Metals Concentrations in Eggs of Domestic Avian and Estimation of Health Risk from Eggs Consumption
Salwa A. Abduljaleel
M. Shuhaimi- Othman
This study is focused on evaluating the trace metals levels in eggs of four species of domestic birds that collected from commercial farm located in Kajang city, Malaysia. The concentrations of Al, Cr, Ni, Zn, As, Cd and Pb were measured using inductively coupled plasma mass spectrometry (ICP-MS). Additionally, this paper carries out probabilistic risk analysis methods to quantify As, Zn, Cd and Pb bioaccumulation in egg content to assess the range of exposures for the people who consume the contaminated eggs. The models applied include a probabilistic bioaccumulation model to account for this metals accumulation in egg and a human health exposure and risk model that accounts for hazard quotient and lifetime risk for humans consuming contaminated eggs. Generally, result showed that eggs in four species accumulated relatively dissimilar levels of metals. Zn is found in high levels while, Cd burden occurred in less levels in all birds eggs. Quail eggs were gathered elevated levels of Zn, Al and Pb. However, As exist in high concentration in chicken eggs. The associated risk was assessed using Hazard Quotient (HQ). Values of hazard quotient were ranged 0.5-0.03, 0.09-0.03, 0.1-0.06 and 0.2-0.03 for As, Zn, Cd and Pb, respectively. The risk quotient revealed that the intake of the heavy metals by eating eggs does not pose any apparent threat to the local people as none of the HQ of the heavy metals exceeds the limit of 1.
August 03, 2011; Accepted: October 19, 2011;
Published: December 10, 2011
Global environmental pollution through heavy metal lead to an increased interest
in metals contamination of food-stuffs and amongst them eggs which symbolize
an important part of human's diet especially children due the avian eggs is
an important source of nutrients, containing all of the proteins, lipids, vitamins
and minerals (Sparks, 2006). Food safety is a major
public concern worldwide in recent decades due the growing demand for food safety
has stimulated research regarding the risks associated with consumption of foods
contaminated with pesticides, heavy metals or toxins (DMello,
2003). Additionally, Eggs used as evidence for environmental pollution since
they can accumulate the heavy metals from diet and surrounding environment (Burger
et al., 2009). Besides, avian eggs have been shown to be vulnerable
to external application of toxicants and represent local exposure of the adults
have laid them (Burger, 1993). Some of the heavy metals
that are known as potentially toxic include aluminum, arsenic, cadmium and lead
and other essential metals such as, zinc, nickel and chromium those play a definitive
role in the intrinsic mechanisms regulating vital biological processes, toxic
elements can be harmful even at low concentrations when ingested over a long
period of time (Nolan, 1983; Young,
2005). Therefore, exposure to metals through consumption eggs may pose health
risk especially for high eggs consumption populations. According to USEPA
(1997) high levels of inorganic arsenic in food or water can be fatal. A
high level is 60 parts of arsenic per million parts of food or water (60 ppm).
Arsenic damages many tissues including nerves (peripheral polyneuropathy, axonal
degeneration), stomach, intestines and skin. As well as, long term exposure
to lower levels of cadmium in air, food, or water leads to a buildup of cadmium
in the kidneys. Further, cadmium is cancer-causing and potentially mutation-causing,
with severe sub lethal and lethal effects at low environmental concentrations
(Eisler, 1985). Mills (1983)
claimed that excess zinc can cause some feeding disorders and diseases and impairs
the immune system. Several researchers, including Al-Saleh
(1994) and Fraser et al. (2006) have reported
that lead exposure causes neurotoxicity, which is characterized by histological,
ultrastructural and neurochemical changes in the central nervous system, as
well as behavioral shortfall. However, In Malaysia urban residents consume chicken
and eggs more frequently than their rural counterparts, more men than women
consumed chicken and eggs more frequently (Norimah et
al., 2008). There is a lack of studies regarding metal levels in eggs
of domestic birds in this area of Malaysia and whether these levels represent
a human health risk.
Consequently, the objectives of this study were to determine concentration and distribution of Aluminum (Al), Chromium (Cr), Nickel (Ni), Zinc (Zn), Arsenic (As), Cadmium (Cd) and lead (Pb) in egg content of chicken, quail, guinea fowl and pigeon to assess differences among metals content for domestic avian species and to estimate the health risk to local consummators from ingestion of metal contaminated eggs.
MATERIALS AND METHODS
Reagents: The reagents with super quality, analytical grade Nitric acid (70%) and hydrogen peroxide (30%) were acquired from Merck (Darmstadt, Germany) along with the stock standard solutions of Al, Cr, Ni, Zn, As, Cd and Pb in concentrations of 1,000 mg L-1. All the plastic and glassware were cleaned by soaking in dilute HNO3 (10%) and were rinsed with de-ionized water prior to use.
Apparatus: A Perkin Elmer model Elan 9000 inductively coupled plasma-mass spectrometry (ICP-MS, USA) was used in current study. After calibrating the apparatus with standard solutions derived from commercial materials, it was optimized according to the manufacturing standards the cones and tubes were carefully cleaned to get rid of any possible residues.
Sample collection and preparation: Eggs sample from chicken (Gallus
gallus), quail (Coturnix coturnix japonica), guinea fowl (Numida
meleagris) and pigeon (Columba livia) were collected from commercial
farm located in a rural area in Kajang city(latitude 2°59N, longitude
101°47E), Selangor, Malaysia. About 36 eggs (for each species) were
chosen randomly during the period (from May to October 2010), all eggs were
weighed, chicken egg (40.79±4.01 g), guinea fowl egg (38.55±1.75
g), quail egg (11.38±0.87 g) and pigeon egg (12.16±1.35 g). To
analyze the metals in egg content according to method previously described by
Burger and Eichhorst (2005) with slight modification,
the eggs were washed vigorously with de ionized water, then open carefully the
eggshell separated from egg content, the egg content were homogenizes and put
it in Petri dishes the samples were drying at 70°C for 24 h the egg content
grain to powder by mortar, 0.5 g of the sample soaked in nitric acid 70% and
hydrogen peroxide 30% overnight in room temperature, the digested completed
in bloke thermostat (150°C) for 4 h until solution were clear.
||Analytical results for the Certified Reference Materials (CRM)
and its certified values for each metal (μg g-1 dry weight)
|The CRM for Al is not available
After cooling the solution was diluted to 50 mL with de ionized water, then
were filtrated throw 0.45 μm acid resistant filter paper. The solutions
were stored at 4°C for later metal analysis. Concentration of Aluminum (Al),
Chromium (Cr), Nickel (Ni), Zinc (Zn) Arsenic(As), Cadmium (Cd) and lead(Pb)
Were determined by inductively couple plasma-mass spectrometry (ICP-MS, model
Perkin-Elmer Elan 9000 A). Each analysis was carried in duplicate, standard
and blank samples were analyzed every 20 sample. All concentration were expressed
in μg g-1 on dry weight basis. The accuracy of the applied analytical
method was validated by accurate analysis of Certified Reference Materials (CRM)
for lobster hepatopancreas (TORT-2, National Research Council Canada). All the
runs were carried out in triplicate. The results obtained on the SRMs are showed
in Table 1. The recoveries of all the metals were satisfactory.
Health risk estimation: To estimate the human health risk from consuming
metal contaminated eggs, the methodology for estimation of target hazard offers
an indication of the risk level due to pollutant exposure, this method was available
in US EPA Region 111 Risk based concentration table (USEPA,
2000). It is described by following equation:
where, EF is exposure frequency (from 365 days/year for people who eat egg
7 times a week to 52 days/year for people who eat egg one time a week. ED is
the exposure duration (70 years), equivalent to the average lifetime; FIR is
the food ingestion rate (egg: 37.2 g/person/day). C is the metal concentration
in egg content (μg g-1), RFD is the oral reference dose (USEPA,
1997, USEPA, 2000); WAB is the average body weight
(64 kg, the reference weights for the age categories were derived from several
local studies in Malaysia (Lim et al., 2000)
and TA is the averaging exposure time for non carcinogens(365 day/yearxED).
If HQ>1.00, indicating that there is a potential risk associated with that
metal (Khan et al., 2008). The lifetime Cancer
Risk (CR) for arsenic was obtained by using the chemical Slope Factor (SF) (USEPA,
1997) and the estimated metal exposure dose mg/kg/day (EED) in the following
Statistical analysis: All calculations were performed using SPSS for Windows (vers. 18.0, SPSS Ltd., Woking, Surrey, UK). The descriptive statistics (mean values, standard deviation) for values of egg content were analyzed by one-way analysis of variance followed by the Tukey honestly significant difference test. Differences were considered significant at the p<0.05 level.
Metal analysis in eggs: Result demonstrated that disparity in metals concentrations in eggs among four birds species, among all the heavy metals, Zn concentration was maximum and Cd was minimum in four species eggs, the highest concentration of Zn were occurred in quail eggs, followed by chicken and guinea fowl however, their level does not significantly differ among species. Al concentration was significantly (p<0.05) highest in quail eggs followed by those of chicken and pigeon. Mean while, Cr was occurred in analogous values in chicken and guinea fowl eggs (3.2 and 3.6 μg g-1), respectively followed by those of guinea fowl and pigeon. The same situation was observed in nickel concentration (Table 2). As and Cd levels in eggs of birds were found in less values compared to those of other metals. For As chicken showed the highest value. While for Cd concentration, quail presented the highest once. As far as, the highest values for Pb were obtained in Quail eggs while the lowest value found in guinea fowl eggs.
Health risk estimation: According to Norimah et
al. (2008) that daily consumption of chicken egg for one person in Malaysia
about one egg (medium) daily, which equivalent (without eggshell) average (37.2
g/person/ day), we suggest that daily consumption of other birds eggs was in
the same rate. Metals (As, Cd and Pb) were choose to estimate the THQ due these
metals may inadvertently enter the food chain and pose health risk to human
(Zhuang et al., 2009) and Zn because it has high
concentration among diagnosed metals in current study. Target hazard quotient
and cancer risk of As were presented in Table 3.
||Mean concentration of heavy metals (μg g-1
dry weight±SD) in egg content of domestic avian species
|Values with different letters within arrow are significantly
|| Health risk estimate for As ingestion from domestic birds
|| Health risk estimate for Zn ingestion from domestic birds
In order to estimate the human health risk from consuming metal-contaminated
egg, we used the reference dose of metals RFD and estimated metal exposure dose
EED (mg/kg/day). The THQ values of As in egg of domestic bird were ranged from
0.5 to 0.03 for people who eat eggs 7 times a week, from 0.3 to 0.02 for people
who eat eggs 4 times a week and from 0.2 to 0.005 for people who consume eggs
one time a week (Table 3). For Zn, TQH values ranged from
0.07 to 0.05 for people who eat eggs 7 times a week, from 0.06-to 0.03 for people
who consume eggs 4 times a week and from 0.03 to 0.007 when human eat eggs one
time a week (Table 4). For Cd the values of THQ reached from
0.12 to 0.063 for eggs consumed in 7 times a week, from 0.07 to 0.04 in 4 time
a week and 0.025-0.008 in one time a week of egg consumption (Table
5). While THQ values of Pb have been ranged from 0.2 to 0.04, 0.1-0.02 and
0.03-0.004 for people who eat egg 7, 4 and 1 time a week respectively (Table
|| Health risk estimate for Cd ingestion from domestic birds
|| Health risk estimate for Pb ingestion from domestic birds
||HQ (hazard quotient) values when the consumption of eggs more
than one eggs/person/day
Risk to human health from the intake of metal contaminated eggs was characterized
using hazard quotient HQ is a ratio of determined dose of a pollutant to the
dose level (a reference dose or RfD). If the ratio is less than 1 like in Zn,
Cd and Pb, there will not be any noticeable risk (Table 7).
Conversely, an exposed population of concern will experience health risks if
the dose is equal to or greater than the RfD (Khan et
Metals concentrations in avian eggs: Demonstration of trace elements
in eggs may be an important instrument for nutritionists and environmentalists.
Thus, current study provide a base information of the composition of domestic
avian eggs in trace elements, such information was rare before in Malaysia.
Present study confirmed that a large variation in metals concentrations among
domestic avian eggs, this differences may be resulted from feeding behavior
and different ability for birds to ingest soil and grass and the metabolic activities
among different bird species (Nisianakis et al.,
2009). In this study, diet, geographical location, or environmental pollution
could not have contributed to such differences due all birds were fed a common
diet and kept at the same range area free from any local pollution. Concentration
of Zn and Ni in chicken egg content in present study reached 34.22 and 1.11
μg g-1, respectively were higher than values previously recorded
by Van Overmeire et al. (2006), Meanwhile, Pb
and Cd concentration in current study in chicken eggs content get to (0.42,
0.05 μg g-1, respectively) were less than values reported by
Fakayode and Olu-Owolabi (2003). Arsenic burden was
found in high concentration in chicken egg content than pigeon egg content,
similar observation was also reported by Nisianakis et
al. (2009). According to Malaysian Food Act (1983)
concentration of Cd, Pb and Zn were lower than the recommended legal limit (1,
2 and 100 μg g-1, respectively). As concentration in chicken
egg was 0.3 μg g-1 and these results are obviously lower than
the permitted limits 2.0 ppm (ANZFA, 2001).
Health risk estimation: Some of the heavy metals such as Zn, Cr, Ni
and Cu act as micro-nutrients at lower concentrations, they become toxic at
higher concentrations. Small amounts of these elements are common in our environment
and diet and are actually necessary for good health but large amounts of any
of them may cause acute or chronic toxicity (poisoning). Heavy metal toxicity
can result in damaged or reduced mental and central nervous function, lower
energy levels and damage to blood composition, lungs, kidneys, liver and other
vital organs. Long-term exposure may result in slowly progressing physical,
muscular and neurological degenerative processes that mimic Alzheimer's disease,
Parkinson's disease, muscular dystrophy and multiple sclerosis. Repeated long-term
contact with some metals or their compounds may even cause cancer (IOSHIC,
The estimated THQ values in present study seem to indicate that consumption
of these egg does not implicate an appreciable human health risk for the consumer
due THQ values of (As, Zn, Cd and Pb) were less than 1, cancer risk SF for arsenic
in this study ranged 10-4-0-6 (Table 3)
the US EPA considers cancer risk that are below about 1 chance in 1,000,000
(1x10-6) to be so small as to be negligible and risks above 1x10-4
to be sufficiently large that some sort of remediation is desirable. Excess
cancer risks that range between 1x10-6 and 1x10-4 are generally considered to
be acceptable (USEPA, 1991). If we suggest that consumption
of chicken eggs will be increase from 1 egg to 2 or 3 eggs/day/person. Then
values of THQ of Zn, Cd and Pb were still in permission level except for Arsenic
value which it value exceed to 1.2 and 1.7 respectively (Table
6) this indicates that human health risk might be of concern in case of
increased eggs consumption.
We conclude that the concentration of Al, Cr, Ni, Zn, As, Cd and Pb were varied among the egg of domestic bird. The eggs of quail were accumulated high levels of Al, Zn and Pb compared to those in other birds eggs, meanwhile, Cd was occur in less concentration in the egg of chicken, guinea fowl and pigeon . From the standpoint of human health As, Zn, Cd and Pb. THQ values (<1) show a situation without risk to the consumer in the area of study. Further ecotoxicological studies are needed on the eggs of species in different area of Malaysia.
We take this opportunity to thank the Department of School of Environmental Science and Natural Resources, UKM, Malaysia for kindly providing all of the samples collected. The author wish to acknowledge the financial support provided by the Iraqi Ministry of Higher Education, Iraq.
ANZFA, 2001. Australia New Zealand food authority. Wellington, New Zeland 6036.
Al-Saleh, I.A.S., 1994. The biochemical and clinical consequences of lead poisoning Med. Res. Rev., 14: 415-486.
CrossRef | Direct Link |
Burger, J., M. Gochfeld, C. Jeitner, S. Burke and C.D. Volz et al., 2009. Mercury and other metals in eggs and feathers of glaucous-winged gulls (Larus glaucescens) in the Aleutians. Environ. Monit. Assess., 152: 179-194.
CrossRef | Direct Link |
Burger, J. and B. Eichhorst, 2005. Heavy metals and selenium in grebe egg from Agssiz national wild life refuge in northern Minnesota. Environ. Monit. Assess., 107: 285-295.
PubMed | Direct Link |
Burger, J., 1993. Metals in avian feathers bioindicators of environmental pollution. Rev. Environ. Contam. Toxicol., 55: 203-311.
D'Mello, J.P.F., 2003. Food Safety Contaminants and Toxins. CABI Publishing, Wallingford, Oxon, UK., ISBN-13: 9780851996073, Pages: 452.
Eisler, R., 1985. Cadmium hazards to fish, wildlife and invertebrates: A synoptic review. Biological report 85(1.2). US Fish and Wildlife Service, Laurel, MD., USA.
Fakayode, S.O. and I.B. Olu-Owolabi, 2003. Trace metal content and estimated daily human intake from chicken eggs in ibadan, Nigeria. Arch. Environ. Health: Int. J., 58: 245-251.
CrossRef | Direct Link |
Fraser, S., G. Muckle and C. Despres, 2006. The relationship between lead exposure, motor function and behaviour in Inuit preschool children. Neurotoxi. Terato., 28: 18-27.
PubMed | Direct Link |
IOSHIC, 1999. Metals: In Basics of Chemical Safety. Chapter 7, International Labour Organization, Geneva.
Khan, S., Q. Cao, Y.M. Zheng, Y.Z. Huang and Y.G. Zhu, 2008. Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China. Environ. Pollut., 152: 686-692.
CrossRef | PubMed | Direct Link |
Lim, T.O., L.M. Ding, M. Zaki, A.B. Suleiman and S. Fatimah et al., 2000. Distribution of body weight, height and body mass index in a national sample of Malaysian adults. Med. J. Mal., 55: 108-128.
PubMed | Direct Link |
Malaysian Food Act, 1983. Malaysian Food and Drug. MDC Publishers Printer Sdn. Bhd., Kuala Lumpur.
Mills, C.F., 1983. The physiological basis of trace element deficiency disease. Trace Elements Anim. Product. Vet. Pract., 7: 1-9.
Nisianakis, P., I. Giannenas, A. Gavriil, G. Kontopidis and I. Kyriazakis, 2009. Variation in trace element contents among chicken, turkey, duck, goose and pigeon eggs analyzedby inductively coupled plasma mass spectrometry (ICP-MS). Bio. Trace Elem. Res., 128: 62-71.
PubMed | Direct Link |
Nolan, K.R., 1983. Copper toxicity syndrome. J. Orthomol. Psychiatry, 12: 270-282.
Direct Link |
Norimah, Jr. A.K., M. Safiah, K. Jamal, S. Haslinda and H. Zuhaida et al., 2008. Food consumption patterns: Findings from the Malaysian Adult Nutrition Survey (MANS). Malays. J. Nutr., 14: 25-39.
PubMed | Direct Link |
Sparks, N.H.C., 2006. The hen's egg-is its role in human nutrition changing? World's Poult. Sci. J., 62: 308-315.
Direct Link |
USEPA, 1991. Role of the Baseline Risk Assessment in Superfund Remedy Selection Decisions. US Environmental Protection Agency, Washington DC., USA.
USEPA, 1997. Exposure Factors Handbook. Office of Research and Development, National Center for Environmental Assessment, Cincinnati, Ohio.
USEPA, 2000. Guidance for Assessing Chemical Contaminant Data for use in Fish Advisories: EPA 823-B-00-007. 3rd Edn., Vol. 1, Office of Science and Technology and Office of Water, USEPA, Washington, DC., USA.
Van Overmeire, I., L. Pussemier, V. Hanot, L. DeTemmerman, M. Hoenig and L. Goeyens, 2006. Chemical contamination of free-range eggs from Belgium. Food Add. Contamn., 23: 1109-1122.
PubMed | Direct Link |
Young, R.A., 2005. Toxicity profiles: Toxicity summary for cadmium. Risk Assessment Information System. University of Tennessee.
Zhuang, P., M.B. McBride, H. Xia, N. Li and Z. Li, 2009. Health risk from heavy metals via consumption of food crops in the vicinity of dabaoshan mine, South China. Sci. Total Environ., 407: 1551-1561.
CrossRef | PubMed | Direct Link |