Ayman EL-Meghawry EL-Kenawy
Institute of Genetic Engineering and Biotechnology, Menoufiya University, Egypt
Attalla Farag El-kott
Department of Zoology, Faculty of Science, Fayoum University, Egypt
Mashael Mohammed Bin-Meferij
Department of Zoology, Girls College of Education, Scientific Sections, Riyadh, K S.A
Ezz Mohie El Gamal
Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Egypt
ABSTRACT
The aim of the present study was analyzed the expression of epidermal growth factor receptor (EGFR), matrix metalloproteinase-2 and 9 for evaluating their value as a prognostic markers in transitional bladder cancer specimens. The EGFr, MMP-2 and MMP-9 proteins were assayed semiquantitatively in 85 transitional bladder carcinomas of varying grade and stage. Specific monoclonal antibodies for EGFr, MMP-2 and MMP-9 were used to detect the antigens by immunohistochemical staining of paraffin-embedded tissue sections. Of the 85 specimens, 38 (44.7%) overexpressed EGFr, 38 (41.2%) MMP-2 and 38 (44.7%) MMP-9 proteins when three observers scored stained sections independently. The 5-year disease-specific survival was significantly with EGFr, MMP-2, MMP-9, (p<10-6 for all three markers). In multivariate analysis, the three markers remained the prognositic in bladder cancer and the more significant was EGFr. The results of this study demonstrated that EGFr, MMP-2, MMP-9 proteins overexpression may be an independent prognostic biomarker for bladder cancer.
PDF References
How to cite this article
Ayman EL-Meghawry EL-Kenawy, Attalla Farag El-kott, Mashael Mohammed Bin-Meferij and Ezz Mohie El Gamal, 2006. Expressions of Epidermal Growth Factor Receptor, Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 in Bladder Carcinoma. Journal of Biological Sciences, 6: 911-915.
DOI: 10.3923/jbs.2006.911.915
URL: https://scialert.net/abstract/?doi=jbs.2006.911.915
DOI: 10.3923/jbs.2006.911.915
URL: https://scialert.net/abstract/?doi=jbs.2006.911.915
REFERENCES
- Colquhoun, A.J. and J.K. Mellon, 2002. Epidermal growth factor receptor and bladder cancer. Postgrad Med. J., 78: 584-589.
CrossRefDirect Link - Gerhards, S., K. Jung and F. Koening, 2001. Excretion of matrix metalloproteinases 2 and 9 in urine is associated with high stage and grade of bladder carcinoma. Urology, 57: 675-679.
Direct Link - Grignon, D.J., W. Sakr and M. Toth, 1996. High levels of tissue inhibitor of metalloproteinase-2 (TIMP-2) expression are associated with poor outcome in invasive bladder cancer. Cancer Res., 56: 1654-1659.
Direct Link - Hsu, S.M., L. Raine and H. Fanger, 1981. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem., 29: 557-580.
CrossRefPubMedDirect Link - Inoue, K., M. Kamada and J.W. Salton, 2002. The prognostic alue of angiogenesis and metastasis-related genes for progression f transitional cell carcinoma of the renal pelvis and ureter. Lin Cancer Res., 8: 1863-1870.
Direct Link - Nakanishi, K., T. Kawai and H. Sato, 2000. Expression of matrix metalloproteinase-2 (MMP-2) and of membrane-type-1 matrix metalloproteinase (MT1-MMP) in transitional cell carcinoma of the upper urinary tract. Hum. Pathol., 31: 193-200.
PubMedDirect Link - Nicholson, R.I., J.M.W. Gee and M.E. Harper, 2001. EGFR and cancer prognosis. Eur. J. Can., 37: 9-15.
Direct Link - Penault-Llorca, F., X. Durando and J.O. Bay, 2003. Prognostic value of epidermal growth factor receptor. Bull. Cancer, 90: S192-S196.
PubMedDirect Link - Yasuyoshi, M., K. Shigeru and N. Koichiro, 2004. Expression of metalloproteinase-2, metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in transitional cell carcinoma of upper urinary tract: Correlation with tumor stage and survival. Urology, 63: 602-608.
PubMedDirect Link - Kaplan, E.L. and P. Meier, 1958. Nonparametric estimation from incomplete observations. J. Am. Stat. Assoc., 53: 457-481.
CrossRefDirect Link