ABSTRACT
The objective of this study was to determine the influence of different plant hormones on gossypol accumulation in callus culture and morphogenesis of cotton (G.hirsutum.L). Conditions have been described for in vitro culturing of tissues from the seeds of two different types of cotton (glanded and glandless). The callus cultures were initiated using defined levels of Kinetin (Kn) and 6-Benzylaminopurine (BAP) and 2.4-dichlorophenoxyacetic acid (2:4-D) and 1-naphthaleneacetic Acid (NAA) and were then transferred on the same media and on media with no hormones also. The gossypol content of each callus cultures and different parts (root, stem and leaf) of aseptically grown (7 to 10 days old) seedlings and seeds was determined using HPLC for cotton plants. The different levels of auxin and cytokinin in the culture media led to differentiation and increased the accumulation of gossypol.
PDF References Citation
How to cite this article
R. Baksha, G.T. Mavlanov, G.B. Nasirova and S.A. Djataev, 2006. Gossypol Accumulation and Morphogenesis in Cotton (G. hirsutum L.) Callus Culture. Journal of Biological Sciences, 6: 1126-1129.
DOI: 10.3923/jbs.2006.1126.1129
URL: https://scialert.net/abstract/?doi=jbs.2006.1126.1129
DOI: 10.3923/jbs.2006.1126.1129
URL: https://scialert.net/abstract/?doi=jbs.2006.1126.1129
REFERENCES
- Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRefDirect Link - Collinge, M.A. and M.M. Yeoman, 1986. The Relationship Between Tropane Alkaloid Production and Structural Differentiation in Plant Cell Cultures of Atropa belladonna and Hyoscyamus muticus. In: Secondary Metabolism in Plant Cell Culture, Morris, P., A.H. Serage, A. Stafford and M.W. Fowler (Eds.). Cambridge University Press, Cambridge, pp: 82.
- Gomez, K.A. and A.A. Gomez, 1984. Statistical Procedures for Agricultural Research. 2nd Edn., John Wiley and Sons Inc., Hoboken, New Jersey, ISBN: 978-0-471-87092-0, Pages: 704.
Direct Link - Gamborg, O.L., R.A. Miller and K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res., 50: 151-158.
CrossRefPubMedDirect Link