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Research Article
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Effects of Brewery, Textile and Paint Effluent on Seed Germination of Leafy Vegetables-Amaranthus hybridus and Celosia argentea (Amaranthaceae)
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K.O. Ogunwenmo,
O.A. Oyelana,
O. Ibidunmoye,
G. Anyaso
and
A.A. Ogunnowo
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ABSTRACT
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Effluent-laden water bodies have increasingly been used as sources of irrigation for arable crops to ensuring year round food production and sufficiency. Toxicity of living tissues may result from substances accumulated in the growth medium through the food chain. In order to assess the suitability or otherwise of some industrial wastewater for irrigation purposes, germination experiment was performed on seeds of Amananthus hybridus and Celosia argentea presoaked in 50 and 100% concentration of brewery, textile and paint effluent for 30 min to 3 h. Longer duration of seeds in presoaked medium (3 h) increased germination rate (0.92) and percentage (95%) of A. hybridus significantly (p<0.05) to optimum level in 50% diluted brewery effluent. Though, the effluent generated gradual increase in germination of C. argentea with increasing presoaking period, the maximum germination (25 and 35%) was below the control untreated seeds (45%). Fifty percent textile effluent favoured germination in A. hybridus up to the control level (70%) at 2 h with higher rate (0.63). Germination decreased (45%) significantly (p<0.05) beyond 2 h. One hundred percent and 50% textile effluent significantly decreased (p<0.05) germination in A. hybridus (5-20%) and C. argentea (5-10%), respectively and totally toxic to C. argentea at 100%. The rate and percentage seed germination of A. hybridus and C. argentea decreased significantly (p<0.05) as the presoaking period increased in paint effluent becoming toxic beyond 1 and 1½ h, respectively. Industrial effluent may be environmentally harmful if not properly treated or diluted.
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How
to cite this article:
K.O. Ogunwenmo, O.A. Oyelana, O. Ibidunmoye, G. Anyaso and A.A. Ogunnowo, 2010. Effects of Brewery, Textile and Paint Effluent on Seed Germination of Leafy Vegetables-Amaranthus hybridus and Celosia argentea (Amaranthaceae). Journal of Biological Sciences, 10: 151-156. DOI: 10.3923/jbs.2010.151.156 URL: https://scialert.net/abstract/?doi=jbs.2010.151.156
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INTRODUCTION
Environmental pollution constitutes a great health hazard to human, animals
and plants with local, regional and global implications (Irshad
et al., 1997). Pollution has adverse effects on land, water or air
and its biotic and abiotic components. Water pollution may result from municipal,
agricultural or industrial wastes containing organic and inorganic chemical
substances, dissolved or suspended solids (Terry, 1996;
Nebel and Wright, 1998; Moeller, 2004).
Industrial waste effluents are discharged into water bodies with multiple effects
and changes to water physicochemical parameters (pH, temperature, Biological
Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Dissolved Oxygen (DO), Total
Suspended Solids (TSS), heavy metals, turbidity etc.) (Nebel
and Wright, 1998) supporting aquatic life and organisms that depend on them.
Organic wastes contain heavy metals, ammonia, salts and low molecular weight
organic acids which may be toxic to plants (Fuentes
et al., 2004).
Increasing population and consequent increase in food demand had necessitated
intensive year round food production. Hence, irrigation of arable land and crops
from water bodies is indispensable to ensuring food sufficiency. Increasingly,
effluent-laden water bodies have become sources for crop irrigation systems
sometimes preferred for added nutrients from municipal wastes, distillery or
agro-based industries (Veer and Lata, 1987; Pathak
et al., 1999; Subramani et al., 1999;
Ramana et al., 2002a; Shrestha
and Niroula, 2003). Nevertheless, pollutants may also change the properties
and composition of soil and micro-flora (Kisku et al.,
2000; Shrestha and Niroula, 2003). More so, plants
respond to pollution stress and metabolize pollutants differently (Zhu,
2001; Ramana et al., 2002b) by varying mechanisms
of uptake, translocation and accumulation (Salt et al.,
1998; Rehman et al., 2008).
Phytotoxicity results from intoxication of living tissues by substances accumulated
from the growth medium (Chang et al., 1992).
The toxic substance may be further bio-accumulated and magnified in the food
chain with dire consequences for human. Therefore, it is imperative to evaluate
the toxic effects of wastewater and their suitability for irrigation of crop
plants. The transition between dormancy and germination represents a critical
stage in the life cycle of crop plants which controls population dynamics and
productivity (Radosevich et al., 1997; Keller
and Kollmann, 1999) constituting an important ecological and commercial
trait (Holdsworth et al., 2008).
Thus, seed germination bioassay of two leafy vegetables (Amaranthus hybridus
L. and Celosia argentea L. (Amaranthaceae) was performed in three
industrial wastewater (brewery, textile and paint). These plants are popular
vegetables widely cultivated in domestic gardens and farms both in rural and
urban areas of Nigeria (Maynard, 1983). Amaranthus
hybridus is a robust annual herb (Olorode, 1984; Epenhuijsen,
1974) popularly cultivated throughout the world (Rehm
and Gustav, 1991; Lehman, 1989) for its nutritional
value (Oguntona, 1998; Ifon and Bassir,
1979). Celosia argentea is a short lived annual herb, slow growing
and more drought resistant than A. hybridus. It is mostly cultivated
in the South-Western part of Nigeria (Oguntona, 1998;
Rehm and Gustav, 1991; Epenhuijsen,
1974).
This study was conducted to assess the effects and toxicity levels of brewery, textile and paint effluent on seed germination of A. hybridus and C. argentea with a view to determining their suitability for crop irrigation. MATERIALS AND METHODS Seeds of A. hybridus and C. argentea were obtained from the Institute of Agricultural Research and Training (IAR and T) Ibadan, Oyo State, Nigeria in March 2006. Untreated effluents (100%) were collected from Brewery, Textile and Paint Industries at Ikeja and Ogba, Lagos in March 2006 and analyzed for physicochemical parameters at the Analytical Research Laboratory, Babcock University. They were diluted with water to make 20, 50 and 80%. Germination studies: Four hundred viable seeds of each species were randomly selected from the stock. Preliminary tests were performed with 20, 50, 80 and 100% effluent concentration before 50 and 100% were selected for the bioassay. A set of 20 seeds of A. hybridus and C. argentea was presoaked in 50 and 100% brewery, textile and paint effluent concentrations for 30 min, 1, 1½, 2 and 3 h. Each was carried out in duplicates. At the end of each time-treatment, the seeds were placed between folds of moistened filter paper in glass Petri dish at room temperature, 28±2°C in the Biology Research Laboratory, Babcock University. The preparation was moistened with effluent every 12 h and observed for radicle emergence every 24 h as indicative of germination. Control: A set of 20 untreated, intact seeds of A. hybridus and C. argentea was soaked in tap water for 30 min, 1, 1½, 2 and 3 h, respectively. At the end of each soaking period, the seeds were sown in regularly moistened filter paper in Petri dish at room temperature, 28±2°C. The preparations were watered every 12 h and the emergence of radicle was observed every 24 h as indicative of germination. Computation and statistical analysis: The rate and percentage of seed germination were calculated for each species from the formula: Rate (coefficient velocity of germination), r = x1-x0/nxt1+x2-x1/n x t2 + x3 x2/n x t3
.. xn- xy/nxtn where, x is the number of seeds germinated per total number of seeds, n, emerged on a particular number of day t1 and xn - xy is the difference between present and last germination (not necessarily previous, where, x2 = 0, r = x3 - x1/n x t3) at tn; r ranges from 0-1. Percentage seed germination (%) = x/nx100 The values were subjected to one-way Analysis of Variance (ANOVA) to determine statistical significance. Statistical analysis were performed using SPSS for Windows, version 14.0. (SPSS Inc. Chicago, IL. USA). RESULTS
Germination increased significantly (p<0.05) above the control level (70%)
with increase in presoaking period of seeds of A. hybridus in 50 and
100% brewery effluent concentration. The highest percentage (95%) and rate (0.92)
of germination was obtained at 3 h with 50% diluted effluent. There was significant
decrease (p<0.05) in the percentage and rate of germination of C. argentea
in 50 and 100% brewery effluent. The highest percentage (35%) of germination
occurred at 3 h in 100% effluent below the control level (45%) at 2 h (Figs.
1a-c and 2a-c).
Germination increased in A. hybridus with increasing presoaking period up to control levels (70%) at 2 h in 50% textile effluent with a considerable higher rate (0.63).
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Fig. 1: |
Percentage
germination of Amaranthus hybridus and Celosia argentea treated
with brewery, textile and paint effluent (a) brewery, (b) textile and
(c) paint |
| Fig. 2: | Rate
of germination of Amaranthus hybridus and Celosia argentea
treated with brewery, textile and paint effluent (a) brewery, (b) textile
and (c) paint |
Germination decreased (45%) significantly (p<0.05) beyond 2 h. One hundred percent and 50% effluent treatments significantly decreased germination (p<0.05) in A. hybridus (5-20%) and C. argentea (5-10%), respectively. One hundred percent textile effluent was completely toxic to C. argentea (Fig. 1, 2). Paint effluent inhibited seed germination with increasing presoaking period. It was completely toxic at 100% effluent concentration in A. hybridus and beyond 1 h in 50% effluent. Germination was very low in C. argentea (5-15%) and toxicity resulted beyond 1½ and 2 h seed treatment in 100 and 50% paint effluent, respectively (Fig. 1, 2). The analysis of physicochemical parameters of the effluents is shown in Table 1. DISCUSSION Transcriptional and post-transcriptional processes take place in dry seeds following desiccation. After-ripening processes and post-after-ripening hormone-signaling as well as hormone-independent environmental signals (light, temperature) determine germination potentials (Holdsworth et al., 2008).
Germination marks the resumption of metabolic activities following imbibition,
cell division and enlargement. Such activities include hydration of sub-cellular
organelles, enzyme, protein and RNA syntheses, hormone (abscisic acid) and enzyme
(endo-B-mannanases) controlled degradation of embryo surrounding tissues and
the endosperm, mobilization of reserves from cotyledons and/or endosperm, protein
kinases activated signal cascades, activation or redistribution of metabolites
within the embryonic axis and hormone (gibberellins) dependent embryo axial
elongation (Black et al., 2000; Noggle,
2002). These activities involve increased water and oxygen uptake. Hence,
the growth medium containing water and dissolved chemical substances impacts
significantly on the success of seed germination. More so, plants absorb, transport
and accumulate these chemical substances differently. Similarly, duration of
seed presoaking period in medium influence germination rates positively or negatively
and requires effective monitoring (Ugborogho and Ogunwenmo,
1999).
Whereas the seed coat of A. hybridus permits increased germination with
increasing presoaking period up to 2 h under natural conditions, C. argentea
affords only marginal increase. Consequently, diluted brewery wastewater enhanced
seed germination in A. hybridus maximally between 30 min and 3 h presoaking.
Generally, distillery effluent had been found to enhance seed germination and
/ or yield in groundnut (Ramana et al., 2002a),
different vegetables (Ramana et al., 2002b),
wheat, rice, pea and lady finger (Pathak et al.,
1999; Pandey et al., 2007), due to presence
of essential nutrients (copper, sulphates and nitrates) and absence of toxic
elements. Conversely, brewery wastewater retarded germination below control
in C. argentea. Impregnable seed coat seemed to prevent nutrient enrichment
of germination from brewery wastewater in C. argentea. Naturally, C.
argentea was generally more resistant to drought than A. hybridus.
Two hour presoaking period in diluted textile mill was critical for effective
seed germination in A. hybridus while higher concentration up to 100%
in textile effluent inhibited it. Whereas A. hybridus selectively permitted
seed germination in textile mill wastewater, C. argentea was inhibited
by diluted textile wastewater becoming toxic at higher concentration. The mechanism
of resistance to toxic chemicals is relatively unclear but may be due to the
ability of different plants to detoxify toxic substances, particularly heavy
metals or exclude them from the roots. Textile mill wastewater enhanced germination
with increasing concentration in Cicer arientum while at the same time,
it decreased root, shoot and seedling lengths, fresh and dry weights and to
some extent dry matter accumulation (Nawaz et al.,
2006). Untreated textile wastewater decreased the germination of Turnip
and Brassica with increasing concentration while it had no adverse effects on
Radish. Similarly, the fresh and dry weights of the three vegetables decreased
significantly though Brassica weighs more than the control in almost all concentrations
except 100% (Rehman et al., 2008). Heavy metals
(e.g. Pb2+, Cd2+, Cr2+, Ni2+) even
at low concentrations may result in phytoxicity by impairing a range of cellular
activities and reducing the uptake of other essential nutrients (Palacios
et al., 1998; Kadar and Kastori, 2003). Oil
and grease may act jointly with other salts and organic compounds to increase
the osmotic potential thus, reducing the amount of water and oxygen available
in the medium for maximum imbibition critical to onset of germination.
Paint effluent generally inhibited germination in both species. These may be
due to presence of some salts (-Cl-1), oil and grease and toxic heavy
metals (Pb, Cd, Cr and Ni) which retard germination and growth. Unlike textile
mill, C. argentea slightly permitted germination in 2 and 1½ h
presoaking period in 50 and 100% paint effluent. On the other hand, A. hybridus
could only afford germination up to 1 h in 50% paint effluent while 100% was
completely toxic. Plants response to effluent stress and their uptake, translocation
and accumulation of the metabolites vary (Salt et al.,
1998; Ramana et al., 2002b) depending on
their genetic make-up and eco-physiology. Even closely related cultivars (P-91
and P-2000) of the same species (C. arientum) responded differently to
the same effluent (Nawaz et al., 2006).
Overall, brewery effluent improved seed germination in A. hybridus while textile mill was only effective at lower concentrations up to 50% at 2 h presoaking period. Paint would require clean-up, maximum dilution and short presoaking period below 30 min to yield any appreciable result in A. hybridus. None of the effluents was suitable for C. argentea but may be slightly tolerant to paint diluted effluent where water is scarce. Generally, paint and textile wastewater inhibited seed germination and became toxic at higher concentrations.
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