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Morphological studies in Lycopersicon esculentum Mill. lines in Southwestern Nigeria



O.J. Lawal, A.E. Ayodele and K.S. Chukwuka
 
ABSTRACT

Morphological studies of thirty lines of Lycopersicon esculentum Mill were carried out in order to find out characters, which may be used for the delimitation of the cultivars in Nigeria. The quantitative and qualitative characters studied include leaf type, leaf length, leaf shape, leaf breadth, petiole length, number of leaves per plant, number of leaflets per leaf, number of serration per leaflet days taken for germination, days taken for first picking, stem diameter, fruit diameter, fruit shape, fruit colour, stem type, stem colour, stem texture, leaf arrangement and growth habit. Morphological characters that indicate close affinity amongst the cultivars include leaf type, leaf base, leaf shape, leaf apex, leaf margin, leaf arrangement stem colour, fruit colour, number of calyx and corolla. However, the features that are taxonomically relevant in the delimitation of lines of L. esculentum are fruiting habit, fruit shape and presence or absence of ridges on the fruits. The three cultivars recognized are Ibadan local, hybrid and Roma VF. Numerical analysis of the qualitative and quantitative characters data using cluster analysis-average linkage, single linkage, complete linkage and centroid method delimit all the thirty lines into three major groups which conform to the results of the morphological analysis.

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  How to cite this article:

O.J. Lawal, A.E. Ayodele and K.S. Chukwuka , 2007. Morphological studies in Lycopersicon esculentum Mill. lines in Southwestern Nigeria. Journal of Biological Sciences, 7: 737-744.

DOI: 10.3923/jbs.2007.737.744

URL: https://scialert.net/abstract/?doi=jbs.2007.737.744

INTRODUCTION

Lycopersicon esculentum Mill. (Solanaceae) is an important vegetable crop grown mainly for its edible fruits and sometimes leaves. As a popular vegetable crop, it ranks second in importance to the potatoes in many countries (Heywood, 1982). Tomato was probably introduced into Nigeria through early trade mission of the Portuguese to Africa and freed slaves from West Indies, but over the years, it has assumed a prominent position in the farming systems and diets of Nigerians (Denton and Swarup, 1983). Lycopersicum is a relatively small genus consisting of a little over ten species including the cultivated tomato (L. esculentum) and other closely related wild species (Rick, 1979; Phillips and Rix, 1993). These species are native to South America but widely cultivated and naturalized in warm temperate and tropical regions of the world (Phillips and Rix, 1993; Wagner et al., 1999). Apart from L. esculentum, the other species common to the West African region is L. cerasiforme Duval (Hutchinson and Dalziel, 1963).

Tomatoes are good sources of vitamins A and C and can help alleviate deficiencies of these vitamins in many developing countries (Villareal, 1971). Furthermore, the leaves are boiled and applied as poultices to relieve pain (Dalziel, 1948) while the leaves are pulped for embrocating areas of local pain (Burkill, 2000). The infusion of the leaves is also used in the treatment of gonorrhoea. In Nigeria, the leaves are considered antibiotic and fungicidal on sores (Burkill, 2000).

There are many cultivars of L. esculentum, some introduce and some local (Quinn and Crowther, 1976). These are generally classified according to their growing habit, fruit shape, cultivation method (outdoor or greenhouse production) and whether the fruit is a fresh market or for processing. There are however local cultivars which evolved from varieties introduced by the Portuguese. The introduced cultivars are often more susceptible to disease while the local cultivars are more tolerant to several diseases (Norman, 1992). As with some other vegetable crops in cultivation, for example, Solanum Linn. species, extensive crosses have been done on L. esculentum all over the world resulting in several varieties, cultivars and complexes. In Nigeria, while different cultivars have been recommended for specific ecological zones of the country (Adelana, 1976; Adelana and Oyedokun, 1979) the cultivars in Western Nigeria are known to be different especially in fruit shape, from those grown in Southern Nigeria as a result of geographical or ecological isolation (Norman, 1992).

The objective of this study is to examine the available lines of L. esculentum in the southwestern part of Nigeria and attempt to use the morphological characters so obtained to delimit the lines.

MATERIALS AND METHODS

Collection of materials: Seeds of twenty-five lines of L. esculentum were collected from the Genetic Resources and Biotechnology unit of National Horticultural Research Institute (NIHORT) Idi-Isin, Ibadan, Nigeria. In addition, five ripe tomato fruits of different shapes were bought at Bodija market, Ibadan, Oyo State, Nigeria. A list of the thirty lines is presented in Table 1. The seeds were removed, washed and spread on sheets of Newspaper under the shade to dry for one week.

All the thirty lines of tomato seeds were sown in planting pots filled with top soil of 10 kg each. Watering was done regularly. After four weeks of sowing, seedlings were transplanted to the field. The seedlings were planted at a spacing of 60 x30 cm on one meter wide beds as recommended by Adelana (1976). The planting was carried out in the Botanical Nursery of the Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria.

Data collection: After six weeks of planting, quantitative morphological characters which include plant height, leaf length, leaf breadth, petiole length, stem diameter were measured using standardized metre rule and Venier Caliper. The average of ten measurements taken for each character was recorded.


Table 1:

List of lines of L. esculentum studied

The average number of leaves per plant, number of serration per leaflet, primary branches per plant, days taken for germination, days taken for flowering, days taken for first picking, number of calyx and corolla were also recorded. At maturity (at about twelve weeks), qualitative morphological characters which include leaf apex, leaf base, leaf shape, leaf margin, leaf texture, leaf arrangement, leaf orientation, fruiting habit, stem type, stem colour, fruit shape, fruit size, fruit colour (both matured and immature fruits), fruit lobes, fruit pedicel area and fruit pistil scar were assessed.

Numerical taxonomy
Selection of Operational Taxonomic Units (OTUS):
Altogether, forty characters were used for the analyses. These included both qualitative and quantitative morphological characters. Data matrix comprising of forty characters by thirty OTUS was used.

Data analysis: The data matrix was analysed using hierarchical cluster analysis. The agglomeration schedule employed were the average linkage between groups, complete linkage, single linkage and the centroid method Euclidean distance was used to measure the distance between OTUS

In this statistics Xij is the character state value for character I in j and Xik is that for line K. The programmes were run on a portable personal computer using the statistical package for social sciences (SPSS) for windows.

RESULTS

Table 2-7 and Fig. 1-4 give the summaries of the results of this study. The average plant height recorded was 99.6 cm (Table 2). The fruiting habit of the plant was found to affect the weight. Determinate types (Hybrid and Roma VF) with erect stems are taller than the indeterminate type with prostrate stem. Leaves are longer, wider and broader in Ibadan local cultivar than in other cultivars recognised but Roma VF and the hybrid had higher number of leaves than Ibadan local cultivar (Table 2). Number of leaflets per leaf (7) is uniform for all the three cultivars (Table 1) except in some few lines with nine leaflets. Seed germination occurred five days after planting, Ibadan local cultivar germinated first and hence produced flowers and fruits earlier than the other two cultivars. The average days taken for germination is 6.5 (Table 2).


Table 2:

Quantitative morphological features of Lycopersicon esculentum


Table 3:

Morphological characters of each of the three cultivars recognised


Table 4:

Diagnostic features of each of the three cultivars recognised

The petiole length ranges between 0.60-2.3 cm. Fruits of Ibadan local cultivar are the largest among the three cultivars, the fruits diameter ranges between 3 and 10 cm. There was an overlap in the number of fruits per bunch among the cultivars. Some lines had between 3 and 5 fruits while others had between 5 and 8 fruits (Table 2).


Table 5:

Grouping of OTU ’S according to the method of analysis

A = Roma VF, B = Hybrid, C = Ibadan local, AV = Average linkage, CL = Complete linkage, CM = Centroid method, SL = Single linkage

The qualitative morphological studies showed a very close relationship between the thirty lines. All the lines had the same floral, leaf and stem morphology (Table 2). From the observations, the lines are thus grouped into three different cultivars; these are Ibadan local, hybrid and Roma VF. Morphological features used in the delimitation of cultivars are the presence or absence of ridge on the fruit, fruiting habit, leaf orientation and general fruit morphology (Table 3 and 4).

Morphological description of the cultivars recognised
Ibadan local:
This is an annual herb with indeterminate growth. It grows up to 1 m in height. The stem is much branched and prostrate, green with hairy surface. The leaves are compound, ovate, alternate, petiolate, up to 7.0 cm long and 4.0 cm wide, margin serrated, apex acute, base oblique. The petiole is up to 1.6 cm long.

Flowers are yellow, pedicel length 0.6 cm long, calyx green, four to five and gamosepalous. Corolla yellow, four to five and gamopetalous. The fruit is a berry, deep green when immature, red when mature, up to 12 cm in diameter, flattened to slightly flattened, multilobed and strongly depressed, pistil scar irregular and blossom end shape indented.

Hybrid: Growth is determinate. It grows up to 1.7 m in height. The stem is erect, green and glabrous to hairy. The leaves are compound with 7-9 leaflets, ovate, alternate, petiolate, up to 6.5 cm long and 4.0 cm wide, margin serrated, apex acute, base oblique. The petiole is up to 1.8 cm long. Calyx is green, corolla yellow, four to five and fused. The fruit is a berry, light green when immature, red when mature, up to 8.5 cm in diameter, heart shaped, bilobed, slightly depressed, pistil scar stellate and blossom end shape flat.

Roma VF: This is an annual herbaceous plant with determinate growth. The height is about 1.2 m. The stem is erect, green, glabrous and up to 8.0 cm in diameter. The leaves are compound with 7-9 leaflets, ovate, alternate,petiolate up to 6.8 cm long and 4.1 cm wide, margin serrated, apex acute, base oblique.


Table 6:

Qualitative morphological characters of lines of L. esculentum studied

The lines are as numbered in Table 1

Table 7:

Quantitative morphological characters of lines of L. esculentum studied


Fig. 1:

Dendrogram using average linkage (between groups). The lines are as numbered in Table 1


Fig. 2:

Dendrogram using average linkage. The lines are as numbered in Table 1

The petiole is up to 2.1 cm long. The calyx is green and corolla yellow. Fruit is a berry, green when immature, red when mature, up to 8.5 cm in diameter, high round, unlobed without ribbing, pistil scar dot-like and blossom end shape flat to pointed.


Fig. 3:

Dendrogram using complete linkage. The lines are as numbered in Table 1


Fig. 4:

Dendrogram using the centroid method. The lines are as numbered in Table 1

Numerical analysis: The results of hierarchical clustering procedures are presented in form of phenograms, which are diagrams of relationship. The abscissa shows the spacing out of the lines employed in the study while the ordinate on the other hand represents degree of similarity between and among the lines. The numbers represent the lines as in Table 1. Figure 1-4 are phenograms of 40 x30 data matrix obtained from Average Linkage Cluster Analysis (ALCA), Complete Linkage Cluster Analysis (CLCA), Single Linkage Cluster Analysis (SLCA) and Centroid Method (CM), respectively using qualitative morphological characters.

Three phenetic clusters are recognised from the four methods of analysis used (Fig. 1-4). These are groups A, B and C, which correspond to ROMA VF, Hybrid and Ibadan local, respectively (Table 5). Although branching occurred at low phenon levels which show broad similarities among the cultivars, there are some lines that stand as outliers and do not correspond to any group of the three cultivars recognised. Also some lines were found to overlap in all the three cultivars.

DISCUSSION

The results of the morphological studies showed a very close relationship among the cultivars. From the study, qualitative leaf, stem and floral morphological characters are similar in all lines while the cultivars exhibit variations in growth habit and fruit morphology (Table 3 and 4). All the cultivars have compound, ovate leaves with serrated margins, acute apex and oblique base with alternate arrangement (Table 3).

The floral morphology showed the presence of four to five, green, gamosepalous calyx and four to five, yellow, gamopetalous corolla. Stamens alternate with petals and there are two or more joined carpels in all the cultivars. There is a little variation in stem morphology among the three cultivars. Ibadan local has a deep green, hairy and prostrate stem; Roma VF has a light green, glabrous and erect stem while the Hybrid was found to be intermediate between the two cultivars. Roma VF with erect stem exhibited a determinate growth, Ibadan local has indeterminate growth. Fruit morphology is the major qualitative character used in the delimitation and identification of each cultivar. The major diagnostic feature is the fruit shape. Ibadan local is slightly flat to flat in shape with many lobes on the fruit surface. The Hybrid is heart shaped and it is bilobed while Roma VF is high round with smooth unlobed surface.

The shapes of the scar on the fruits also vary among the cultivars; it is irregular in Ibadan local, stellate in the hybrid and do-like in Roma VF. Ibadan local has indented blossom end shape, the hybrid has pointed end while Roma VF has a flat end shape. The average plant height recorded was 99.6 cm. The determinate cultivars with erect stems were found to be taller than the indeterminate cultivars with prostrate stems. Ibadan local germinated first and produced flowers and fruits earlier than the other cultivars. The average days taken for germination was 6.5 and this falls within the range of 6-7 days reported by Norman (1992). Number of leaflets per leaf was uniform for all the cultivars which were predominantly seven except in some lines with eleven leaflets. The number of serration per leaflets for all the cultivars was eleven.

The result of the clustering analysis showed that branching occurred at very low phenon line, which suggests a broad and over all similarities among the cultivars. This can be attributed to Hybridization and ability of tomato to self pollinate (Heywood, 1982). This analysis conforms to the delimitation of the thirty lines into three major groups using morphological characters but there are discrepancies in the sorting of the lines into the component groups. These discrepancies are not unconnected with the clustering methods used i.e., Average Linkage (AL) complete Linkage (CL), Single Linkage (SL) and Centroid Method (CM). In the average linkage, there is always an attempt to relate the new OTU to an average value of extant group rather than to the similarity or difference within it. The complete Linkage or Farthest Neighbour clustering on the other hand joins an OTU to a cluster based on the greatest similarity with the farthest OTU already within the same cluster (Lance and Williams, 1976b). The centroid method has a marked defect in that there is the tendency towards Chaining (Lance and Williams, 1976a) in which most of the OTUs join an initial cluster one at a time. This does not produce a convenient hierarchical classification of the OTUs.

Three groups of L. esculentum were recognised from this study. The delimitation of these groups was based entirely on the fruiting habit and fruit morphology i.e. fruit shape and presence or absence of ridges (lobes) on the fruits. Ibadan local, which is commonly cultivated in southern Nigeria, is an annual indeterminate plant with prostrate stem and flattened shaped fruits which many ridges. Roma VF popularly called Hausa tomato because of its prevalence in Northern Nigeria is an annual determinate plant with erect stem and high round to heart shaped fruits with no ridge (unlobed fruits). Because of its intermediate attributes between the two existing cultivars in Nigeria, the third group of the cultivars studied is recognized as a Hybrid in this study. The hybrid is a determinate plant with erect stem and pear shaped fruits with two to three lobes.

REFERENCES
Adelana, B.O. and O. Oyedokun, 1979. Variety and environment interactions in Western Nigeria tomatoes. Exp. Agric., 15: 285-287.

Adelana, B.O., 1976. Effects of plant density on tomato yields in Western Nigeria. Exp. Agric., 12: 43-47.

Burkill, H.M., 2000. The Useful Plants of West Tropical Africa. 2nd Edn., Royal Botanical Garden, Kew, PP: 293-294.

Dalziel, J.M., 1948. The Useful Plants of Tropical Africa. Crown Agents for the Colonies, London.

Denton, L. and V. Swarup, 1983. Tomato cultivation and its potential in Nigeria. Acta Hortic., 84: 257-271.

Heywood, V.H., 1982. Popular Encyclopedia of Pants. Cambridge University Press, Cambridge.

Hutchinson, J. and J.M. Dalziel, 1963. Flora of West Tropical Africa. 2nd Edn., Crown Agents for Overseas Government and Administration, London, ISBN: 0-85592-020-3, pp: 23-27.

Lance, G.N. and W.T. Williams, 1967. A general theory of classificatory sorting strategies. 1. Hierarchical systems. Hierarchical Syst. Compt. J., 9: 373-380.
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Norman, J.C., 1992. Tropical Vegetative Crops. Academic Press, London.

Phillips, R. and M. Rix, 1993. Vegetables. Pan Books, London.

Quinn, J.G. and P.C. Crowther, 1976. An evaluation of tomato cultivars suitable for paste production in Northern Nigeria. Trop. Sci., 18: 13-35.

Rick, C.M., 1979. Biosystematic Studies in L. esculentum and Closely Related Species of Solanum. Academic Press, London.

Villareal, R.L., 1971. In: Search of breakthroughs for vegetables production. Proc. Coll. Laguna Phillipines, pp: 42-59.

Wagner, W., L. Darral, R. Herbst and S.H. Sohmen, 1990. Manual of Flowering Plants of Hawaii. University of Hawaii Press, Honolulu, pp: 808.

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