R. Panesar
Department of Biotechnology, Punjabi University, Patiala 147 002, India
P.S. Panesar
Department of Food Technology, Sant Longowal Institute of Engineering and Technology, Longowal 148 1 06, India
R.S. Singh
Department of Biotechnology, Punjabi University, Patiala 147 002, India
J.F. Kennedy
Birmingham Carbohydrate and Protein Technology Group, School of Chemistry, University of Birmingham, Birmingham Bl5 2TT, UK
M. B. Bera
Department of Food Technology, Sant Longowal Institute of Engineering and Technology,Longowal 148 1 06, India
ABSTRACT
The use of Kluyveromyces marxianus NCIM 3465 for the production of ß-D-galactosidase is reported. Experimentation was carried out to optimize the process parameters for maximum enzyme production by varying the parameters such as pH of the medium, temperature, inoculum size, age of inoculum, agitation and incubation time. The maximum enzyme activity was observed with pH 5.0, temperature 30°C, inoculum size 6% (v/v) having 20 h age, under shaking conditions (100 rpm) after 24 h of incubation.
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How to cite this article
R. Panesar, P.S. Panesar, R.S. Singh, J.F. Kennedy and M. B. Bera, 2006. Process Optimization for ß-D-Galactosidase Production Using Yeast Culture. Journal of Biological Sciences, 6: 193-197.
DOI: 10.3923/jbs.2006.193.197
URL: https://scialert.net/abstract/?doi=jbs.2006.193.197
DOI: 10.3923/jbs.2006.193.197
URL: https://scialert.net/abstract/?doi=jbs.2006.193.197
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