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Journal of Biological Sciences

Year: 2006 | Volume: 6 | Issue: 6 | Page No.: 1035-1040
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Research Article

In vitro Propagation of Endangered Iris Species

Ahmad Al-Gabbiesh, Dhia S. Hassawi and Fatma U. Afifi

ABSTRACT

Calli from Iris petrana and I. atrofusca were produced from flower bases, leaf bases and anthers. The flower bases responded positively to the culture conditions and gave the best results. Callus induction medium was Murashige and Skoog (MS) supplemented with 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 μM Kinetin and 4.5 μM 1-naphthaleneacetic acid (NAA). Formation of green structures was noticed on calli of both species after transferring calli to callus culture medium (MS supplemented with 4.5 μM N6-benzyladenine (BA), 0.45 μM 2,4-D and 4.5 μM NAA). Embryo-like structures were appeared after transferring calli to embryogenesis induction medium (MS supplemented with 4.5 μM 2,4-D, 0.5 μM Kinetin, 4.5 μM NAA and 300 mg L-1 proline). Both green and albino plantlets were observed after calli were transferred to regeneration medium {MS supplemented with 4.5 μM BA, 0.45 μM 2,4-D and 0.49 μM indol-3-butyric acid (IBA)}.
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REFERENCES

  1. Al-Eisawi, D.M., 1986. Studies on the Flora of Jordan. Monocotyledon new to Jordan, with notes on saome interesting species. Kew Bull., 41: 349-357.
  2. Carlson, P.S., 1973. Methionine-sulfoximine resistant mutants of tobacco. Science, 180: 1366-1368.
  3. Gozu, Y., Y. Mineyuki, N. Masahiro and N. Ryujiro et al., 1993. In vitro propagation of Iris pallida. Plant Cell Rep., 13: 12-16.
  4. Hussey, G., 1975. Totipotency in tissue explants and callus of some members of the Liliaceae, Iridaceae and Amaryllidaceae. J. Exp. Bot., 26: 253-262.
    CrossRefDirect Link
  5. Hussey, G., 1976. In vitro release of auxiliary shoots from apical dominance in monocotyledonous plantlets. Ann. Bot., 40: 1323-1325.
  6. Hussey, G., 1976. Propagation of Dutch Iris by tissue culture. Sci. Hortic., 4: 163-165.
  7. Jehan, H., D. Courtois, C. Ehret, K. Lerch and V. Petiard, 1994. Plant regeneration of Iris pallida. Lam. and Iris germanica L. via somatic embryogenesis from leaves, apices and young flowers. Plant Cell Rep., 13: 671-675.
    CrossRef
  8. Laublin, G., S. Hargurdeep and S. Mario, 1991. In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises. Plant Cell Tissue Organ Cult., 27: 15-21.
  9. Murashige, T., 1974. Plant propagation through tissue cultures. Annu. Rev. Plant Physiol., 25: 135-166.
    CrossRefDirect Link
  10. Radojevic, L.J., O. Sokic and B. Tucic, 1987. Somatic embryogenesis in tissue culture of iris (I. Pumila L.). Acta. Hortic., 212: 719-723.
  11. Radojevic, L. and A. Subotic, 1992. Plant Regeneration of Iris setosa Pall. J. Plant Physiol., 139: 690-696.
  12. Reuther, G., 1977. Embryoide Differenzierungsmuster im kallus der Gattungen Iris und Asparagus. Ber. Deutsch. Bot. Ges., 90: 417-437.
  13. Shibli, R.A. and M.M. Ajlouni, 2000. Somatic embryogenesis in the endemic black Iris. Plant Cell Tissue Organ Cult., 61: 15-21.
    Direct Link
  14. Shimizu, K., H. Nagaike, T. Yabuya and T. Adachi, 1997. Plant regeneration from suspension culture of Iris germanica. Plant Cell Tissue Organ Cult., 50: 27-31.
  15. Vasil, I.K. and V. Vasil, 1972. Totipotency and Embryogenesis in plant cell and tissue culture. In Vitro, 8: 117-127.
    Direct Link
  16. Williams, C.A. and J.B. Harborne, 1985. Bioflavonoids, quinines and xanthones as rare chemical markers in the family Iridaceae. Z. Naturforsch, 40: 325-330.
  17. Jungnickel, F. and S. Zaid, 1992. Micropropagation of African Violets (Saintpaulia spp. and cvs.). In: Biotechnology in Agriculture and Forestry, Bajaj, Y.P.S. (Ed.). Springer-Verlag, Berlin, pp: 357-395.
  18. Al-Eisawi, D.M., A. El-Oqlah, S. Oran and J. Lahham, 2000. Jordan Country Study on Biological Diversity: Plant Biodiversity and Taxonomy. United Nations Environment Program, Jordan, pp: 72.
  19. Al-Khader, I.A., 1997. Systematic revision of the genus Iris (Iridaceae) in Jordan. M.Sc. Thesis, University of Jordan, pp: 2.
  20. Allan, E., 1991. Plant Cell Culture. In: Plant Cell and Tissue Culture, Stafford, A. and G. Warren (Eds.). Wiley Publishers, Chichester, UK., ISBN-13: 9780471932611, pp: 1-23.
  21. Caponetti, J.D., 1996. Nutrition of Callus Culture. In: Plant Tissue Culture, Concepts and Laboratory Exercises, Trigiano, R.N. and D.J. Gray (Eds.). CRC Press, Boca Raton, pp: 27-31.
  22. George, E.F., 1996. Plant Propagation by Tissue Culture. 2nd Edn., Exegetics Ltd., Edington, England, pp: 837-1207.
  23. Kareem, F.M. and S.A. Qor`an, 1988. Wild Flowers in Jordan. 1st Edn., Yarmouk University, Irbid.
  24. Laublin, G. and M. Cappadocia, 1990. Plant regeneration via somatic embryogenesis in several Iris species. Proceedings of 7th International Congress Plant Tissue and Cell Culture, (ICPTCC'1990), Amsterdam, pp: A3-A3.
  25. Mathew, B., 1985. The Iris. In: Flora of Iraq. Vol. 8, The Whitefriars Press Ltd, Baghdad, Iraq.
  26. Narayanaswamy, S., 1977. Regeneration of Plants from Tissue Cultures. In: Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, Reinert, J. and Y.P.S. Bajaj (Eds.). Springer Verlag, Berlin, Heidelberg, pp: 179-248.
  27. Nickel, L.G. and J.G. Torrey, 1969. Crop improvement through plant cell and tissue culture. Science, 166: 1068-1070.
    Direct Link
  28. Pierik, R.L.M., 1987. In vitro Culture of Higher Plants. 1st Edn., Martinus Nijhoff Publishers, Dordrecht, The Netherlands.
  29. Radojevic, L.J., O. Sokic and B. Tucic, 1985. Somatic embryogenesis in tissue culture of iris (Iris pumila L.). Proceedings of Symp. In vitro Problems Related to Mass Propagation of Horticultural Plants, Gembloux, pp: 25.
  30. Rasoul, T.N., 1984. Ornamental Bulbs. Al-Mousel University, Mosul.
  31. Reuther, G., 1975. Induktion der embryogenese in kalluskulturen. Planta Med. Suppl., 28: 42-58.
    CrossRefDirect Link
  32. Rodionenko, G.I., 1987. The Genus Iris L: Questions of Morphology, Biology, Evolution and Systematic. British Iris Society, London, pp: 222.
  33. Steel, R.G.D. and J.H. Torrie, 1960. Principles and Procedures of Statistics. McGraw-Hill, New York.
  34. Sutter, E.G., 1996. General Laboratory Requirements, Media and Sterilization Methods. In: Plant Tissue Culture, Concepts and Laboratory Exercises, Trigiano, R.N. and D.J. Gray (Eds.). CRC Press, Boca Raton, pp: 11-25.
  35. Tabata, M., 1977. Recent Advance in the Production of Medicinal Substances by Plant Cell Cultures. In: Plant Tissue Culture and its Biotechnological Applications, Barz, W., E. Reinhard and M.H. Zenk (Eds.). Springer-Verlag, Berlin, pp: 3-16.
  36. Torres, K., 1988. In vitro Propagation of African Violets. In: Tissue Culture Techniques for Horticultural Crops, Torres, K. (Eds.). Van Nostrand Reinhold Co., New York, pp: 80-85.
  37. Feinbrun-Dothan, N., 1986. Iridaceae. In: Flora Palaestina, Part 4, Zohari, M. and Feinbrun-Dothan (Eds.). Israel Academy of Sciences and Humanities, Jerusalem, pp: 112-127.

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