Subscribe Now Subscribe Today
Research Article
 

Quinolone Resistant Staphylococcus aureus in Okigwe, Imo State Nigeria



O.C. Ugbogu, V.O. Nwaugo , A. Orji and N. Ihuoma
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
ABSTRACT

The antimicrobial spectrum of quinolones namely ceporex, ciprofloxacin, tarivid, peflacine and nalidixic acid on Staphylococcus aureus isolated from hospital patients in Okigwe was studied. Out of 79 samples examined Staphylococcus aureus was isolated from 50(71.42%). Out of 17 urethral swab isolates 15(88.27%), 10(58.8%), 12(70.5%), 8(47.05%) and 8(47.05%) were resistant to ceporex, ciprofloxacin, tarivid, peflacine and nalidixic acid, respectively. The percentage of the of the S. aureus isolates resistant to each quinolone was 62, 64, 74, 44 and 88% for ceporex, ciprofloxacin tarivid, peflacine and nalidixic acid, respectively. Isolates from urine, semen and vagina were less resistant to the quinolones.

Services
Related Articles in ASCI
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

O.C. Ugbogu, V.O. Nwaugo , A. Orji and N. Ihuoma , 2007. Quinolone Resistant Staphylococcus aureus in Okigwe, Imo State Nigeria. Journal of Biological Sciences, 7: 697-700.

DOI: 10.3923/jbs.2007.697.700

URL: https://scialert.net/abstract/?doi=jbs.2007.697.700

INTRODUCTION

Attempts to control diseases by chemotherapy through the use of antimicrobial agents particularly antibiotics have results in increased prevalence of resistance to these agents (Levy, 1998). Staphylococcus aureus is a Gram-positive, catalase positive, coagulase positive non motile coccus bacterium that causes a variety of human infections and is a major cause of surgical wound infections and epidermal skin diseases in newborn infants. They live as commensals in anterior naves of more half the population of humans (Doig, 1981) and the cocci spread from these sites into the environment by hands, handkerchief, clothing and dust. S. aureus is an opportunistic pathogen in the sense that it causes infection most commonly in tissues and sites with lowered host resistance (Burnett et al., 1990). Wound infections can be severe and the organism can invade the blood stream with consequent seedling of other sites such as heart valves, causing endocarditis (Prescott et al., 1996).

Various studies have been conducted to study the antimicrobial resistance pattern of S. aureus and it has been shown to be resistant to β-lactam antibiotics, aminoglycosides and macrolides (Atkinson and Lorian, 1984; Maple et al., 1989). S. aureus strains carry a wide variety of multidrug resistance genes on plasmids, which can be exchanged and spread among different species of Staphylococci (Neihart et al., 1988).

The quinolones are a group of synthetic antimicrobial agents that have gained wide usage (Boreherding et al., 1996). Shortly after the introduction of the fluoroquinolones into clinical practice, strains expressing resistance to these compounds were detected in particular among isolates of methicillin-resistant. S. aureus (MRSA), which frequently exhibit multiple additional resistance determinants to unrelated antimicrobial agents (Trucksis et al., 1991).

In developing countries such as Nigeria where drugs are readily available to consumers across the counter with or without prescription from medical practitioners, drug resistance is a serious problem. Misuse of antimicrobial drugs has been associated with high prevalence of drug resistance among the Staphylococci (Nnochiri, 1973; Paul et al., 1982). Researchers have reported that some hospital strains of S. aureus are resistance to all clinically useful antibiotics except vancomycin. Some workers have also reported the presence of vancomycin resistant S. aureus strains (Aury-Damon et al., 1998).

This study reports the resistance of Staphylococcus aureus hospital isolates in Okigwe to quinolones.

MATERIALS AND METHODS

Study area: This research was carried out in Okigwe Imo state, Nigeria between March and October 2006.

Antibiotics and media used: Antibiotic discs used and their concentrations were as follows: Tarivid (10 mg/disc), ceporex (30 mg/disc), peflacine (10 mg/disc), nalidixic acid (30 mg/disc) and ciprofloxacin (30 mg/disc). These discs were obtained commercially. Optun disc (Optun laboratories, Aba Nigeria) were used. The media used were Nutrient agar (Oxoid), Mannitol Salt agar (Difco), Bacto-peptone (Oxoid) and Mueller Hinton agar (Antec Diagnostics India) and were prepared according to manufacturers instructions.

Sample collection: Ear, vaginal, urethral, wound and carbuncle swabs were collected from patients attending General hospital Okigwe and Winners Hospital Okigwe using sterile swab sticks (Evepon). Urine, semen and stool samples were also collected from patients in the same hospitals using sterile specimen bottles. All specimens were transported to the Microbiology Laboratory of Abia State University and cultured within 3 h of collection. A total of 79 samples were isolated and characterized for presence of bacteria.

The specimens were inoculated on mannitol salt agar plates and Nutrient agar plates with sterilized wire loop to obtain discrete colonies. The plates were incubated at 37°C for 24 h under aerobic conditions. After 24 h of incubation, the culture plates were examined recording appearance, size, colour and morphology of colonies. Grams stain reaction, catalase test and coagulase tests were carried out on isolates. Isolates that were gram-positive cocci, catalase positive and coagulated human plasma were considered as Staphylococcus aureus (Chigbu and Ezeronye, 2003; Uaboi-Egbenni, 2003).

SUSCEPTIBILITY OF ISOLATES TO THE QUINOLONES

Antimicrobial sensitivity test was carried out on all isolates using the paper diffusion technique. A 0.2 mL of the 12 h peptone water culture of the test organism was used to inoculate on sterile Mueller Hinton agar plate. This was spread over the entire surface of the agar plate using a sterile glass spreader and allowed to dry for about 15 to 30 min. The antibiotic discs were then placed on the agar using sterile forceps. Each disc was placed far from each other so as to avoid their zones of inhibition from coalescing into the other. The set up was then incubated at 37°C for 24 h to observe the zones of growth inhibition. The Inhibition Zone Diameter (IZD) was measured in millimeters and interpreted by the standard of National Committee for Clinical Laboratory Standards (NCCLS) (Cheesbrough, 2002).

RESULTS

Out of 79 samples collected, Staphylococcus aureus was isolated from 50 representing 71.42%. Thirty one (62%) of the isolates were resistant to ceporex, 32(64%) and 37(74%) were resistant to ciprofloxacin and tarivid, respectively, 22(44%) and 44(88%) were resistant to peflacine and nalidixic acid, respectively (Table 1 and 2). Fifteen isolates from the different sites were uniformly

resistant to the quinolones (Table 3). The number of isolates from different sites and the percentage resistant to each antibiotic is summarized in Table 4.


Table 1:

Antibiogram of Staphylococcus aureus isolates from Okigwe to quinolones

Image for - Quinolone Resistant Staphylococcus aureus in Okigwe, Imo State Nigeria

R = Resistant, I = Intermediate, S = Sensitivity, <12 mm = Resistant, 12-15 = Intermediate >16 mm sensitive


Table 2:

Number and percentage of Staphylococcus aureus isolates that were resistant, sensitive or intermediate zone to the quinolones

Image for - Quinolone Resistant Staphylococcus aureus in Okigwe, Imo State Nigeria

N = 50, Numbers in bracket are in percentages


Table 3:

Profile of isolates (Staphylococcus aureus) uniformly resistant to the 5 quinolones

Image for - Quinolone Resistant Staphylococcus aureus in Okigwe, Imo State Nigeria

Table 4:

Number of isolates from different sources sensitive and resistant to quinolones

Image for - Quinolone Resistant Staphylococcus aureus in Okigwe, Imo State Nigeria

S = Sensitivity (> 12 mm), R = Resistant (< 6 mm)

DISCUSSION

The high rate of isolation of Staphylococcus aureus in this study (71.42%) is not surprising because S. aureus is a normal microbial flora of humans and can therefore easily gain entry and cause infections as opportunistic pathogen. This high rate of isolation agrees with the finding of Chigbu and Ezeronye (2003). The high rate of resistance of Staphylococcus aureus to nalidixic acid has been reported by other researchers (Chigbu and Ezeronye, 2003). Nalixidic acid is one of the first generation quinolones, which has been on the pharmaceutical shops counter without regulation. People abuse the drug, selecting nalixidate resistant S. aureus. More so, nalidixic acid is a drug used against gram-negative organisms where as S. aureus is gram positive.

The low rate of resistance to peflacine (44%) coupled with other quinolones used for this study could be due to the fact that peflacine has not been long on the Nigerian market unlike the other quinolones that have been in the Nigerian market for some time now. The resistant rate of 64% reported for Ciprofloxacin in this work is similar to that reported by Buck et al. (2005), who reported 65% Ciprofloxacin resistant in Staphylococcus aureus in Minnesota, USA. The work suggested that ciprofloxacin resistant in Minnesota was a result of the use of ciprofloxacin in the treatment of bacterial diseases of poultry. Fluoroquinolones resistance in Okigwe could likely be as a result of drug abuse. The regulation of antimicrobial agents administration is not effective in Nigeria especially with the limited health care facilities available and the high cost of health care delivery. Moreso in the rural communities drugs are easily procured across the counter.

Quinolones resistance by Gram negative and Gram positive cocci other than Staphylococcus has been reported in Taiwan and Alabama on Salmonella and Streptococcus pneumonia (Crystal et al., 2005; Yan et al., 2005) and was attributed to the use of quinolone related compounds in the manufacture of antiseptic soap but in developing countries, the major problem is drug abuse.

Staphylococcus aureus isolates used in this study were most susceptible to peflacine (88%). This suggests that Staphylococcus aureus isolates have not acquired resistance at the rate at which they acquire resistance to other quinolones. This finding agrees with that of Chigbu and Ezeronye (2003) who also reported low resistance to peflacine in S. aureus isolates from both hospital and non hospital subjects in Umuahia Abia State, Nigeria.

The higher prevalence of quinolone resistance S. aureus isolates in Okigwe could be due to widespread indiscriminate use of antimicrobial agents. Rational drug use is important and should be emphasized in the formulation and implementation of a national drug policy by the government. Public health enlightenment on the use of drugs and drug abuse is necessary.

REFERENCES

1:  Atkinson, B.A. and V. Lorian, 1984. Antimicrobial agent susceptibility patterns of bacteria in hospitals from 1971-1982. J. Clin. Microbiol., 20: 791-795.

2:  Aubry-Damon, H., C.J. Soussy and P. Courvallin, 1998. Characterization of mutation in the mob gene that confer rifampicin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother., 42: 2950-2954.

3:  Borehourding, S.M., R. Steven, R.A. Nicholas, C.R. Corley and T. Selt, 1996. Quinolones, a practical review of clinical uses, dosing, consideration and drug interaction. J. Fam. Pract., 42: 65-78.

4:  Buck, J.M., K. Cosmo-Sabetti, K. Harrimen, R.N. Danila, D.J. Boxrud, A. Glenmen and R. Lynfield, 2005. Community associated methicillin resistant Staphylococcus aureus, Minnesota, 2000-2003. Emerg. Infect. Dis., 11: 1532-1538.
Direct Link  |  

5:  Burnett, G.W., W.S. Henry and S.G. Schuster, 1990. Staphylococcus and Staphylococcal Infections Oral Microbiology. 1st Edn., Williams and Wilkins, Philadelphia, pp: 405-416

6:  Cheesbrough, M., 2002. District Laboratory Practice for Tropical Countries Part 2. Cambridge University Press, Cambridge, UK., pp: 157-105

7:  Chigbu, C.O. and O.U. Ezeronye, 2003. Antibiotic resistant Staphylococcus aureus in Abia State of Nigeria. Afr. J. Biotechnol., 2: 374-378.
Direct Link  |  

8:  Crystal, N.J., D.E. Briles, W.H. Benjamin, S.K. Hollinshead and K.B. Braithwaite, 2005. Relative fitness by fluoroquinolone resistant Streptococcus pneumoniae. Emerg. Infect. Dis., 11: 814-820.
Direct Link  |  

9:  Doig, C.M., 1981. Nasal carriage of Staphylococcus in a general Surgical Unit. Br. J. Sur., 58: 113-113.

10:  Levy, S., 1998. The Antibiotic Paradox: How Miracle Drugs are Destroying the Miracle. Plenum Publishers, London, pp: 1-11

11:  Maple, P.A., J. Hamilton-Miller and W. Barunlitt, 1989. Worldwide antibiotics resistance in Methicillin resistant Staphylococcus aureus. Lancet, 1: 539-540.

12:  Neihart, R.E., J.S. Freid and G.R. Hodges, 1988. Coagulase-positive Staphylococci. South Med. J., 81: 491-500.

13:  Nnochiri, R., 1973. The changing patterns of antobiotic resistance and pathogenic bacterial isolates as indicators of drug abuse in middle Africa. Proceedings of the 1973 Annual Scientific Conference of East Africa Medical Research Council.

14:  Paul, M.O., D.A. Aderibigbe, C.Z. Sule and A.A. Lami-Kenra, 1982. Antimicrobial sensitivity pattern of hospital and non hospital strains of Staphylococcus aureus isolated from nasal carrier. J. Hyg., 89: 253-260.

15:  Prescott, L.M., J.P. Harley and D.A. Klein, 1996. Microbiology. 3rd Edn., McGraw Hill, New York

16:  Trucksis, M., D.C. Hooper and J.S. Wolfson, 1991. Emerging resistance to Fluoroquinolones in Staphylococci: An alert. Ann. Int. Med., 114: 424-426.
Direct Link  |  

17:  Uaboi-Egbenni, P.O., 2003. Incidence of Staphylococcus aureus among healthy humans in Lagos and its environs. Nig. J. Microbiol., 17: 162-172.

18:  Yan, J.J., C.S. Chiou, T.C. Yang, S.H. Fsai and J.J. Jong-Wu, 2005. Cephalosporin and ciprofloxacin resistance in Salmonella, Taiwan. Emerg. Infect. Dis., 11: 947-955.
Direct Link  |  

©  2022 Science Alert. All Rights Reserved