Background and Objective: Activities at mining soil induced extreme complication for environmental, physics, chemical and biology. Arbuscular Mycorrhizal Fungi (AMF) is mutualism symbiosis between fungi and roots of spermatophyta plants. The aim of this study was to assess indigenous AMF in mining contaminated sites Bukit Ngalau PT Semen Padang. The AMF Indigenous were assessed by direct field sampling and trap culture technique. Materials and Methods: The symbiosis may affect several other factors that influence the plants ecology, such as water availability, access to other nutrients, grazing resistance and tolerance to soil pathogens and pollutants. Results: Identification of indigenous AMF is a necessary step in a phytoremedial strategy in rehabilitation, especially in post mining soil. Conclusion: The AMF identified fell within Glomus, Gigaspora and Acaulospora taxa. Glomus was the dominant taxa with the highest spore abundance (38/50 g soil) compared to Gigaspora and Acaulospora.
PDF Abstract XML References Citation
How to cite this article
Mining is one of the flagship sectors plays an important role in Indonesian economy1. One of the biggest mining activity in west Sumatra is PT Semen Padang which is located in the Indarung Village, Lubuk Kilangan district, Padang. The mining activity negatively impacts the environment. Mining can lead to the destruction of habitats in surrounding areas such as loss of vegetation and destruction of soil horizons2. The negative impacts can be prevented and reduced by conducting a rehabilitation, ensuring the area mined is returned close to its original state. Mine rehabilitation have become as an important part of the sustainable development in many countries, especially through revegetation. However, the initiatives undertaken on lands that have been damaged (critical land) often encounter problems. This is because some plant species is difficult to adjust due to drastic changes in habitat. Environmental management is needed to overcome this conditions and minimizing the negative impacts of mining on the environmental also preserving eco diversity3. Thus required the use of technology to support the revegetation process as with the application of Arbuscular Mycorrhizal Fungi (AMF).
Arbuscular Mycorrhizal Fungi (AMF) as soil micro-organisms plays an important role in the ecosystem, including marginal land. The AMF provide the plant with water, soil mineral nutrients (mainly phosphorus and nitrogen) and pathogen protection. In exchange, photosynthetic compounds are transferred to the fungus4. Mycorrhizal an association between certain fungi with plant roots have an important role in increasing resistance to nutrient deficiency, droughtiness, extreme pH, heavy metals and able to improve the structure and biological properties of the soil2. The AMF help plants become established in harsh/degraded ecosystems, such as desert areas and mine spoils5.
The AMF can be found almost in most soils and generally do not have a specific host plants. The level and composition of the population is very diverse and influenced by the characteristics of the plant and environmental factors such as temperature, soil pH, soil moisture, phosphorus and nitrogen as well as the concentrations of heavy metals. Thus, each ecosystem is likely to contain the AMF of the same type or it could be different. In revegetation process, indigenous AMF is considered better than using AMF exogenous6. The AMF indigenous is a type that is found in association with plant roots naturally without human intervention in the process of initial infection between mycorrhizae with host plants. The AMF indigenous has a high potential to form extensive infection because they are more adaptive and have a higher tolerance to environmental with extreme conditions7. Identification of indigenous AMF is a necessary step in a phytoremedial strategy in rehabilitation, especially in post-mining soil. This study aims to assess of indigenous AMF in mining contaminated sites Bukit Ngalau, PT Semen Padang.
MATERIALS AND METHODS
Time and study site: The study was conducted from May-July 2018. Soil samples were collected from mining contaminated sites Bukit Ngalau, PT Semen Padang, Indarung, Lubuk Kilangan district, Padang, West Sumatera, Indonesia (Fig. 1).
Isolation of arbuscular mycorrhizal fungi (AMF): Isolation of indigenous AMF was carried out by taking a sample of the soil in the field according to the methods of the ICRAF8. The AMF isolate taken from roots of vegetation area with deep of soil about 5-20 cm (rhizosphere area). On each post sample will taken ±500 g of soil (composite). Soil samples were taken put in plastic and labeled in accordance with the type of plant as well as the date of sampling soil (Fig. 2).
Propagation of arbuscular mycorrhizal fungi (AMF)/ trapping: The AMF trapping was done using the "Open pot culture method" according to Brundrett et al.9. Cultures are grown in a greenhouse at least 1 months. Trapping is necessary step to obtain many healthy spores of colonizing fungi for identification and as inoculum to establish monospecific cultures.
Extraction and identification of arbuscular mycorrhizal fungi (AMF): Extraction and identification of spores from soil samples was carried out by referring to the pour and wet filter method10 with centrifugation modifications9. This pouring and filtering technique procedure, first is mixing the soil sample as much as 50 g with 500 mL of water, then stirring until the granules of the soil are destroyed. Then filtered in a set of filters with a size of 500, 125 and 63 μm sequentially from large to small size of filters set. The top filter is sprayed with water to make it easier for the spores to escape. Then the top filter was removed and a number of residual soil left on the 125 and 63 μm sieve was transferred to the centrifuge tube and centrifugation technique was carried out. Filter results in centrifugal tubes plus 60% glucose using pipettes. The centrifuge tube is closed and centrifuged at 3000 rpm for 5 min.
|Fig. 1:||Study sites Bukit Ngalau PT Semen Padang, West Sumatera, Indonesian|
|(Source : www.google.com/maps)|
|Fig. 2:||Isolation of AMF with plot 20×20 m2 at Bukit Ngalau, PT semen Padang, West Sumatera, Indonesia|
Then the supernatant solution was poured into 45 μm filter paper or Petri dish and observed under a binocular microscope for spore density (abundance) calculation. Observation of the characterization of FMA type is based on morphological characteristics of spores. The FMA was identified to the genus level with reference to morphological descriptions of AMF genus.
In this study found 3 genus of the AMF in the rhizosphere of 14 plants species at Bukit Ngalau, PT Semen Padang (Table 1) consists of Glomus, Gigaspora and Acaulospora.
|Table 1:||Genus of AMF were found associate with vegetation in mining contaminated sites Bukit Ngalau, PT Semen Padang|
|Fig. 3:||Genus of spores found associated with plants rhizosphere in Bukit Ngalau PT Semen Padang|
These types of AMF are associated with different types of plants. The greatest dominant genus found was from Glomus taxa which is associate with 8 species of vegetation with different habits and has the highest abundance of spore 38/50 g of soil. The Glomus population was found as the highest compare to other mycorrhizal genus Acaulospora and Gigaspora. On the other hand, Acaulospora and Gigaspora genus only found associate with 3 types of vegetation and the lowest value for spores abundance was from Acaulospora (9/50 g of soil) (Fig. 3).
This study found the AMF Indigenous from post mining land Bukit Ngalau that can be beneficial for rehabilitation of post mining land especially through revegetation. The result showed that Glomus was the dominant taxa with the highest spore abundance and form the association with most of plants at study site. Glomus is considered as a generalist species at contaminated areas. Glomus is also adapted to the stress caused by different concentrations of heavy metals11. Saidi et al.12 mentioned that some of Glomus species produces higher glomalin than the other species. Glomalin serve to protect the hyphae of drought and destruction attack by microbes. According to Husin et al.13, Glomus spores are also found dominant in various rhizosphere in the critical land of Sumatra.
All 14 plants found at Bukit Ngalau, PT Semen Padang showed a strong symbiotic relationship between plants species and AMF but significant differences were observed in the different of host plant species and the spore density varied among plant species. There be selectivity between host plants and AM fungi. The number of AM fungi detected was influenced by host plants under certain conditions14. Holste et al.15 also mentioned that the diversity and density of AMF spores would determine by the type of plants on the ground. Both of mycorrhiza and plant diversity can stimulate or retard each other14. The exudates that was produced by the roots of the host plant also can affect the germination of spores and the growth of hyphae. The root exudates produce a signal that can be responded by the AMF, if there is a match then the spores will germinate and grow to form hyphae16-18.
The highest abundance of Glomus spore may also have been influenced by trapping process. Trap cultures is an important factor influencing mycorrhizal development, especially spore formation19. Glomaceae isolates generally are the fastest genus to colonize plant roots. Meanwhile, Acaulosporaceae isolates generally colonized roots more slowly, at a rate more similar to spore-regenerating Gigasporaceae. Glomaceae isolates showed more extensive colonization than others isolate20. The extensive colonization from Glomus may be one of the reason why Glomus has higher abundance of spore compared to Acaulospora and Gigaspora. On trap culture, there are also some species present in the field soil that were lost after trap culture and the frequency and relative abundance of some species were quite different before and after trap culture19. This may due to differences in the ability of AMF to sporulate. According to Wang and Jiang21, Glomus are thought to require less time to produce spores than others species. Glomus spores germitate faster where it only take 4-6 days of germination9,22. The AMF indigenous found in this study is beneficial for the revegetation on post-mining soil. AMF indigenous can guarantee and help plants become established in harsh ecosystems such as post mining soil. In Indonesia, the government recommend to use native plants in land rehabilitation, this study will help the success of using native plants as rehabilitation strategy that associated with AMF.
There is three genera of AMF were found in the rhizosphere of vegetation at Bukit Ngalau, PT Semen Padang. The AMF identified fell within Glomus, Gigaspora and Acaulospora. Glomus was the dominant genus with the highest spore abundance (38/50 g soil) compared to both of Gigaspora and Acaulospora.
This study was supported by Andalas University who has given financial support for this study with the grant number 4917.01 $and PT Semen Padang for facility and access to study sites.
- Sheoran, V., A.S. Sheoran and P. Poonia, 2010. Soil reclamation of abandoned mine land by revegetation: A review. Int. J. Soil Sedim. Water, Vol. 3, No. 2.
- Bonfante, P. and A. Genre, 2010. Mechanisms underlying beneficial plant-fungus interactions in mycorrhizal symbiosis. Nature Commun., Vol. 1.
- Requena, N., E. Perez-Solis, C. Azcon-Aguilar, P. Jeffries and J.M. Barea, 2001. Management of indigenous plant-microbe symbioses aids restoration of desertified ecosystems. Applied Environ. Microbiol., 67: 495-498.
- Kartika, E., Lizawati and Hamzah, 2012. [Isolation, identification and Purification of Arbuscular Mycorrhiza Fungi (AMF) from coal post mining soil]. Bioplantae, Vol. 1.
- Ervayenri, Y. Setiadi, N. Sukarno and C. Kusmana, 1999. Arbuscular Mycorrhizal Fungi (AMF) diversity in peat soil influenced by land vegetation types. Proceedings of the International Conference on Mycorrhizas in Sustainable Tropical Agriculture and Forest Ecosystems, October 27-30, 1997, Bogor, pp: 85-92.
- Brundrett, M., N. Bougher, B. Dell, T. Grove and N. Malajczuk, 1996. Working with Mycorrhizas in Forestry and Agriculture. Australian Centre for International Agricultural Research, Canberra, Australia, ISBN: 1862301815, Pages: 374.
- Pacioni, G., 1992. Wet-Sieving and Decanting Techniques for the Extraction of Spores of Vesicular-Arbuscular Fungi. In: Methods in Microbiology, Volume 24: Techniques for the Study of Mycorrhiza, Norris, J.R., D.J. Read and A.K. Varma (Eds.). Chapter 16, Academic Press, San Diego, CA., USA., ISBN: 978-0-12-521524-4, pp: 317-322.
- Leal, P.L., M. Varon-Lopez, I.G. de Oliveira Prado, J.V. dos Santos, C.R.F.S. Soares, J.O. Siqueira and F.M. de Souza Moreira, 2016. Enrichment of arbuscular mycorrhizal fungi in a contaminated soil after rehabilitation. Braz. J. Microbiol., 47: 853-862.
- Saidi, A., E.F. Husin, A. Rasyidin and E. Eddiwal, 2015. Effect of Arbuscular Mycorrhiza Fungi (AMF) and the organic material to the glomalin production and the soil physical properties of ultisols. Int. J. Adv. Sci. Eng. Infor. Technol., 5: 361-366.
- Husin, E.F., R. Marlis, Trimitri, Auzan, Burhanuddin and Z. Zelfi, 2007. Observasi dan identifikasi spora Cendawan Mikoriza Arbuskula (CMA) pada berbagai rhizosfir di lahan kritis Sumatera. Proceedings of the Percepatan Sosialisasi Teknologi Mikoriza untuk Mendukung Revitalisasi Kehutanan, Pertanian dan Perkebunan, Mycorrhiza National, July 19-20, 2007, Bogor.
- Liu, R. and F. Wang, 2003. Selection of appropriate host plants used in trap culture of arbuscular mycorrhizal fungi. Mycorrhiza, 13: 123-127.
- Holste, E.K., K.D. Holl, R.A. Zahawi and R.K. Kobe, 2016. Reduced aboveground tree growth associated with higher arbuscular mycorrhizal fungal diversity in tropical forest restoration. Ecol. Evol., 6: 7253-7262.
- Giovannetti, M., L. Ayio, C. Sbrana and A.S. Citernesi, 1993. Factors affecting appressorium development in the vesicular–arbuscular mycorrhizal fungus Glomus mosseae (Nicol. and Gerd.) gerd. and trappe. New Phytol., 123: 115-122.
- Pinior, A., U. Wyss, Y. Piche and H. Vierheilig, 1999. Plants colonized by AM fungi regulate further root colonization by AM fungi through altered root exudation. Can. J. Bot., 77: 891-897.
- Armansyah, A. Anwar, A. Syarif, Yusniwati and R. Febriamansyah, 2018. Exploration and identification of the indigenous Arbuscular Mycorrhizae Fungi (AMF) in the rhizosphere of citronella (Andropogon nardus L.) in the dry land regions in West Sumatra province, Indonesia. Int. J. Adv. Sci. Eng. Inform. Technol., 8: 85-92.
- Tian, H., J.P. Gai, J.L. Zhang, P. Christie and X.L. Li, 2009. Arbuscular mycorrhizal fungi associated with wild forage plants in typical steppe of Eastern Inner Mongolia. Eur. J. Soil Biol., 45: 321-327.
- Hart, M.M. and R.J. Reader, 2002. Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytol., 153: 335-344.
- Wang, M. and P. Jiang, 2015. Colonization and diversity of AM fungi by morphological analysis on medicinal plants in Southeast China. Scient. World J., Vol. 2015.
- Delvian, D., 2010. Presence of arbuscular mycorrhizal fungi in coastal forest based on the salinity gradients. J. Ilmu Dasar, 11: 133-142.