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Research Article

Influence of Ice Storage on the Gel Forming Ability, Myofibrillar Protein Solubility and Ca2+-ATPase Activity of Queen Fish (Chorinemus lysan)

M.I. Hossain , M. Kamal , M.N. Sakib , F.H. Shikha , M. Neazuddin and M.N. Islam
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An investigation was carried out on the quality changes in surimi prepared from ice stored Queen fish (Chorinemus lysan) in time interval during 20 days of storage in ice storage condition and the ratio of the fish to ice was maintained 1:1. Mince was prepared in both washed and unwashed condition. To evaluate the gel-forming characteristics, a portion of the mince was washed with water containing 0.1% NaCl. Both washed and unwashed mince were ground with 3% NaCl for 20 min at 4°C. The ground paste was stuffed into polyethylene tube and incubated at various temperatures (40, 50 and 60°C) for 2 h. The quality changes during ice storage of mince prepared from the ice stored fish muscle were evaluated with time interval by determining the gel-forming ability, myofibrillar Ca2+-ATPase activity, protein solubility and pH value of fish muscle. The resulting gels were subjected to the puncture test, teeth cutting test and folding test. Maximum breaking force was obtained from both washed and unwashed mince at the incubation temperature of 50°C. The gel strength of both unwashed and washed meat paste gradually declined with lapse of storage period and washed meat paste showed higher gel forming ability than unwashed meat paste throughout the study period. Myofibrillar Ca2+-ATPase activity, protein solubility and pH of the ice stored muscles decreased from 0.847 to 0.309 μmol pi min 1 mg 1, 87 to 12% and 6.69 to 5.89, respectively, during 20 days of ice storage.

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  How to cite this article:

M.I. Hossain , M. Kamal , M.N. Sakib , F.H. Shikha , M. Neazuddin and M.N. Islam , 2005. Influence of Ice Storage on the Gel Forming Ability, Myofibrillar Protein Solubility and Ca2+-ATPase Activity of Queen Fish (Chorinemus lysan). Journal of Biological Sciences, 5: 519-524.

DOI: 10.3923/jbs.2005.519.524



1:  Ishikawa, S., K. Nakamura and Y. Fujii, 1977. Fish jelly product (Kamaboko) and frozen minced meat (Frozen surimi) made of sardine. I. Freshness and handling of the fish material affecting its kamaboko forming ability. Bull. Tokai Reg. Fish. Res. Lab., 90: 59-66.

2:  Fukuda, Y., Z. Tarakita and K. Arai, 1984. Effect of chub mackerel on the freeze-denaturation of myofibrillar proteins. Nippon Suisan Gakkaishi, 50: 845-852.

3:  Roura, S.I., C. Monteccia, A.L. Goldemberg, R.E. Truco and M. Crupkin, 1990. Biochemical and physiochemical properties from pre- and post-spawned hake (Merluccius hubbsi) stored in ice. J. Food Sci., 55: 688-692.

4:  Kamal, M., S. Watabe and K. Hashimoto, 1991. Post-mortem changes of myofibrillar ATPase of sardine ordinary and dark muscle. Nippon Suisan Gakkaishi, 57: 1177-1184.

5:  MacDonald, G.A and T.C. Lanier, 1994. Actomyosin stabilization to freeze-thaw and denaturation by lactate salts. J. Food Sci., 59: 101-105.

6:  Ouali, A. and C. Valin, 1981. Effect of muscle lysosmal enzymes calcium activated neutral proteinases on myofibrillar ATPase activity: Relationship with aging changes. Meat Sci., 5: 233-245.

7:  Perry, S.V. and T.C. Grey, 1956. A Study on the effect of substrate concentration and certain relaxing factors on the magnesium activated myofibrillar adenosine triphosphate. Biochem. J., 64: 184-192.

8:  Poon, K.H., P.Y. Lim, M.C. Ng and P.C. Ng, 1981. The suitability of leached meat of small demersal fish for making fish jelly products. Singapore J. Primary Ind., 9: 28-37.

9:  Shimizu, Y., R. Machida and S. Takenami, 1981. Species variation in the gel forming ability characteristics of fish meat paste. Nippon Suisan Gakkaishi, 47: 95-98.

10:  Fisk, C.H. and Y. Subbarow, 1925. The colometric determination of phosphorus. J. Biol. Chem., 66: 375-400.

11:  Nowsad, A.K.M., S.C. Chanda, S. Kanoh and E. Niwa, 2000. Gel forming ability and other properties of eleven underutilized tropical marine fish species. J. Aquat. Prod. Technol., 9: 71-89.

12:  Suwansakornkul, P., Y. Itoh, S. Hara and A. Obatake, 1993. The gel forming characteristics of fish meat paste. Nippon Suisan Gakkaishi, 59: 1029-1037.

13:  Hossain, M.I., M.M. Kamal, F.H. Shikha and A.M.S. Hoque, 2004. Effect of washing and salt concentration on the gel forming ability of two tropical fish species. Int. J. Agric. Biol., 6: 762-766.
Direct Link  |  

14:  Kurokawa, T., 1979. Kamaboko-forming ability of frozen and ice stored lizard fish. Bull. Jap. Soc. Sci. Fish., 45: 1551-1551.

15:  Kramer, D.E. and M.D. Peters, 1981. Effect of pH and prefreezing treatment on the texture of yellowtail rockfish (Sebastes flavidus) as measured by the Ottawa texture measuring system. Int. J. Food Sci. Technol., 16: 493-504.
CrossRef  |  Direct Link  |  

16:  Shimizu, Y., W. Simidu and T. Ikeuchi, 1954. Studies on jelly strength of kamaboko-III. Influence of pH on jelly strength. Bull. Jap. Soc. Sci. Fish., 20: 209-212.

17:  Gill, T.A., R.A. Keith and B. Smith, 1979. Textural deterioration of red hake and haddock muscle in frozen storage as related to chemical parameters and changes in the myofibrillar proteins. J. Food Sci., 44: 661-661.

18:  Lim, H.K. and N.F. Haard, 1984. Protein insolubilization in frozen Greenland halibut (Reinhardtius hippoglossoides). J. Food. Biochem., 8: 163-187.

19:  Tejada, M., C.P. Torrejon, M.L. Del-Mazo, M.T. Solas, M.L. Garcia and C. Barba, 1996. Protein extracts and aggregates forming in minced cod (Gadus morhua) during frozen storage. J. Agric. Food Chem., 44: 3308-3314.

20:  Anderson, M.L. and E.M. Ravesi, 1970. On the nature of the association of protein in frozen stored cod muscle. J. Food Sci., 35: 551-558.
Direct Link  |  

21:  Benjakul, S. and F. Bauer, 2000. Physicochemical and enzymatic changes of cod muscle proteins subjected to different freeze-thaw cycles. J. Food Sci. Agric., 80: 1143-1150.
CrossRef  |  Direct Link  |  

22:  Portzehl, H., P. Zaoralek and J. Gaudian, 1969. The activation by Ca2+ of the ATPase of extracted muscle fiber with variation of ionic strength pH and concentration of Mg2+ ATP. Biochim. Biophys. Acta, 189: 440-448.

23:  Gornall, A.G., C.J. Bardawill and M.M. David, 1949. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem., 177: 751-766.
PubMed  |  Direct Link  |  

24:  Haard, N.F. and J.E. Warren, 1985. Influence of halding fillets from undersize atlantic cod (Gadus morhua) at 0 or 3°C on the yield and quality of surimi. Proceedings of the International Symposium on Engineered Seafood Including Surimi, Nov. 19-21, National Fisheries Institute, Washington, DC., pp: 92-92

25:  Noguchi, S., 1982. Science of Frozen Surimi I, II, III. Practical Technical Handbook for Kneaded Seafoods. Nippon, Shokuhin Kelzai-sha,Tokyo, pp: 40

26:  Joseph, J. and P.A. Perigreen, 1983. Studies on frozen storage of minced fish from thread fin bream. Fish Technol., pp: 20:13.

27:  Jiang, S.T., D.T.Y. Chu, C.C. Lan and T.C. Lee, 1984. New approach to improve the quality of minced fish products using freeze-thawed cuttle fish. Proceeding of Chinese-American Academic and Professional Convention. S.3.3.1-S3.3.5, Jun. 29-Jul. 2, Los Angeles, CA

28:  Bendall, J.R., 1960. Post-mortem Changes in Muscle. In: Structure and Function of Muscle, Bourne, G.H.M. (Ed.). Vol. 3, Academic Press, New York, pp: 227

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