M .M. Camargo de Morais
LaboratOrio de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Cidade Universitaria, Recife, FE, Brazil, CEP 50670-901
S.A. F. Ramos
LaboratOrio de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Cidade Universitaria, Recife, FE, Brazil, CEP 50670-901
M.C. B. Pimentel
Departamento de Bioquimica, Universidade Federal de Pernambuco, Cidade Universitaria, Recife, FE, Brazil, CEP 50670-901
E. H.M. Melo
Departamento de Bioquimica, Universidade Federal de Pernambuco, Cidade Universitaria, Recife, FE, Brazil, CEP 50670-901
M.A. Morais Jr
Departamento de Gen6tica,
Universidade Federal de Pernambuco, Cidade Universitaria, Recife, FE, Brazil, CEP 50670-901
J.F. Kennedy
Chembiotech Laboratories, University of Birmingham Research Park,
Vincent Drive, Birmingham B 15 25Q, UK
J.L. Lima Filho
LaboratOrio de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Cidade Universitaria, Recife, FE, Brazil, CEP 50670-901
ABSTRACT
A Brazilian strain of Penicillium citrinum produced a lipopolysaccharide with emulsifier properties during cultivation on mineral medium, containing ammonium sulfate as nitrogen source, with 1% (v/v) olive oil as the carbon source. The maximal emulsifier production (1.6 U mL-1) was obtained after 60 h of cultivation and the biomass reached 7.5 g L-1 at the end of fermentation. The production yield i.e. the amount the carbon source utilized for product synthesis was 54% and the best emulsifying activity was observed for xylene and diesel oil when compared to other carbohydrates tested. The emulsifier was shown to be stable to a wide range of pH and temperature values and was shown to contain D-galactose, D-glucose and D-xylose (8.2:1.0:5.3) with a total carbohydrate content of 43%. The presence of salts stimulated the emulsification activity, suggesting potential for its application in industrial waste or marine remediation.
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How to cite this article
M .M. Camargo de Morais, S.A. F. Ramos, M.C. B. Pimentel, E. H.M. Melo, M.A. Morais Jr, J.F. Kennedy and J.L. Lima Filho, 2006. Lipopolysaccharide Extracellular Emulsifier Produced by Penicillium citrinum. Journal of Biological Sciences, 6: 511-515.
DOI: 10.3923/jbs.2006.511.515
URL: https://scialert.net/abstract/?doi=jbs.2006.511.515
DOI: 10.3923/jbs.2006.511.515
URL: https://scialert.net/abstract/?doi=jbs.2006.511.515
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