Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Research Article
 

Optimizing Carrot Hairy Root Production for Monoxenic Culture of Arbuscular Mycorrhizal Fungi in Iran



Y.R. Danesh , E. Mohammadi Goltapeh , A. Alizadeh and M. Modarres Sanavy
 
ABSTRACT

This research describes the optimized method of carrot hairy roots production for monoxenic culture of Arbuscular Mycorrhizal Fungi (AMF) in Iran. Inoculation of mature carrot root sections was carried out using four isolates of Agrobacterium rhizogenes as well as a mixture of isolates that had been grown in three culture media (LB, YMA and modified medium) with different incubation times (24 and 36 h) before inoculation. Three methods of carrot hairy root initiation were compared in an attempt to optimize hairy root production in terms of the number of root initiations and the health (lack of rotting) of the explants. Results showed that most treatments resulted in hairy root formation, although not equally so. It was shown that the mixture of bacterial strains from three culture media increased the frequency of bacterial colonies with a good transformation characteristic. Also, incubation of inoculated carrot discs for 48-72h in darkness provided a suitable condition for bacterial strains to insert their copies of Ri T-DNA. The source of the carrots was one of the most important factors. With mature, freshly harvested carrots showing better hairy root production. Dissection of the cortex to expose the cambium had a positive effect on hairy root initiation. Increasing bacterial strains incubation time from 24 to 36 h improved transformation efficiency in all treatments but not in method III. Results showed that the best method for initiation of hairy root induction in carrots especially in Iran is the method I.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

Y.R. Danesh , E. Mohammadi Goltapeh , A. Alizadeh and M. Modarres Sanavy , 2006. Optimizing Carrot Hairy Root Production for Monoxenic Culture of Arbuscular Mycorrhizal Fungi in Iran. Journal of Biological Sciences, 6: 87-91.

DOI: 10.3923/jbs.2006.87.91

URL: https://scialert.net/abstract/?doi=jbs.2006.87.91

REFERENCES
Becard, G. and J.A. Fortin, 1988. Early events of vesicular arbuscular mycorrhiza formation on Ri T DNA transformed roots. New Phytol., 108: 211-218.

Becard, G. and Y. Piche, 1992. Establishment of Vesicular Arbuscular Mycorrhiza in Root Organ Culture Review and Proposed Methodology. In: Techniques for the Study of Mycorrhiza, Norris, J., D. Read and A. Varma (Eds.). Academic Press, New York, pp: 89-108.

Butcher, D.N. and H.E. Street, 1964. Excised root culture. Bot. Rev., 30: 513-586.

Butcher, D.N., 1980. The Culture of Isolated Roots. In: Tissue Culture Methods for Plant Pathologists, Ingram, D.S. and J.P. Helgelson (Eds.). Blackwell Scientific Publications, Oxford, pp: 13-17.

Christey, M.C. and R.H. Braun, 2005. Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation. Methods Mol. Biol., 286: 47-60.
PubMed  |  Direct Link  |  

Dhakulkar, S., T.R. Ganapathi, S. Bhargava and V.A. Bapat, 2005. Induction of hairy roots in Gmelina arborea Roxb. and production of verbascoside in hairy roots. Plant Sci., 169: 812-818.
CrossRef  |  

Diop, T.A., G. Becard and Y. Piche, 1992. Long-term in vitro culture of an endomycorrhizal fungus, Gigaspora margarita, on Ri T-DNA transformed root of carrot. Symbiosis, 12: 249-259.
Direct Link  |  

Fu, C., D. Zhao, X. Xue, Z. Jin and F.S. Ma, 2005. Transformation of Saussurea involucrate by Agrobacterium rhizogenes hairy root induction and syringin production. Process Biochem., 40: 789-3794.
Direct Link  |  

Mugnier, J. and B. Mosse, 1987. Vesicular arbuscular infections in Ri T DNA transformed roots grown axenically. Phytopathology, 77: 1045-1050.

Mugnier, J., 1988. Establishment of new axenic hairy root lines by inoculation with Agrobacterium rhizogenes. Plant Cell Rep., 7: 9-12.
CrossRef  |  Direct Link  |  

Riker, A.J., W.M. Banfield, W.H. Wright, G.W. Keitt and H.E. Sagen, 1930. Studies on infectious hairy root of nursery apple trees. J. Agric. Res., 41: 507-540.

Ryder, M.H., M.E. Tate and A. Kerr, 1985. Virulence properties of strains of Agrobacterium on the apical and basal surfaces of carrot root discs. Plant Physiol., 77: 215-221.
Direct Link  |  

SAS. 1992. The SAS System for Windows 3.10, Release 6.08. SAS Institute Inc., Cary, North Carolina.

Smith, S. and S. Dickson, 1997. VA Mycorrhizas Basic Research Techniques. Cooperative Research Center for Soil and Land Management, Adelaide, Australia, pp: 39.

Tepfer, D., 1989. Ri T DNA from Agrobacterium rhizogenes a Source of Genes Having Applications in Rhizosphere Biology and Plant Development Ecology and Evolution. In: Plant Microbe Interactions, Kosuge, T. and E.W. Nester (Eds.). McGraw-Hill Publishing, New York, pp: 294-342.

Tepfer, D.A. and J. Tempe, 1981. Production dagropine par des raciness formees sous laction d=Agrobacterium rhizogenes souche A4. CR Acad. Sci. Paris, 292: 153-156.

Velde, W.V., J. Mergeay, M. Holsters and S. Goormachtig, 2003. Agrobacterium rhizogenes mediated transformation of Sesbania rostrata. Plant Sci., 165: 1281-1288.
CrossRef  |  

White, P.R., 1943. A Handbook of Plant Tissue Culture. Jacques Cattell Press, Lancaster, PA., USA.

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract