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Research Article
 
Propagation of Infectious Bursal Disease Virus (IBDV) in Chicken Embryo Fibroblast Cells



SK. Amir Hossain , Sarder Nasir Uddin , Muhammad Sadiqur Rahman , Abdul Wadud and Muhammad Harunuzzaman Khan
 
ABSTRACT

Infectious bursal disease is an acute, highly contagious viral disease of young chickens caused by a double stranded RNA virus named Infectious Bursal Disease Virus (IBDV). Chicken Embryo Fibroblast (CEF) is chicken embryo derived primary cell culture. The present research was undertaken to study the propagation and observation of cytopathic effect of IBDV in chicken embryo fibroblast cells. Chicken Embryo Fibroblast (CEF) is chicken embryo derived primary cell culture. For this purpose, suspected IBDV isolates were collected from the bursas of dead chicken of a particular flock. Local field IBDV isolates was then inoculated in chicken embryo for slight adaptation by several passages. CEF cells was prepared from 9-10 days chicken embryo and transferred in monolayer culture flasks containing maintenance media with 1-2% heat-inactivated fetal calf serum. Adapted IBDV was then inoculated in CEF cell culture for the purpose of propagation. Characteristics clear and consistent Cytopathic Effects (CPEs) were observed on CEF cell culture after 144 h of incubation following infection of IBDV.

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  How to cite this article:

SK. Amir Hossain , Sarder Nasir Uddin , Muhammad Sadiqur Rahman , Abdul Wadud and Muhammad Harunuzzaman Khan , 2006. Propagation of Infectious Bursal Disease Virus (IBDV) in Chicken Embryo Fibroblast Cells. Journal of Biological Sciences, 6: 146-149.

DOI: 10.3923/jbs.2006.146.149

URL: https://scialert.net/abstract/?doi=jbs.2006.146.149

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