the
specificity of the predator to the prey (pest) species. The relationships between
infestation ratio and population development of Pink Bollworm adults was investigated
with the help of various info chemical based predatory behaviour reported by
Unlu and Ozturk (2007).
From this study concludes when the three agricultural pests having info-chemical cues also influence the predatory efficiency of the reduviid predator of Rhynocoris fuscipes (Fab.). Normally predatory behaviour exhibited the exploration and probing behaviour by showing head lifting, approaching, rostrum cleaning and antenna cleaning based upon the unique nature of the preference of the reminiscent of pests. Among the life stages both fifth and adult stages of the R. fuscipes predators preferred fifth instar of A. janata, S. litura, where as fourth and fifth instar nymphs quickly preferred third to fifth instars of D. cingulatus. Finally, this research finding could be used in the application of reduviid predator such as R. fuscipes as a biocontrol agent in the management of cotton, castor and groundnut pests.
Authors were deeply gratitude to the College Correspondent Rev. Fr. Premkumar, MSW, Malankara Catholic College, Mariagiri for his strongly encouragement and his kind support for the preparation of this manuscript. The corresponding author would like to express the sincere thanks for Mr. M. Muthukumar (Research Scholar in CPRC, St. Xaviers College (Autonomous) Palayamkottai for his support and suggestion for this manuscript preparation.
1: Ambrose, D.P. and M.A. Claver, 1995. Functional response of Rhynocoris fuscipes (Fabricius) (Heteroptera: Reduviidae) to Riptortus clavatus Thunberg (Heteroptera: Alydidae). J. Biol. Control., 9: 74-77.
2: Ambrose, D.P. and M.A. Claver, 1997. Functional and numerical responses of the reduviid predator, Rhynocoris fuscipes F. (Heteroptera: Reduviidae) to cotton leaf worm Spodoptera litura F. (Lepidoptera: Noctuidae). J. Applied Entomol., 121: 331-336.
3: Annadurai, R.S., S. Murugesan, R. Senrayan, G. Guru Subramanian and T.N. Ananthakrishnan, 1990. Tritrophic Interactions in Heliothis armigera Hubner and its Natural Enemy Systems: A Chemical Ecology Approach (Noctuidae: Lepidopetra). In: Emerging Trends in Biological Control of Phytophagous Insects, Ananthakrishnan, T.N. (Ed.). Oxford and IBH Publishing Co., New Delhi, India, ISBN-13: 9788120406513, pp: 83-101
4: Ambrose, D.P., 1999. Assassin Bugs. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India, Pages: 337
5: Ambrose, D.P. and M.A. Claver, 1999. Suppresion of cotton leafworm Spodoptera litura, flower beetle Mylabris pustulata and red cotton bug Dysdercus cingulatus by Rhynocoris marginatus (Fab.) (Heteroptera: Reduviidae) in cotton field cages. J. Applied Entomol., 123: 225-229.
6: Ananathakrishnan, T.N., 2002. Molecular Messengers in insect Bioaccumulation: Modality and Revance in Biological Control. In: Insects Plants and Molecular Interactions, Ananathakrishnan, T.N. (Ed.). Madras Science Foundation, Chennai, India, pp: 53-58
7: Cokl, A.A. and J.G. Millar, 2009. Manipulation of insect signaling for monitoring and control of pest insects. Biorational Control Arthropod Pests, 2009: 279-316.
CrossRef | Direct Link |
8: Bakthavatsalem, N., S.P. Singh, P.L. Tandon, M. Chaudhary and S. Preethi, 2000. Synomone meiated behaviuoral responses of Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) to cotton infested by Helicoverpa armigera (Hubner) (Lepidoptera: Noctuiidae). J. Biol., 17: 50-54.
9: Barbosa, P. and J.A. Saunders, 1985. Plant Allelochemicals: Linkage Between Herbivores and their Natural Enemies. In: Chemically Mediated Interactions Between Plants and Other Organisms, Cooper-Driver, G.A., T. Swain and E.E. Conn (Eds.). Plenum Press, New York, USA., pp: 107-137
10: Cohen, A.C., 2000. Feeding fitness and quality of domesticated and feral predators: Effects of long-term rearing on artificial diet. Biol. Control, 17: 50-54.
11: Devanand, P. and P.U. Rani, 2008. Biological potency of certain plant extracts in management of two lepidopteran pests of Ricinus communis. J. Biopesticides, 1: 170-176.
Direct Link |
12: Duffey, S.S., 1980. Sequestration of plant naural compounds by insects. Annu. Rev. Entomol., 25: 447-477.
CrossRef | Direct Link |
13: George, P.J.E., M.A.Claver and D.P. Ambrose, 2000. Life table of R. fuscipes (Fab.) (Heteroptera: Reduviidae) reared on Corcyra cephalonica (Stainton). Pest Manage. Econ. Zool., 8: 57-63.
14: George, P.J.E., M.A. Claver and D.P. Ambrose, 2004. Insecticidal impacts on the differential and the total haemocyte counts in harpactorine reduviidea Rhynocoris fuscipes (Insecta: Heteroptera: Reduviidae). Hexapoda, 18: 49-55.
15: Jhansilakshmi, V., K. Krishniah, T. Linaiah and I.C. Pasalu, 2000. Rice leafhopper and planthopper honeydew as a source of host searching kairomone for the mired bug predator, Cyrtorhinus lividipennis (Reuter) (Hemiptera: Miridae). J. Biol. Control, 14: 7-13.
16: Prasanna, K.S. and D. Shirely, 2002. Influence of food quantity of the stadial period of Rhynocoris fuscipes (Fab.) (Heteroptera: Reduviidae). J. Entomol. Res., 26: 3-3.
17: Maran, S.P.M., 1999. Chosen reduviid predators-prey interaction, Nutritional and Kairamonal chemical ecology. Ph.D. Thesis, Manonmaniam Sundaranar University.
18: Nordlund, D.A., R.L. Jones and W.J. Lewis, 1981. Semiochemicals: Their Role in Pest Control. John Wiley, New York, USA
19: Prasad, Y.G., M. Prabhakar, A. Phanidhara and P.S. Vimala Devi, 2007. Development of Achea janata granulovirus as a biopesticide. Proceedings of the Biopesticidal International Conference, November, 2007, India, pp: 33-
20: Sahayaraj, K., 2002. Biopesticide an Indian scenario. Agribios, 1: 26-29.
21: Sahayaraj, K. and J.C.R. Delma, 2004. Chemically-mediated behaviour of the opredator Rhynocoris marginatus (Fab.) (Heteroptera: Reduvidae) searching insect prey. Belgiam J. Entomol., 6: 75-81.
22: Sahayaraj, K. and M.G. Paulraj, 2001. Behaviour of Rhynocoris marginatus (Fab.) to chemical cues from three-lepidopteron pest (Heteroptera: Reduviidae). J. Biol. control, 15: 1-14.
23: Singh, S., A.V.N. Paul, D. Prem and A.K. Singh, 2002. Kairomones of two host insects and their impacts on the egg parasitoids, Trichogramma brasiliensis (Ahmead) and T. exiguum Pinto, Platner and Oatman. Indian J. Entomol., 64: 96-106.
24: Deligeorgidis, N.P., C. Karypidis, P.N. Deligeorgidis, C.G. Ipsilandis, M. Vaiopoulou and G. Sidiropoulos, 2011. Modeling of predatory effect of coccinellidae. J. Entomol., 8: 73-80.
CrossRef | Direct Link |
25: Demirel, N., 2007. Infochemical pattern for true bugs. J. Entomol., 4: 267-274.
CrossRef | Direct Link |
26: Sahayaraj, K., P. Selvarj and R. Balasubramanian, 2007. Cell mediated immune response of Helicoverpa armigera hubner and Spodoptera litura fabricius to fern phytoecdysteron. J. Entomol., 4: 289-298.
CrossRef | Direct Link |
27: Sahayaraj, K., S.K. Sankaralinkam and R. Balasubramanian, 2007. Prey influence on the salivary gland and gut enzymes qualitative profile of Rhynocoris marginatus (Fab.) and Catamiarus brevipennis (Serville) (Heteropetera: Reduviidae). J. Entomol., 4: 331-336.
CrossRef | Direct Link |
28: Usha Rani, P. and S. Wakamura, 1993. Host acceptance behaviour of a predatory pentatomid Eocanthecona furcellata (Wolf) (Heteroptera: Pentotomidae) towards larvae of Spodoptera litura (Lepidoptera: Noctuiidae). Insect Sci. Applic., 14: 141-147.
29: Nassar, M.I. and A.M. Abdulah, 2005. Assessment of Boxus chinensis oil and precoceneii for the control of the red palm weevil, Rhynchophorus ferrugineus (Oliv.) (Coleoptera-Curculionidae) and the palm beetle Pseudophilus testaceous (Gahan) (Coleoptera-Cerambycidae). J. Entomol., 2: 1-8.
CrossRef | Direct Link |
30: Unlu, L. and I. Ozturk, 2007. Relationships between the numbers of adult male pink bollworm (Pectinophora gossypiella Saund.) catches on pheromone traps and infestation ratio of cotton bolls. J. Entomol., 4: 397-400.
CrossRef | Direct Link |
31: Yasuda, T., 1997. Chemicals cues from Spodoptera litura larvae elicit prey-locating behaviour by the predatory stink bug, Eocanthecona furcellata. Entomol. Exp. Appl., 82: 349-354.
Direct Link |