The shoot multiplication rate is most significant in terms of shoot production. Cytokinins are usually added to tissue culture media to stimulate axillary or adventitious shoot development. The type and concentration of cytokinin used have profound effects on shoot multiplication. Rates of shoot induction of Moringa nodes in presence of Benzylaminopurine (BAP) were observed. Hundred percent explants induced shoots in presence of 1.0 and 1.5 mg L-1 BAP concentrations. But in terms of highest shoot production, the 1.0 mg L-1 BAP conc. was best. Highest shoot multiplication rate was found in the 3rd subculture on it. The regenerated shoots were rooted on MS (Murashiege and Skoog) medium alone and fortified with different concentrations of NAA, IBA and IAA. The maximum percentage of rooting was obtained on MS medium alone. The rooted plantlets were hardened and successfully established in the soil.
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Shahina Islam, Miskat Ara Akther Jahan and Rahima Khatun, 2005. In vitro Regeneration and Multiplication of Year-round Fruit Bearing Moringa oleifera L. Journal of Biological Sciences, 5: 145-148.