Influence of Blood Meal Type on Reproductive Performance of the Malaria Vector, Anopheles gambiae s.s. (Diptera: Culicidae)
This study elucidated the influence of blood meal type on reproductive performance of Anopheles gambiae s.s. Starting from three days old, An. gambiae mosquitoes, from a laboratory colony maintained following standard anopheline rearing techniques, were offered seven different blood meal types, through an artificial membrane. Thereafter, the mosquitoes were monitored for pre-larval and immature stage reproductive attributes. The results indicated that except for Embryony Period (EP) and Pupal Stage Duration (PSD), all attributes investigated varied significantly (p<0.05) among blood meals. While Blood Feeding Rate (BFR) was significantly highest and least in groups of mosquitoes fed with human (Mean = 94.69±3.36%) and pig (Mean = 48.54±10.13%) blood meals, respectively; the shortest Pre-oviposition Period (POP) were recorded among mosquitoes given human and cattle blood meals (Mean = 3.96±0.88 and 4.30±1.25 days, respectively). Also, human blood meal was the most ideal for Oviposition Rate (OR) (Mean = 79.20±12.39%) while chicken and pig blood meals were the least favourable (Mean = 46.80±9.55 and 48.05±6.34%, respectively). Fecundity (F) had similar distribution pattern as BFR while Egg Hatching Rate (HER) was generally low (<60%), irrespective of blood meal type. While Total Immature Stage Duration (TISD) varied widely among blood meal types, Total Larval Stage Duration (TLSD) was only significantly (p<0.05) longest in mosquitoes fed with pig blood meal (12.33±1.63 days). The epidemiological implications of these results were highlighted and discussed.
Received: February 28, 2011;
Accepted: April 19, 2011;
Published: May 30, 2011
Mosquito-borne diseases such as malaria, encephalitis, Rift Valley fever, dengue
fever, etc are a major public health threat in the tropics (Azari-Hamidian
et al., 2011). Malaria, for example, continues to ravage the tropical
and sub-tropical regions of the world without a close end in sight; such that
even under the most optimistic scenario, the disease may continue to be a major
public health threat in these regions for decades. According to USAID
(2005), malaria is endemic in over 100 countries where it threatens the
lives of over 3 billion people worldwide and is responsible for an estimated
900 million clinical cases of illness and almost 3 million deaths annually,
as well as, accounts for an estimated global loss of about 44.7 million Disability
Adjusted Life Years (DALYs). Sub-Saharan Africa is the worst hit with malaria
health and socio-economic burdens where about 90% of the worlds malaria-induced
deaths are recorded and is responsible for 20% of Africas infant mortality
and 10% overall disease burden (Roll-Back-Malaria, 2002;
WHO, 2003; Njan-Nloga et al.,
2007). Shah (2005) puts malaria-induced child mortality
rate in Africa at one every 30 sec.
The development of an effective vaccine for malaria has remained a mirage and
chemotherapy which proved very effective for more than three decades, is now
being rendered virtually ineffective in most parts of the world. The failure
of chemotherapy has been attributed to the emergence and rapid spread of drug-resistant
plasmodial parasites. This development has renewed stakeholders interest
in vector control, as the most viable strategy for reducing the present unacceptably
high burden of malaria in its areas of distribution (McCaffery
and Nauen, 2006). Effective malaria vector control however, requires a sound
knowledge of the biology, ecology and behaviour of the anopheline species involved
in transmission. In sub-Saharan Africa, the main malaria vectors are Anopheles
gambiae and An. funestus complexes (Kelly-Hope,
2009; Craig et al., 1999; Appawu
et al., 2004). Of these two anopheline complexes, the sibling species
An. gambiae s.s. has been adjudged the most efficient vector of human
malaria due to its highly anthropophilic, endophagic and endophilic tendencies,
as well as its ability to support the development of Plasmodium parasites
(Alavi et al., 2003). The vectorial capacity
of this mosquito is influenced by factors including ecological, climatic, behavioural,
etc. Though most of these factors have been well-studied (Bockarie
et al., 1994; Awolola et al., 2003;
Koenraadt et al., 2004; Olayemi
and Ande, 2008), published information directly relating the behavior of
mosquitoes, especially blood meal quality and quantity, to reproductive performance
are scanty (Ferdouse and Islam, 2005; Basseri
et al., 2010).
According to Briegel (1990), blood-feeding is pivotal
to a female mosquitos reproductive success, as most species require blood
meal to obtain amino acids from erythrocytes and plasma protein digestion to
synthesize yolk proteins for egg production (Hurd, 2003;
Roitberg and Gordon, 2005). Laboratory studies have
shown fecundity in mosquitoes to vary with source and size of the blood meal
with different blood meals resulting in significant variations in the number
of females that developed eggs and number of eggs per female (Taylor
and Hurd, 2001; Roitberg and Gordon, 2005). These
variations have been attributed to differences in proteins and their constituent
amino acids, in the blood meals (Prasad, 1987).
However, blood feeding may result in the ingestion of plasmodial parasites
which the mosquito can transmit during subsequent blood feeding. Thus, hosts
with superior blood quality for egg production may be preferred by anophelines
thereby increasing the frequency at which they feed on such hosts and hence,
malaria transmission. Burkot (1988) observed that the
prevalence of malaria in an area is greatly influenced by the process of host
selection by anopheline vectors which in turn, is influenced by many factors
including host preferences of the vectors, dictated by the quality of the hosts
blood. In Africa where An. gambiae complex is highly anthropophilic and
An. arabiensis is an opportunistic species feeding predominantly on man
but diverts to animals when the need arises, the latter species has significantly
lower sporozoite rates than the former (Taylor et al.,
1990; Githeko et al., 1993, 1994).
From the foregoing, it is clear that an investigation into the influence of
blood meal types on reproductive performance of An. gambiae s.s. is imperative,
especially, as the quantitative relationships are still poorly understood. These
information will, no doubts, shed more light on the evolution of blood meal
preferences in anophelines, as well as, the epidemiological implications of
such behaviour. This study was therefore, carried out to elucidate the influence
of conventional anopheline blood meal sources on pre-larval and immature stage
reproductive attributes of An. gambiae s.s.
MATERIALS AND METHODS
Source of mosquitoes and insectary environmental conditions: Day old
adult An. gambiae s.s. mosquitoes were obtained from a colony, in its
8th generation, maintained in the laboratory of the Department of Biological
Sciences, Federal University of Technology, Minna, Nigeria. The mosquitoes were
held in adult rearing cages (60x60x60), stocked at the rate of approximately
150 males and 150 females per cage and transferred to the insectary. Prio to
transfer to the insectary, the mosquitoes were maintained in a humid and cool
environment (El-Badry and Al-Ali, 2010). The Mean temperature
and relative humidity of the insectary, during the study period were 28.57±1.06°C
and 63.29±2.82%, respectively. The whole study was carried out and replicated
between the months of August 2008 and March 2009.
Mosquito rearing and maintenance: The mosquitoes were reared following
standard techniques (Das et al., 2007; Olayemi
and Ande, 2009). Blood meal administration were according to the techniques
of Lutomiah et al. (2007) and Moutailler
et al. (2007) though with slight modifications. Briefly, the blood
types were preserved by the addition of 0.3% Sodium Citrate and stored at 5°C
and later fed to the adult mosquitoes using a prophylactic membrane (Gold Circle).
The membranes were stretched and fastened to a 10 cm hood over a supporting
platform of netting material. The hood was then placed on top of the mosquito
cages and the preserved blood maintained at 40°C was poured into the membrane
for the mosquitoes to feed on, between the hours of 19:00 and 22:00. After each
blood meal feeding exercise, the female mosquitoes that successfully fed, evidenced
by the red-coloration engorgement of the abdomen were removed from the cages
and maintained individually in 20 mL plastic vials, with about 5 mL of distilled
water at the bottom to act as oviposition site. Also, similar number of male
mosquitoes, as those of the blood fed females were removed from the cages after
each feeding exercise, to ensure a constant sex ratio of 1:1 (Male: Female).
After oviposition, the eggs from the vials were transferred into plastic bowls where they were held for 12 to 72 h for hatching. Twenty four hours post-hatching, the larvae were transferred to larval rearing troughs (30x25x5 cm) half-filled with well water, at the rate of 50 larvae/trough. The larvae were fed with fish feed (Tetramin) at the rate of 0.32 mg/larva every other day when the water from the larval rearing troughs were changed carefully until pupation. The pupae were separated from the larvae daily and placed in plastic bowls (5 height and 20 cm diameter) half-filled with well water, at the rate of 50 pupae/bowl. The pupae plastic bowls were placed in adult-holding cages for imago emergence.
Investigation of reproductive performance: Blood Feeding Rate (BFR)
of the mosquitoes, defined as the proportion of female mosquitoes that successfully
blood-fed was determined as the proportion of female mosquitoes presented with
a blood meal type that successfully fed. Pre-Oviposition Period (POP) defined
as the interval preceding egg deposition, was estimated as the interval between
adult emergence and commencement of egg-laying. Oviposition Rate (OR) defined
as the proportion of blood-fed female mosquitoes that oviposited was determined
as the proportion of engorged mosquitoes that eventually laid eggs. Fecundity
(F) was determined as the total number of eggs laid per mosquito. Embryony Period
(EP), defined as egg incubation period was estimated as the interval between
oviposition and larval emergence. Egg Hatching Rate (EHR) was estimated from
the proportion of eggs hatched per mosquito.
Duration of the immature stages was determined according to the techniques
of Edillo et al. (2004) and Olayemi
and Ande (2009). Mean larval instar duration and pupal stage duration, in
days were estimated using the formula:
where, Di is duration of life stage, Ti is present Mean age and ti-1 is previous mean age at molting.
Statistical analyses: Statistical analyses for data collected were carried out using SPSS computer software for Windows, Version 15.00 (Install Shield Corporation, Inc.). One way Analysis of Variance (ANOVA) was done to calculate the significance level among means of reproductive attributes, due to blood meal types.
Pre-larval development reproductive performance: Table
1 shows the pre-larval development responses of An. gambiae s.s.
to different blood meal types. Blood Feeding Rate (BFR) by the mosquitoes varied
significantly (p<0.05) with the mosquitoes showing distinct preference for
human blood meal (Mean = 94.61±3.36%) while blood from pig was the least
favoured (Mean = 48.54±10.13%). The Pre-Oviposition Period (POP) was
significantly shorter in mosquitoes fed with human and cattle blood meals (Mean
= 3.96±0.88 and 4.30±1.25 days, respectively) but not significantly
(p>0.05) in individuals fed the other five types of blood meal (range = 5.13±1.77
to 6.82±2.00 days). Oviposition Rate (OR) also varied significantly (p<0.05)
across the blood meal types. As the case with the previous reproductive attributes,
OR was most favoured by human blood meal (Mean = 79.20±12.39%) although
this was not significantly different from the OR recorded for mosquitoes exposed
to dog blood meal. On the other hand, the groups of mosquitoes fed with chicken
and pig blood meals had the least number of individuals that oviposited (Mean
= 46.80±9.55 and 48.05±6.34%, respectively). As recorded for POP,
OR was not significantly different in the groups of mosquitoes given bovine
blood meals namely, cattle, goat and sheep meals laid the highest number of
eggs (Mean = 103.86±22.91 eggs/female) followed by mosquitoes (range
= 69.49±18.00 to 75.00±8.00%).
|| Influence of blood meal type on pre-larval reproductive performance
of Anopheles gambiae s.s
|Values followed by same superscript letter in a row are not
significantly different at p = 0.05 level of significance
|| Mean duration of immature life stages of Anopheles gambiae
s.s. in response to blood meal type
|Values followed by same superscript letter in a row are not
significantly different at p = 0.05 level of significance. All values were
measured in days
Fecundity (F) had a similar pattern of distribution across the blood meal types,
as that observed for BFR. Significantly, mosquitoes exposed to human blood fed
with cattle, goat and dog blood meals (range = 75.94±18.00 to 84.85±9.49
eggs/female) while mosquitoes given chicken, pig and sheep blood were the least
productive (range = 53.99±16.03 to 76.71±12.24 eggs/female). Embryony
Period (EP) had a distinct pattern of distribution across the blood meal types,
been the only pre-larval reproductive attribute that did not differ significantly
(p>0.05) among the blood meal types and where human blood meal was not the
best performing type; i.e., 1.54±0.60 days as against 1.25±0.39
days recorded for sheep blood meal type. Egg Hatching Rate (EHR) in the mosquitoes
was generally low (<60%) irrespective of blood meal type. However, OR was
significantly highest in mosquitoes fed with human and dog blood meals (Mean
= 56.90±13.48 and 52.62±4.80%, respectively) and lowest in mosquitoes
given cattle and chicken blood meals (Mean = 33.96±6.00 and 35.64±11.41%,
Immature stage duration: Table 2 shows the duration of immature life stages of An. gambiae s.s. fed with different blood meal types. Total Immature Stage Duration (TISD) varied significantly (p<0.05) across blood meal types, ranging from 10.76±2.32 days in mosquitoes given human blood meal to 14.12±2.26 days in those exposed to pig blood meal. Again, TISD elicited by the bovine blood meals were insignificantly different (p>0.05) and likewise those of human and dog blood meals. Pupal Stage Duration (PSD) was not significantly different among the blood meal types, ranging from 1.22±0.30 days in mosquitoes fed with human blood meal to 1.86±0.21 days in those given chicken blood meal. On the other hand, Total Larval Stage Duration (TLSD) was significantly longest in mosquitoes fed with pig blood meal (12.33±1.63 days) while those of the other blood meal types were insignificantly different (range = 9.54±2.02 days in human blood meal to 11.63±1.71 days in chicken blood meal). The distribution of the individual larval life stage duration across the blood meal types also varied significantly (p<0.05) with the L1 stage been the longest for most of the blood meal types.
The results of this study revealed that blood meal is indispensable for successful
reproduction in An. gambiae s.s., as none of the mosquitoes denied access
to blood meal oviposited. This result agrees with those of Takken
et al. (1998) and Klowden and Russell (2004)
and confirms that the species is truly an autogenous, i.e., depends on a blood
meal for the necessary nutrients to produce eggs. Also, the mosquito showed
distinct preferences for certain blood meal types, especially, human blood.
Similar results have been obtained elsewhere thus, resulting in the classification
of the species as pre-dominantly anthropophagic (Takken and
Lindsay, 2009). This feeding behavior stands An. gambiae s.s. in
good stead as a formidable vector of malaria and other human diseases such as
filariasis, as studies have shown that anopheline vectorial capacity is greatly
enhanced by the species propensity to feed on man (Basseri
et al., 2010). However, the fact that the mosquito fed, at least
fairly well, on all the other blood types presented to it, indicates plasticity
in its feeding behavior. Thus, it may be feasible to successfully divert the
species to blood sources other human, as aimed with the widespread distribution
of insecticide-treated bed nets with its attendant potential to reduce malaria
transmission. The Pre-oviposition Period in the mosquitoes responded commonly
to certain blood meal types, with blood meals from the larger mammals namely,
human and cattle, resulting in significantly shorter durations. This finding,
perhaps, explains why female anophline mosquitoes fly long distance in search
of blood meal and commonly breed and rest close to human habitations and cattle
sheds thus, having serious epidemiological implications.
The reproductive performance of An. gambiae s.s. was significantly influenced
by blood meal type. This finding agrees with previous reports which shows that
a strong positive correlation exists between blood meal type and mosquito reproductive
output, especially, fecundity and egg-hatching rates (Clements,
1992; Islam and Ferdousi, 1999). Oviposition Rate
(OR) was significantly highest in the groups of mosquitoes given human and dog
blood meals. This finding, perhaps, confirms the superiority of human blood
for oogenesis in mosquitoes. Though, the dog blood meal achieved, more or less,
a similar high level of OR as the human blood meal, the former may not enjoy
the same status as the latter, since it elicited significantly lower BFR and
longer POP in the mosquitoes. The similar influence of bovine blood meals on
OR may be due to the close evolutionary relationships among this group of mammals
(Vrba and Schaller, 2000) thus, resulting in the sharing
of identical blood quality. Fecundity (F) and BFR had more or less similar patterns
of distribution, an observation not entirely surprising. If mosquitoes could
discriminate among larval breeding habitats as a result of differential prospects
for immature developmental and survival rates (Edgerly et
al., 1998) they may as well possess innate ability to identify and select
blood meal sources with better potentials of supporting egg development and
ultimately higher fecundity.
Embryony Period (EP) was not significantly influenced by blood meal type. This
finding, perhaps, the conventional believe that blood meal is utilized by mosquitoes
primarily for egg development and maturation (Hurd, 2003;
Roitberg and Gordon, 2005). According to (WHO,
1975) embryony in mosquitoes is highly influenced by temperature; and this
is generally not a serious problem in the Tropics (Paaijmans
et al., 2010). The Egg Hatching Rate (EHR) recorded in this study
was generally low, the highest been 56.90±13.48% in the mosquitoes fed
with human blood. Earlier, Olayemi and Ande (2009) achieved
a higher EHR of 63.19±23.91% with the species, by simulating a crepuscular
dusk condition to encourage mating by the mosquitoes. However, despite the low
EHR, human and dog blood meals elicited significantly better performance in
this respect, perhaps, confirming the superiority of particularly human blood
meal for egg development in mosquitoes.
The results of this study equally revealed that larval stage developmental
rates of An. gambiae s.s. were significantly influenced by blood meal
types and their responses were similar to those of pre-larval reproductive attributes.
This finding indicates that while blood meal is of primary importance for egg
development in An. gambiae s.s., it never-the-less secondarily influences
immature stage developmental rates and hence, overall reproductive success in
the species. This, no doubts, will influence the vectorial capacity of the mosquito
with serious epidemiological consequences for malaria transmission.
The results of this study confirms that blood meal is inevitable for successful reproduction in An. gambiae s.s and the source significantly influences pre-larval and immature stage reproductive indices with human blood meal eliciting the highest level of productivity. This, perhaps, explains the dominance of anthropophagy among wild populations of An. gambiae s.s and perhaps, provides a justification for diverting the mosquito to blood meal sources other than man, especially with the use of insecticide-treated bed nets. While this strategy reduces malaria transmission in human populations by reducing vector-human host contacts, it ultimately may not reduce vector density, as the results of this study indicated that An. gambiae s.s. can, to some extent, reproduce successfully utilizing blood meal sources other than human hosts.
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