Durdi Qujeq
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, 143 005, Punjab, India
Rahim Jourjani Daz
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, 143 005, Punjab, India
ABSTRACT
The aim of this study was to determine the effects of thioperamide on the total brain histamine content and histidine decarboxylase activity of mouse brain. Mice (n = 24) was used in this study. The animals were housed six per cage, water and food were allowed. All experimented manipulations were carried out with the animals under ether inhalation anesthesia. Mice were injected with 20 mg kg-1 of thioperamide 30 min and 5 h after IP injection of thioperamide and brains were removed. The brain homogenate was centrifuged and supernatant was obtained. Aliquots were removed for determination of histamine content and HD activity. The florescence was measured at 440 nm with excitation at 360 nm. The HD activity of brain was higher of treated mice (63.8±4.6 nmol mg/protein/min) than in control group (49.1±3.4 nmol mg/protein/min). Treated mice had a statistically significant increase in mean brain histamine level (243.7±7.9 nmol mg/protein) when compared with control group (197.3±8.2 nmol mg/protein). The increase in the histamine content and HD activity in the brain after injection of thioperamide in the brain, may contribute of the stimulate of histidine decarboxylase enzyme by thioperamide.
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How to cite this article
Durdi Qujeq and Rahim Jourjani Daz, 2006. The Effects of Thioperamide on the Histamine Level and Histidine Decarboxylase Activity of Mouse Brain. Journal of Biological Sciences, 6: 61-64.
DOI: 10.3923/jbs.2006.61.64
URL: https://scialert.net/abstract/?doi=jbs.2006.61.64
DOI: 10.3923/jbs.2006.61.64
URL: https://scialert.net/abstract/?doi=jbs.2006.61.64
REFERENCES
- Imaizumi, M. and K. Onodera, 1993. The behavioral and biochemical effects of thioperamide a histamine H3 receptor antagonist in a light dark test measuring anxiety in mice. Life Sci., 53: 1675-1683.
PubMed - Yamamoto, Y., T. Mochizuki, K.O. Mochizuki, A. Uno and A. Yamatodani, 1997. Thioperamide a histamine H3 receptor antagonist increases GABA release from the rat hypothalamus. Methods Find Exp. Clin. Pharmacol., 19: 289-298.
PubMed - Lapham, J. and G.J. Kilpatrick, 1994. Thioperamide the selective histamine H3 receptorantagonist attenuates stimulant induced locomotor activity in the mouse. Eur. J. Pharmacol., 259: 107-114.
PubMed - Detzner, M., M. Kathmann and E. Schlicker, 1994. Time course of the effects of histamine thioperamide and EEDQ on H3 receptors in the mouse brain. Agents Actions, 41: 66-67.
CrossRef - Sakurai, E., E. Gunji, Y. Iizuka, N. Hikichi, K. Maeyama and T. Watanabe, 1994. The disposition of thioperamide a histamine H3 receptor antagonist in rats. J. Pharm. Pharmacol., 46: 209-212.
PubMed - Acuna, Y., Y. Mathison, H.A. Campos and A. Israel, 1998. Thioperamide a histamine H3 receptor blocker facilitates vasopressor response to footshocks. Inflamm. Res., 47: 109-114.
PubMed - Meguro, K., K. Yanai, N. Sakai, E. Sakurai, K. Maeyama, H. Sasaki and T. Watanabe, 1995. Effects of thioperamide, a histamine H3 antagonist on the step through passive avoidance response and histidine decarboxylase activity in senescence accelerated mice. Pharmacol. Biochem. Behav., 50: 321-3255.
Direct Link - Sakai, N., A. Sakurai, E. Sakurai, K. Yanai, K. Maeyama and T. Watanabe, 1992. Effects of the histamine H3 receptor ligands thioperamide and (R) α methylhistamine on histidine decarboxylase activity of mouse brain. Biochem. Biophys. Res. Commun., 185: 121-126.
Direct Link - Vohora, D., S.N. Pal and K.K. Pillai, 2000. Thioperamide a selective histamine H3 receptor antagonist protects against PTZ induced seizures in mice. Life Sci., 66: 297-301.
CrossRef - Rodrigues, A.A, R. Leurs, T.S. Wu, G.D. Prell and C. Foged, 1996. Timmerman H [3H] thioperamide as a radioligand for the histamine H3 receptor in rat cerebral cortex. Br. J. Pharmacol., 118: 2045-2052.
Direct Link - Itoh, E., M. Fujimiya and A. Inui, 1998. Thioperamide a histamine H3 receptor antagonist suppresses NPY but not dynorphin a induced feeding in rats. Regul. Pept., 6: 373-376.
Direct Link - Itoh, E., M. Fujimiya and A. Inui, 1999. Thioperamide a histamine H3 receptor antagonist powerfully suppresses peptide YY induced food intake in rats. Biol. Psychiatry, 45: 475-481.
Direct Link - Oishi, R., 1988. Turnover of brain histamine and its changes by various drugs. Nippon Yakurigaku Zasshi, 92: 271-281.
PubMed - Arrang, J.M., M. Garbarg and C. Schwartz, 1983. Auto inhibition of brain histamine release mediated by a novel class(H3) of histamine receptor. Nature, 302: 832-837.
CrossRef - Watanabe, T., H. Nakamura, L.Y. Liang, A. Yamatodani and H. Wada, 1979. Partial purification and characterization of L histidine decarboxylase from fetal rats. Biochemi. Pharmacol., 28: 1149-1155.
PubMed - Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
CrossRefPubMedDirect Link