By taking into consideration symmetry of the position of Li vectors of enzymatic inhibited reactions to La vectors of activated enzymatic reactions in the three-dimensional K`mV`I coordinate system and symmetric antidirectivity of tendencies in the course of change of K`m and V` parameters of inhibited and activated enzymatic reactions similar by the type, a parametric classification of the types of enzymatic reactions was proposed, the equations for calculation of Ka constants of enzyme activation and Ki constants of nontrivial types of enzyme inhibition were obtained and some corrections introduced into practice of using the coordinates of intercepts and slopes for calculation of these constants. Examples of calculation of Ka and Ki constants are given.
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Vladimir I. Krupyanko, 2005. Additional Possibility of Data Analysis of Enzyme Inhibition and Activation.1: Equations for Calculation of the Ka and Ki Constants of Enzyme Activation and Nontrivial Types of Enzyme Inhibition. Journal of Biological Sciences, 5: 82-91.
- Krupyanko, V.I. and P.V. Krupyanko, 1999. Selection of substrate concentrations for determining the Michaelis constant and maximum rate of an enzymatic reaction. Applied Biochem. Microbiol., 35: 116-119.