In higher organisms most cell types exist in a quiescent state, G0. Upon activation, cells exit from G0 and enter the proliferative phases of the cell cycle. Rapamycin-FKBP12 is a potent inhibitor of proliferation of many cell types. In BC3H1 myogenic cells, rapamycin induces differentiation while inhibiting cellular proliferation. Since terminal differentiation requires withdrawal from the cell cycle, we have studied various cell-cycle regulators, the differentiation factor myogenin and the inhibitor of differentiation, which might be involved in this process. In the present study, we show that myogenic differentiation in the presence of rapamycin is coupled with the inhibition of phosphorylation of retinoblastoma protein (pRB) and sustenance of myogenin without affecting inhibitor of differentiation (Id) protein.
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Malathi Krishnamurthy, Michael Silane, Alejandro Berenstein and Thottala Jayaraman, 2005. Rapamycin Regulates Myogenesis by Inhibiting Myogenin Downregulation in BC3H1 Muscle Cells. Journal of Biological Sciences, 5: 61-65.
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