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The Effects of Thioperamide on the Histamine Level and Histidine Decarboxylase Activity of Mouse Brain

Durdi Qujeq and Rahim Jourjani Daz

The aim of this study was to determine the effects of thioperamide on the total brain histamine content and histidine decarboxylase activity of mouse brain. Mice (n = 24) was used in this study. The animals were housed six per cage, water and food were allowed. All experimented manipulations were carried out with the animals under ether inhalation anesthesia. Mice were injected with 20 mg kg-1 of thioperamide 30 min and 5 h after IP injection of thioperamide and brains were removed. The brain homogenate was centrifuged and supernatant was obtained. Aliquots were removed for determination of histamine content and HD activity. The florescence was measured at 440 nm with excitation at 360 nm. The HD activity of brain was higher of treated mice (63.8±4.6 nmol mg/protein/min) than in control group (49.1±3.4 nmol mg/protein/min). Treated mice had a statistically significant increase in mean brain histamine level (243.7±7.9 nmol mg/protein) when compared with control group (197.3±8.2 nmol mg/protein). The increase in the histamine content and HD activity in the brain after injection of thioperamide in the brain, may contribute of the stimulate of histidine decarboxylase enzyme by thioperamide.

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  How to cite this article:

Durdi Qujeq and Rahim Jourjani Daz , 2006. The Effects of Thioperamide on the Histamine Level and Histidine Decarboxylase Activity of Mouse Brain. Journal of Biological Sciences, 6: 61-64.

DOI: 10.3923/jbs.2006.61.64


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