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A Possible Role for Methyl Jasmonate in Effecting Superoxide Dismutase and Catalase Activities under PQ-induced Oxidative Stress in Maize Seedlings

Majid Nojavan-Asghari and Akbar Norastehnia

In the present study the effects of different concentrations of MeJA on the activities of SOD and CAT in roots and shoots of corn seedlings were studied in two parts. In the first part, the plants were pretreated with 0, 5, 10, 20, 50 and 100 μmol methyl jasmonate 24 h before PQ application as an inducer of oxidative stress agent. In the second part, the 0, 20, 50 and 100 μmol concentrations of MeJA were used after PQ imposed oxidative stress. Subsequently, the intensity of enzymatic activities in roots and shoots were separately measured. The certainty of oxidative stress imposition by PQ was investigated by measuring the MDA levels as a product of lipid peroxidation and indicator of oxidative stress. Reducing the levels of lipid peroxidation to the control level was accomplished by increasing the activity of SOD and CAT through changes in concentration and time of application of MeJA in roots and shoots. In this relation, the 50 and 100 μmol concentrations of MeJA could decrease the lipid peroxidation with highest efficiency and meaningfully in roots and shoots as compared with controls.

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  How to cite this article:

Majid Nojavan-Asghari and Akbar Norastehnia , 2006. A Possible Role for Methyl Jasmonate in Effecting Superoxide Dismutase and Catalase Activities under PQ-induced Oxidative Stress in Maize Seedlings . Journal of Biological Sciences, 6: 55-60.

DOI: 10.3923/jbs.2006.55.60


Asada, K. and M. Takahashi, 1987. Production and Scavenging o f Active Oxygen In Photosynthesis. In: Photoinhibition, Kyle, D.J., C.D. Osmond and C.J. Arntzen (Eds.). Elsevier Sciece Pulishers, Amsterdam, pp: 227-287.

Ashton, F.M. and A.S. Crafts, 1981. Mode of Action of Herbicides. Wiley InterScience, New York, pp: 166-175.

Babbs, C.F., J.A. Pham and R.C. Coolbaugh, 1989. Lethal hydroxyl radical production in paraquat treated plants. Plant Physiol., 90: 1267-1270.

Baldwin, I.T., Z.P. Zhang, N. Diab, T.E. Ohnmeiss, E.S. McCloud, G.Y. Lynds and E.A. Schmelz, 1997. Quantification, correlations and manipulations of wound-induced changes in jasmonic acid and nicotine in Nicotiana sylvestris. Planta, 201: 397-404.
CrossRef  |  

Bueno, P. and L.A.D. Rio, 1992. Purification and properties of glyoxysomal cuprozinc superoxide dismutase from watermelon cotyledons. Plant Physiol., 98: 331-336.

Choi, D.G., N.H. Yoo, C.Y. Yu, B.D.L. Reyes and S.J. Yun, 2004. The activities of antioxidant enzymes in response to oxidative stress and hormones in paraquat tolerant Rehmannia glotinosa plants. J. Biochem. Mol. Biol., 37: 618-624.

Creelman, R.A. and J.E. Mullet, 1995. Jasmonic acid distribution and action in plants regulation during development and response to biotic and abiotic stress. Proc. Natl. Acad. Sci. USA., 92: 4114-4119.

Creelman, R.A. and J.E. Mullet, 1997. Biosynthesis and action of jasmonates in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol., 48: 355-381.
CrossRef  |  PubMed  |  Direct Link  |  

Del-Rio, L.A., L.M. Saudalio, J.M. Palma, P. Bueno and F.J. Corpas, 1992. Metabolism of oxygen radicals in peroxisomes and cellular implications. Free Radical Med. Biol., 13: 557-580.
Direct Link  |  

Dhindsa, R.S., P. Plumb-Dhindsa and T.A. Thorpe, 1981. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. J. Exp. Bot., 32: 93-101.
CrossRef  |  Direct Link  |  

Dodge, A., 1994. Heribcide Action and Effects on Detoxification Process. In: Causes of Photooxidative Stress and Amelioration of Defence System in Plants, Foyer, C.H. and P.M. Mullineaux (Eds.). CRC Press, Boca Raton, FL., pp: 219-236.

Donahue, J.L., C.M. Okpodu, C.L. Cramer, E.A. Grabau and R.G. Alscher, 1997. Responses of antioxidants to paraquat in pea leaves. Plant Physiol., 113: 249-257.
Direct Link  |  

Farmer, E.E. and C.A. Rayan, 1992. Octadecanoid precursors of jasmonic acid activate the synthesis of wound in ducible proteinase inhibitors. Plant Cell, 4: 129-134.

Farmer, E.E., H. Weber and S. Vollenweider, 1998. Fatty acid signaling in Arabidopsis. Planta, 206: 167-174.
CrossRef  |  Direct Link  |  

Foyer, C.H., K. Lelandais and K.J. Kunert, 1994. Photooxidative stress in plants. Physiol. Plant., 92: 696-717.
CrossRef  |  

Foyer, C.H., P. Descourvieres and K.J. Kunert, 1994. Protection against oxygen radicals an important defence mechanism studied in transgenic plants. Plant Cell Environ., 17: 507-523.

Franceschi, V.R. and H.D. Grimes, 1991. Induction of soybean vegetative storage proteins and anthocyanins by low level atmospheric methyl jasmonate. Proc. Natl. Acad. Sci. USA., 88: 6745-6749.
Direct Link  |  

Gundlach, H., M.J. Muller, M.J. Kutchan and M.H. Zenk, 1992. Ja is a signal transducer elicitor-induced plant cell cultures. Proc. Natl. Acad. Sci., USA., 89: 2389-2393.
PubMed  |  

Hassan, H.M., 1989. Microbial superoxide dismutase. Adv. Genet., 26: 65-97.

Hauptmann, N. and E. Cadenas, 1997. The Oxygen Paradox Biochemistry of Active Oxygen. In: Oxidative Stress and the Molecular Biology of Antioxidant Defence, Sandalios, J.G. (Ed.). Cold Spring Harbor Laboratory Press, New York, pp: 1-20.

Heath, R.L. and L. Packer, 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys., 125: 189-198.
CrossRef  |  PubMed  |  Direct Link  |  

Iannelli, M.A., F.V. Breusegem, M.V. Montagu, D. Inze and A. Massacci, 1999. Tolerance to low temperature and paraquat-mediated oxidative stress in two maize geotypes. J. Exp. Bot., 50: 523-532.

Kanematsu, S. and K. Asada, 1990. Characteristic amino acid sequence of chloroplast and cytosol enzymes of Cu Zn superoxide dismutase in spinach rice and horsetail. Plant Cell Physiol., 31: 99-112.

Kang, H.M. and M.E. Salveit, 2002. Antioxidant enzymes and DPPH radical scavenging activity in chilled and heat shocked rice (Oryza sativa L.) seedling radicles. J. Agric. Food Chem., 50: 513-518.

Kim, S.R., J.L. Choi, M.A. Costa and G. An, 1992. Identification of G box sequence as an assential element for methyl jasmonate response of potato proteinase inhibitor II promoter. Plant Physiol., 99: 627-631.
Direct Link  |  

Levine, R.L., D. Garland, C.N. Oliver, A. Amici and I. Climent et al., 1990. Determination of carbonyl content in oxidatively modified proteins. Meth. Enzymol., 186: 464-478.
CrossRef  |  PubMed  |  Direct Link  |  

Mackerness, S.A.H., S.L. Surplus, P. Blake, C.F. John, V Buchanan-Wollaston, B.R. Jordan and B. Thomas, 1999. Ultraviolet B induced stress and changes in gene expression in Arabidopsis thaliana: Role of signaling pathways controlled by jasmonic acid ethylene and reactive oxygen species. Plant Cell Environ., 22: 1413-1423.
CrossRef  |  

Maehly, A.C. and B. Chance, 1959. The Assay of Catalase and Peroxidase. In: Methods of Biochemical Analysis, Glik, D. (Ed.). Interscience Publishers, New York, pp: 357-425.

Mason, H.S., D.B. de Wald and J.E. Mullet, 1993. Identification of a methyl jasmonate responsive domain in the Soybean sp. Promoter. Plant Cell, 5: 241-251.

Melhorn, H., 1990. Ethylen promoted ascorate peroxidase activity protects plants against hydrogen peroxide ozone and paraquat. Plant Cell Environ., 13: 971-976.

Mihara, M., M. Uchiyama and K. Fukazawa, 1980. Thiobarbituric acid value on fresh homogenate of rat as a parameter of lipid peroxidation in aging, CCl4 intoxication and vitamin E deficiency. Biochem. Med., 23: 302-311.

Mittler, R. and B.A. Zilinskas, 1992. Molecular cloning and characterization of a gene encoding pea cytosolic ascorate peroxidase. J. Biol. Chem., 267: 21802-21807.

Palma, J.M., L.M. Sandalio and L.A. Del Rio, 1986. Manganese superoxide dismutase in higher plant chloroplasts. J. Plant Physiol., 125: 427-439.

Popova, L., E. Ananieva, V. Hristova, K Christov, K. Georgieva, V. Alexieva and Z. Stoinova, 2003. Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulg. J. Plant Physiol., pp: 133-152.

Prochazkova, D., R.K. Sairam, G.C. Srivastava and D.V. Singh, 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci., 161: 765-771.
CrossRef  |  Direct Link  |  

Reinbothe, S., C. Reinbothe, J. Lehmann and B. Parthier, 1992. Differential accumulation of methyl jasmonate induced mRNAs in response to abscisic acid and desiccation in barley (Hordeum vulgare). Physiol. Plant., 86: 49-56.
Direct Link  |  

Sandalio, L.M. and L.A.D. Rio, 1988. Intraorganellar distribution of superoxide dismutase in plant peroxisomes. Plant Physiol., 88: 1215-1218.

Scandalios, J.G., W.F. Tog and D.G. Roupakias, 1980. Cat3 a third gene locus coding for a tissue specific catalase in maize. Mol. Gen. Genet., 179: 33-41.

Tsonev, T.D., G.N. Lazova, Z.G. Stoinova and L.P. Popova, 1998. A possible role for jasmonic acid in adaptation of barley seedlings to salinity stress. J. Plant Growth Regul., 17: 153-159.
CrossRef  |  

Wilen, R.W., E.E. Bruce and L.V. Gusta, 1994. Interaction of abscisic acid and jasmonic acid on the inhibition of seed germination and the induction of freezing tolerance. Can. J. Bot., 72: 1009-1017.
Direct Link  |  

Willekens, H., D. Inze, M.V. Montagu and W.V. Camp, 1995. Catalases in plants. Mol. Breed., 1: 207-228.
Direct Link  |  

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