MATERIALS AND METHODS

For data collection, independent random samples had been drawn from urban localities of the Punjab among adolescents aged from 10-18 years. Subjects were selected from government and private schools, in order to recruit them from different socioeconomic strata. A total of 1225 adolescents aged 10-18 years consisted 634 boys and 591 girls were enrolled from 10 schools of urban areas of Punjab, to identify accurate and significant anthropometric predictors for cardiovascular diseases. Data were collected from eight districts (Amritsar, Faridkot, Moga, Muktsar, Gurdaspur, Fatehgarh Sahib, Bathinda and Ferozpur) in the state of Punjab. Written informed consent was obtained from all the parents of subjects prior to their participation. Study was carried out through subsequent visit in the school of randomly selected subjects. The study was approved by Guru Nanak Dev University appropriate research ethics committee in the year 2010. The recruitment of subjects was done on school to school basis. Data collection and personal interviews were also done with each subject. General information about name, sex, date of birth, religion, caste, address and information related to life style habits like smoking, alcohol intake and food habits were recorded on the pre-designed Performa. All measurement and interviews have been taken by single investigator with same instrument, therefore, intra and inter observer variability for taking measurements were very negligible.

Anthropometric measurement: The anthropometric measurements like height (cm), weight (kg), waist circumference (cm) and hip circumference (cm) were taken on each individual using standard anthropometric techniques (Singh and Bhasin, 1968; Weiner and Lourie, 1981). The age of the adolescents was determined directly from their reported date of birth in the school records. The height was measured using anthropometric rod with the standing erect position with the head in ear-eye plane. The reading was then, recorded to the nearest 0.1 cm. The weight of the subject was measured in kilograms by making them stand on a weighing machine with minimal clothing. Weight was recorded with an allowance deducted for clothing to the nearest 0.5 kg. Waist circumference was measured using a steel tape. The measurement was taken mid-way between the inferior margin of the last rib and crest of the ilium in a horizontal plane with relaxed abdomen. The tape was fitted snuggly without compressing the soft tissue. Hip circumference of the subject was taken with steel tape fitted around the pelvis at the point of maximal protrusion of buttocks while the subject was standing with his/her feet close to each other. The readings were recorded for waist and hip circumferences to the nearest 0.1 cm. The values for BMI expressed as the ratio of body weight divided by body height squared (kg m–²), Waist to Hip Ratio (WHR) defined as waist circumference (cm) divided by hip circumference (cm), similarly waist to height ratio (WHtR) defined as waist circumference (cm) divided by height (cm) and the conicity index (C index) was determined by measuring weight, height and waist circumference using the following mathematical equation:

Physiometric measurement: The blood pressure was measured with standardized mercury sphygmomanometer and a stethoscope by following the recommendations of American Heart Association (Kumar et al., 2011). Blood pressure was measured in sitting posture with the hands resting on examining table with the cubital fossa supported at the level of the heart. The stethoscope was placed over the brachial artery pulse, proximal and medial to the cubital fossa and below the bottom edge of the cuff (i.e., about 2 cm above the cubital fossa). On the basis of circumference of the participant’s arm, a regular adult or small or medium cuff was chosen. The systolic blood pressure is defined as the appearance of the first sound (Korotkoff phase 1) and diastolic blood pressure is defined as the disappearance of sound (Korotkoff phase 5). The average of the two blood pressure measurements was used as the estimate of Systolic Blood Pressure (SBP) and Diastolic Blood Pressure (DBP) in the present analysis. However, the variation of two readings was negligible. Mean arterial blood pressure (MBP) was simply estimated as DBP+(SBP-DBP)/3. To calculate the pulse rate, the radial artery at the wrist was used to feel the pulse. It was count over one minute.

Statistical analysis: Data were analyzed using SPSS software 17.0 version. Base line characteristics were summarized with mean and standard deviations. The comparisons of baseline characteristics were compared with t-statistics. The sensitivity, specificity, likelihood ratio and odds ratio were calculated for every anthropometric indicator. Likelihood ratio is defined as sensitivity/(1-specificity). The Confidence Intervals (CI) were also calculated to determine the lower and upper limit of predictive ability of these anthropometric indicators. The p<0.05 level was selected as the criterion of statistical significance.

RESULTS

Of the total 1225 adolescents aged 10-18 years investigated, 51.76% were boys (634) and 48.24% were girls (591). The descriptive data of both boys and girls are shown in Table 1. The mean age of boys and girls are 13.59±2.34 and 13.90±2.45 years, respectively.

Table 1:	Baseline characteristics of the Punjabi adolescent aged 10-18 years

Table 2:	Prevalence of hypertension by sex in Punjabi adolescents aged 10-18 years through obesity parameters
Values in parenthesis indicate percentage

The mean values of height, weight, waist circumference and conicity index are significantly (p<0.001) higher in boys as compared to girls. However, only hip circumference was significantly (p<0.001) higher in girls. The mean values of other variables such as BMI, WHR, SBP, DBP and pulse rate are consistently higher in boys as compared to girls but the differences are not statistically significant.

The mean prevalence of arterial hypertension for total subjects with normal BMI, waist circumference and WHtR were 36.73% (37.23% in boys and 36.21% in girls), 38.37% (39.75% in boys and 36.89% in girls) and 37.63% (37.85% in boys and 37.39% in girls), respectively. Similarly, the mean prevalence of arterial hypertension for total overweight subjects in both sexes with respect to BMI, waist circumference and WHtR were 3.35% (4.10% in boys and 2.55% in girls), 2.05% (1.89% in boys and 2.20% in girls) and 2.78% (3.31% in boys and 2.20% in girls), respectively. The mean prevalence of arterial blood pressure for total obese subjects with respect to BMI, waist circumference and WHtR were 2.94% (4.10% in boys and 1.69% in girls), 2.61% (3.79% in boys and 1.35% in girls) and 2.61% (4.26% in boys and 0.85% in girls), respectively (Table 2).

Comparisons were made between normotensive and hypertensive with respect to anthropometric and physiometric characteristics (Table 3). The mean differences of BMI, waist circumference, SBP, DBP, MBP and WHtR were statistically significant between normotensive and hypertensive boys, girls and total subjects. However, the differences of means of other variables such as age and height in boys; weight, hip circumference and pulse rate in girls and total subjects have found significant (p<0.001) between normotensive and hypertensive. The mean values of hypertension related risk factors such as BMI (18.16 vs. 16.95 for boys and 18.28 vs. 16.92 for girls), WC (64.46 vs. 62.83 for boys and 63.77 vs. 60.59 for girls), pulse rate (77.45 vs. 76.51 for boys and 77.74 vs. 75.85 for girls), WHtR (0.42 vs. 0.40 for both boys and girls) among hypertensive groups observed significantly (p<0.001) higher as compared to normotensive groups.

The prevalence of obesity with respect to the anthropometric indicators such as BMI, waist circumference and waist/height ratio for boys and girls are given in Table 4. The percentage prevalence of overweight has been observed in boys 5.36, 3.00, 6.31 and in girls 4.23, 3.55, 3.55 using BMI, waist circumference and waist/height ratio as the indicators, respectively.

Table 3:	Comparison of baseline characteristics of hypertensive and normotensive punjabi adolescent aged 10-18 years
BMI: Body mass index, Waist circum.: Waist circumference, Hip circum.: Hip circumference, WHR: Waist hip ratio, SBP: Systolic blood pressure, DBP: Diastolic blood pressure, MBP: Mean arterial blood pressure and WHtR: Waist to height ratio

Table 4:	Prevalence of Obesity by sex in Punjabi adolescents aged 10-18 years through obesity parameters
Values in parenthesis indicate percentage

Table 5:	Sensitivity, specificity and likelihood ratio with confidence interval of anthropometric indicators associated with hypertension
BMI: Body mass index, WC: Waist circumference, WHtR: Waist to height ratio

Whereas, the percentage prevalence of obese has been identified as in boys 5.51, 4.42, 5.52 and in girls 2.28, 1.86, 1.35 using BMI, waist circumference and waist/height ratio as the indicators, respectively. However, as a whole the highest percentage of overweight (4.98%) and obese (4.33%) have been observed using waist/height ratio and BMI as the indicators, respectively.

Sensitivity and specificity for predicting hypertension by different anthropometric indicators are summarized in Table 5. Sensitivity for predicting hypertension by different anthropometric indicators such as BMI, waist circumference and WHtR cutoff points varied in total subjects 63.5-72.2%; in boys 64.0-76.6%; in girls 58.1-65.6% and specificity varied in total subjects 58.9-59.6%; in boys 57.1-58.2%; in girls 60.7-61.0%. The positive likelihood ratio varied in total subjects 1.54-1.759; in boys 1.49-1.80; in girls 1.48-1.68. The values of specificities are almost equal for all anthropometric indicators.

Table 6:	Odds ratio of hypertension in obese punjabi adolescents aged 10-18 years determined by the appropriable cutoff points of anthropometric indicators

However, sensitivity is higher for waist circumference (72.2%) for total subjects, in boys (76.6%) and in girls (65.6%). The likelihood ratio has also maximum for waist circumference in total subjects (1.759) and in girls (1.683). Therefore, waist circumference would be considered better predictor of hypertension in adolescents.

Logistic regression was performed to see the different anthropometric indicators such as BMI, waist circumference and WHtR independently increased the risk of having hypertension among boys, girls and total subjects (Table 6). The odds ratios for BMI, waist circumference and WHtR were significant (p<0.001) in boys, girls and total subjects. The odds ratios of waist circumference in boys (4.35), girls (2.98) and total subjects (3.73) were higher as compared to BMI and WHtR. Therefore, prevalence of hypertension was significantly higher in those adolescents who had higher waist circumference.

DISCUSSION

Hypertension, overweight and obesity are the major health problems among present day Punjabi adolescents population. The major objective of present study is to compare three anthropometric indicators such as BMI, waist circumference and WHtR and to identify which anthropometric indicator is better discriminator for obesity and cardiovascular risk among adolescents. However, BMI was traditionally been used to measure obesity and hypertension in different epidemiological studies, whereas, other indicators such as waist circumference and waist to height ratio have also been popularly used by different investigators (Afridi et al., 2003; Sidhu et al., 2004; Ashwell and Hsieh, 2005; Bigaard et al., 2005; Feldstein et al., 2005; Welborn and Dhaliwal, 2007; Alhamdan, 2008; Sung et al., 2008; Badaruddoza and Kumar, 2009; Beck et al., 2011). According to BMI cut-off points, 37.23% boys and 36.21% girls and 36.73% of total subjects with normal BMI have been identified with hypertension, respectively. Also according to waist circumference cut-off points, 39.75, 36.89 and 38.37% of boys, girls and total subjects with normal waist circumference have been identified with hypertension. The similar results according to waist to height ratio cut-off points, 37.85, 37.39 and 37.63% of boys, girls and total subjects have been identified with hypertension with normal WHtR. Therefore, waist circumference was more important to identify high risk cardiovascular disease among adolescent subjects. Similar to present results many investigators used waist circumference as the important criteria to identify the high risk subjects for obesity and hypertension (Yalcin et al., 2005; Badaruddoza and Sawhney, 2009; Badaruddoza and Kaur, 2012; Huxley et al., 2010). Furthermore, genetic, environments and dietary intake have also contributed a significant proportion of anthropometric and physiometric variation (Tassaduqe et al., 2004; Mahajan et al., 2009; Afoakwah and Owusu, 2011; Badaruddoza and Patharia, 2012). However, all these anthropometric indicators (BMI, waist circumference and WHtR) were good predictors but population and ethnic specific differences have also been found. Although, by comparing the sensitivity, specificity and likelihood ratios of these three indicators, not so much differences between BMI and waist circumference have been found in both sexes and combined subjects. Therefore, these two anthropometric indicators (BMI and waist circumference) can be used to predict the elevated blood pressure in adolescents. Many other studies in different population have also suggested that BMI and waist circumference would be good predictor for hypertension in adolescents (Katzmarzyk et al., 2004; Sung et al., 2006; Beck et al., 2011; Gupta and Kapoor, 2012). For an odds ratio analysis from the present study it has also been observed that all three anthropometric indicators (BMI, waist circumference and WHtR) have significant association with hypertensive obese Punjabi adolescents in both and combined sexes. However, increasing risk of hypertension in obese adolescents was found to be associated with increasing waist circumference in both and combined sexes, although, adolescent boys are more prone to develop hypertension with increasing waist circumference as compared to girls. The odds ratio of boys was much higher (4.35) as compared to girls (2.95).

CONCLUSION

In conclusion, waist circumference is more important and better anthropometric indicator for detecting cardiovascular risk factor among adolescent boys as compared to adolescent girls.

ACKNOWLEDGMENT

The financial assistance to SK Brar, Maulana Azad National Fellowship from UGC, New Delhi is gratefully acknowledged. Conflict of Interest: No conflict of interest was declared.