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Journal of Biological Sciences

Year: 2005 | Volume: 5 | Issue: 2 | Page No.: 239-242
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Research Article

Evaluation of the Antioxidant Activity of the Secondary Metabolites from Piliostigma reticulatum (DC.) Hochst

M.A. Aderogba, E.K. Okoh and T.O. Idowu

ABSTRACT

The ethyl acetate extract of Piliostigma reticulatum leaves was investigated for its antioxidant constituents. Isolated secondary metabolites of the leaves extract include: 2-O-(p-hydroxyphenyl)-5-hydroxy-6-C-methyl-7-methoxychromone (piliostigmin) 1, 6-C- methylkaempferol- 3-methyl ether 2, quercetin 3, quercetrin (quercetin-3-O-rhamnose) 4, and Isoquercetrin (quercetin-3-O-glucoside) 5. These compounds were evaluated for their antioxidant potentials. A rapid test for antioxidants using DPPH (1,1-dipheny-2-picryhydrazyl) TLC screening, demonstrated considerable radical scavenging activity for all the compounds. The DPPH spectrophotometric method was employed for quantitative determination of radical scavenging activity. The activity was expressed as EC50 value, which ranges between 4.68±0.03 and 50.76±1.71 for the flavonoids constituent.
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REFERENCES

  1. Burits, M. and F. Bucar, 2000. Antioxidant activity of Nigella sativa essential oil. Phytother. Res., 14: 323-328.
    CrossRefPubMedDirect Link
  2. Tutour, B.L., 1990. Antioxidative activities of algal extracts, synergistic effect with vitamin E. Phytochemistry, 29: 3759-3765.
    CrossRef
  3. Ito, N., S. Fukushima, A. Haqlwara, M. Shibata and T. Ogiso, 1983. Carcinogenicity of butylated hydroxyanisole in F344 rats. J. Natl. Cancer Inst., 70: 343-352.
    CrossRefDirect Link
  4. Aderogba, M.A., E.K. Okoh, A.O. Ogundaini and B.M. Abegaz, 2003. 6-C-Methylquercetin-3,3N,4N-trimethyl ether from the leave extract of Piliostgma reticulatum (DC.) Hochst. Nig. J. Natl. Prod. Med., 7: 37-38.
  5. Ibewuike, J.C., F.O. Ogungbamila, A.O. Ogundaini, I.N. Okeke and L. Bohlin, 1997. Antiinflammatory and antibacterial activities of C-methylflavonols from Piliostigma thonningii. Phytother. Res., 11: 281-284.
    CrossRefDirect Link
  6. Havstein, B., 1983. Flavonoids a class of natural products of high pharmacological potency. Biochem. Pharmacol., 32: 1141-1148.
    PubMed
  7. Alcaraz, M.J. and M.J. Jimenez, 1988. Flavonoids as anti-inflammatory agents. Fitoterapia, 58: 25-38.
  8. Mensor, L.L., F.S. Menezes, G.G. Leitao, A.S. Reis, T.C. dos Santos, C.S. Coube and S.G. Leitao, 2001. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother. Res., 15: 127-130.
    CrossRefPubMedDirect Link
  9. Thabrew, M.I., R.D. Hughes and I.G. McFarlane, 1998. Antioxidant activity of Osbeckia aspera. Phytother. Res., 12: 288-290.
  10. De Beck, P.O., G. Cartier, B. David, M.G. Dijoux-Franca and A.M. Mariotte, 2003. Antioxidant flavonoids and phenolic acids from leaves of Leea guineense G. Don (Leeaceae). Phytother. Res., 17: 345-347.
    CrossRefPubMed
  11. Trouillas, P., C. Fagnere, R. Lazzaroni, C.A. Caliste, A. Marfak and J.L. Duroux, 2004. A theoretical study of the conformational behavior and electronic structure of taxifolin correlated with the free radical scavenging activity. Food Chem., 88: 571-582.
    Direct Link
  12. Van Acker, S.A.B.E., D.J. van den Berg, M.N.J.L. Tromp, D.H. Grifioen, W.P. van Bennekom, W.J.F. van der Vijgh and A. Bast, 1996. Structural aspects of antioxidant activity of flavonoids. Free Radic. Biol. Med., 20: 331-342.
    CrossRefPubMedDirect Link
  13. Assi, L.A. and S. Guinko, 1991. Plant Used in Traditional Medicine in West Africa. Swiss Centre for Scientific Research, Adiopodoume, Abidjan, pp: 17-18.
  14. Burkill, H.M., 1995. The Useful Plants of West Africa Tropical Africa. 2nd Edn., Vol. 3, Royal Kew Botanical Gardens, Kew, London, pp: 144-150.
  15. Virgili, F., C. Scaccini, L. Packer and G. Rimbach, 2001. Cardiovascular Disease and Nutritional Phenolics. In: Antioxidants in Food, Pokorny, J., N. Yanishlieva and G. Rimbach (Eds.). Woodhead Publishing Ltd., Cambridge, ISBN: 1-85573-463-X, pp: 87-99.

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