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Mutagenic Potentials of Dental Alloys Using Ames Salmonella/microsome Test

Elham Haji-Sayyari , Saeed Noukar , Mehran Mohseni , Mohammad Reza Fazeli , Hossein Jamalifar and Ebrahim Azizi

The potential mutagenicity of two Nickel-Chromium based alloys (Verabond II and Minalux) was determined using the Ames Salmonella/microsome test. According to ISO 6871-1 protocol, the alloys were mounted in an artificial saliva medium and incubated for 7 days at 37°C. Then aliquots were used to assess mutagenic effects of the released materials using Salmonella typhimurium strain TA 100 and the standard plate incorporation assay in the presence or absence of S9 fraction from rat liver. No mutagenic effects were detected for either alloys at 25, 50, 100 μL of test solutions. The S9 bioactivation also showed no mutagenic metabolite activities for these alloys. Therefore, it seems that both alloys have no mutagenic activity with or without bioactivation under the test conditions.

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  How to cite this article:

Elham Haji-Sayyari , Saeed Noukar , Mehran Mohseni , Mohammad Reza Fazeli , Hossein Jamalifar and Ebrahim Azizi , 2006. Mutagenic Potentials of Dental Alloys Using Ames Salmonella/microsome Test . Journal of Biological Sciences, 6: 106-108.

DOI: 10.3923/jbs.2006.106.108


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