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Studies on Antimicrobial and Antioxidative Substance of Yuzu (Citrus junos hort. ex Tanaka) Seed

Yukiko Nakazono , Yuka Watanabe , Fumio Hashinaga and Kenjiro Tadera

A yuzu (Citrus junos hort. ex Tanaka) seed extract with 80% methanol showed a potent antimicrobial activity against Micrococcus luteus, Staphylococcus aureus, Escherichia coli and Salmonella enteritidis. The antimicrobial substance was purified by adsorption column chromatography and identified as p-methoxycinnamic acid by gas chromatography-mass spectrometry. Minimum inhibitory concentrations of p-methoxycinnamic acid against M. luteus, S. aureus, E. coli and S. enteritidis were 80, 60, 50 and 60 μg mL-1, respectively. In addition, this structure suggested that p-methoxycinnamic acid had the antioxidative activity and we investigated also the antioxidative activity. The antioxidative activity of p-methoxycinnamic acid was about 70-80% of that of BHA, BHT and α-tocopherol.

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Yukiko Nakazono , Yuka Watanabe , Fumio Hashinaga and Kenjiro Tadera , 2006. Studies on Antimicrobial and Antioxidative Substance of Yuzu (Citrus junos hort. ex Tanaka) Seed. Journal of Biological Sciences, 6: 135-139.

DOI: 10.3923/jbs.2006.135.139


Alan, D., 1991. Fruit. Mitchell Beazley Publishers, England, pp: 74.

Ariga, T., H. Hosoyama, S. Tokutake and J. Yamakoshi, 2000. Studies on functional properties of proanthocyanidins and their practical application. Nippon Nogei Kagaku Kaishi, 74: 1-6.
Direct Link  |  

Balasubramanian, N., B. Deepak, P. Devayani, M. Vishnukant and D. Avinash, 2004. Esters, amides and substituted derivatives of cinnamic acid: synthesis, antimicrobial activity and QSAR investigations. Eur. J. Med. Chem., 39: 827-834.
CrossRef  |  PubMed  |  Direct Link  |  

Chuyen, N.V., T. Kurata, H. Kato and M. Fujimaki, 1982. Antimicrobial activity of kumazasa (Sasa albo-marginate). Agric. Biol. Chem., 46: 971-978.
Direct Link  |  

Halliwell, B., 1994. Free radicals and antioxidants. Nutr. Rev., 52: 253-265.

Hocman, G., 1988. Chemoprovention of cancer phenolic antioxidants ( BHT, BHA ). Intl. J. Biochem., 20: 639-651.

Hudson, E.A., P.A. Dinh, T. Kokubun, S. Monigue, J. Simmonds and G. Andreas, 2000. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiol. Biomarkers Prevention, 9: 1163-1170.
Direct Link  |  

Ito, S., 1991. Science of Fruits. Asakura Syoten, Japan, pp: 142-143.

Jayaprakasha, G.K., T. Selvi and K.K. Sakariah, 2003. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Res. Int., 36: 117-122.
CrossRef  |  Direct Link  |  

Kim, J., M.R. Marshall and C.I. Wei, 1995. Antibacterial activity of some essential oil components against five foodborne pathogens. J. Agric. Food Chem., 43: 2839-2845.
CrossRef  |  Direct Link  |  

Kim, S.R. and Y.C. Kim, 2000. Neuroprotective phenylpropanoid esters of rhamnode isolated from roots of Scrophularia buergeriana. Phytochemistry, 54: 503-509.

Kim, S.R., S.Y. Kang, K.Y. Lee, S.H. Kim, G.J. Markelonis, T.H. Oh and Y.C. Kim, 2003. Anti amunestic activity of Ep methoxycinnamic acid from Scrophularia buergeriana. Cornitive Brain Res., 17: 454-461.

Kosuge, K., K. Mitsunaga, K. Koike and T. Ohmoto, 1994. Studies on the constituents of Ailanthus integrifolia. Chem. Pharm., 42: 1669-1671.
Direct Link  |  

Kuo, Y.H., Z.S. Chen and Y.L. Lin, 1996. Chemical components of the leaves of Duranta repens LINN. Chem. Pharm., 44: 429-436.
Direct Link  |  

Lee, E.J., S.R. Kim, J. Kim and Y.C. Kim, 2002. Hepatoprotective phenyl propanoids from Scrophularia buergeriana roots against CCl 4 induced toxicity action mechanism and structure activity relation ship. Planta Medica, 68: 407-411.

Matsusaka, Y., J. Kawabata and T. Kasai, 2003. Antioxidative constituents in avocado (Persea americana Mill.) seeds. Nippon Shokuhin Kagaku Kogaku Kaishi, 50: 550-552.

Miyazawa, M., Y. Okuno, S. Nakamura and H. Kameoka, 1998. Suppression of SOS inducing activity of chemical mutagens by cinnamic acid derivatived from Scrophulia ningpoensis in the Salmonella typhimurium TA 1535/PSK1002 umu test. J. Agric. Food Chem., 46: 904-910.
CrossRef  |  

Nishina, C., N. Enoki, S. Tawata, A. Mori, K. Kobayashi and M. Fukushima, 1987. Antibacterial activity of flavonoids against Staphylococcus epidermidis a skin of bacterium. Agric. Biol. Chem., 51: 139-143.
Direct Link  |  

Omi, K., 1999. The Japanese Specifications and Standards for Food Additives. Japan Food Additives Association Press, Japan, pp: 207.

Ping, C.K., C.C. Chao, S.S. Li, Y.L. Chia and S.W. Tian et al., 2002. Non alkaloidal constituents from the stem of Ficus septica. J. Chin. Chem. Soc., 49: 113-116.
Direct Link  |  

Shirasaka, N., A. Kuramatsu, N. Kondo, S. Kondo and H. Yoshizumi et al., 1999. Isolation and characterization of antioxidative compounds from ume ( Prunus mume ) liqueur. Nippon Shokuhin Kagaku Kogaku Kaishi, 46: 792-798.
Direct Link  |  

Sirichai, A., S. Kasem, R. Sophon, P. Amorn and Y. Sirintorn et al., 2004. Structure activity relationships of trans cinnamic acid derivatives on α-glucosidase inhibition. Bioorg. Med. Chem. Lett., 14: 2893-2896.
Direct Link  |  

Tesaki, S., S. Tanabe, M. Moriyama, E. Fukushi, J. Kuwabara and M. Watanabe, 1999. Isolation and identification of an antibacterial compound from grape and its application to foods. Nippon Nogei Kagaku Kaishi, 73: 125-128.
Direct Link  |  

Wattenburg, L.W., 1978. Inhibition of chemical carcinogenesis. J. Natl. Cancer Inst., 60: 11-18.

Wattenburg, L.W., 1972. Inhibition of carcinogenic and toxic effects of polycyclic hydrocarbons by phenolic antioxidants and ethoxyguin. J. Natl. Cancer Inst., 48: 1425-1430.

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