Cajanus Cajan, Zea mays and Vigna unguiculata are grains which are staple food crops for man and animals. They have high nutritive values and are used as weaning foods. These grains are harvested and ready for sale between the months of May and October. Excess harvests are stored to produce a food reserve as well as seed for planting (Udo et al., 2004). Stored grains are damaged when attacked by different species of insect leading to loss in weight and seed quality. The most devastating storage pests of maize is the maize weevil S. zeamais, while for pigeon pea and cowpea it is C. maculatus.
C. maculatus and S. zeamais are destructive pests and they cause serious management problem facing agriculture in developing countries. Attempts have been made to reduce their population or wipe them totally. Amongst the methods used are chemical methods- the use of insecticides. They have many advantages in the control of these pests. Their shortcomings ranged thus: High level of persistence in the environment, residual effect of synthetic, high maintain toxicity and pest resistance to mention just a few (Ashamo, 2004).
Many research works have been carried out to reduce the adverse effects of
storage pests. Local plant extracts, vegetable oils and other methods have been
employed to serve as alternatives to conventional chemical control (Oparaeke
et al., 1998; Odeyemi, 1998; Adedire and Lajide, 2000; Ogunleye, 2004;
Onolemhemhen, 2001; Ohazurike et al., 2003). In continuation of the research,
It has investigated the effect of palm oil for the control of S. zeamais
and C. maculatus in stored maize, C. cajan and V. unguiculata
MATERIALS AND METHODS
The insects (S. zeamais and C. maculatus) used for this analysis
were collected from infested stock of grains (Z. mays, C.
cajan and V. unguiculata) respectively stored at Federal College
of Agriculture Akure, Nigeria (28±3°C, 66% relative humidity).
Grains and Palm Oil Samples
Grains and oil samples were obtained from a local market in Akure, Nigeria.
The grains were picked to separate impurities from good ones. The palm oil was
filtered to remove impurities. The experiment was performed in the Chemistry
laboratory of Federal College of Agriculture, Akure, between May and October
2006. Twenty-five grams of the grain samples were placed into 45 plastic containers.
Fifteen containers were assigned for each grain.
Each of the grains in the containers were treated with palm oil, mixed thoroughly
to ensure adequate contact and labeled thus: T0 = 0.0 mL (control)
T1 = 0.1 mL, T2 = 0.2 mL, T3 = 0.3 mL, T4
= 0.4 mL. Ten (5 males and 5 females) of the insect species were introduced
into each of the containers, covered with net and held tightly together with
rubber band. The control grains were devoid of palm oil. Treatments were in
fives and three replicates.
Experiment and Data Analyses
Analyses performed on the design were damage assessment, mortality (Udo
et al., 2004), grain viability and oviposition rate (Ohazurike et
al., 2003). Statistical analyses were performed with the use of an SPSS
for windows 10.0.
RESULTS AND DISCUSSION
The results of the effect of palm oil on the mortality of S. zeamais
and C. maculatus on Z. mays and C. cajan and V. unguiculata,
respectively are depicted in Table 1-3.
Only the control experiment had no mortality, instead the insects increased
in number during the storage period. The reason for this is obvious-oviposition
of the insects. From observation, the treatments of 0.1 and 0.2 mL caused the
death of the insects, but that of 0.3 and 0.4 mL were the most potent inducing
100% mortality between 12 and 24 h. There was significant difference between
the mortality of pests by low quantities (0.1 and 0.2 mL) and that of relative
high quantities (0.3 and 0.4 mL).
|| Effect of palm oil on mortality of S. zeamais at different
time intervals (Zea mays)
|SEM: Standard error of mean
|| Effect of palm oil on mortality of C. maculatus at
different time intervals (Vigna unguiculata)
|| Effect of palm oil on mortality of C. maculatus at
various time interval (Cajanus cajan)
|| No. of exit holes (seed damaged) caused by storage pests
on the stored seeds after 6 mouth of treatment
Present results were in agreement with the observations made by Ohazurike
et al. (2003) and Udo et al. (2004) that employed the use of extracts
of J. curcas and candlewood respectively for the control and S. zeamais
and C. maculatus. Mixing of palm oil with Z. mays, C. cajan and
V. unguiculata, probably resulted in thin smooth oil coating on the treated
grains, which limited contact between the grains and the weevils. Death of the
weevils may have resulted from the interference with normal respiratory mechanizing
and starvation. There were no signs of oviposition of the storage pests in all
the different concentration levels during observation for 30 days, but there
were in the control experiments this was in agreement with results of Onolemhemhen
(2001). Reason for this could be attributed to the fact that all the insects
died after 48 h of treatment and so oviposition was not possible. Onolemhemhen
(2001) also reported that plant oil do not cause mortality of grain weevils,
but also impair oviposition and progeny emergence.
The control had the highest number of holes manifesting on the grains (Table 4). The reason was due to the existence of the pests which fed on them. Other results showed that the higher the quantity of palm oil, the lower the number of exit holes. There were limited contacts between the insects and the grains.
Control sample did not show any sign of viability since the embryo used for germination was destroyed by the pests, but there were signs of germination in the test experiments (Fig. 1). This observation showed that the use of palm oil in the storage of Z. mays, C. cajan and V. unguiculata against S. zeamais and C. maculatus does not affect the viability of seeds. The indication is that the oil can be used to preserve and protect grains in storage. This will ensure that undamaged and viable seeds are available for human and animal consumption, planting and distribution at periods of peak demand (Onolemhemhen, 2001).
|| Percentage viability of the stored grains
From the results obtained in this study, it could be concluded that the use of palm oil in pest management is safe to the environment, grain and animal, but not to pests. It is therefore necessary to make use of it as an alternative to chemical method of preservation of grains.