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Research Article
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Effective Microorganisms for Enhancing Pleurotus ostreatus (Fr.)
Kummer Production |
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Sopit Vetayasuporn
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ABSTRACT
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In the present study 0, 0.5, 1.0, 2.0, 4.0 and 8 mL of Effective
Microorganisms (EM) concentrations were diluted with 1000 mL of tap-water and at
each concentration was used to mix with Pleurotus ostreatus solid
culture. Growth of P. ostreatus mycelia were scrutinized during 5 weeks
of incubation period. Rapid growth of P. ostreatus mycelia were revealed
in the EM mixed P. ostreatus solid culture. Twenty percent contamination
were found in the EM unmixed P. ostreatus growth substrates while 0-5%
were detected in the EM mixed P. ostreatus solid culture. Averages of
total mushroom weight and number of oyster mushrooms (OMs.) were high when
watering P. ostreatus with the tap-water instead of EM solution.
0.43-0.63 kg of the total mushroom weight and 69-110 of OMs. were detected per
block. Mean total mushroom weight and number of OMs. per block was 2.0 to 4.0
times higher in the EM mixed than unmixed P. ostreatus growth substrates.
However, it was found that irrigation of OM cultures using ordinary tap water
and irrigation using EM dissolved in tap water, gave almost insignificant
differences in average weight, width and height of OMs. at a confidence level of
95%, where the average weight, width and height of each OM was found to be
approximately 6.26 g, 5.96 and 7.89 cm, respectively.
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REFERENCES |
1: Bobek, P., E. Ginter, M. Jurcovicova and L. Kuniak, 1991. Cholesterol-lowering effect of the mushroom Pleurotus ostreatus in hereditary hypercholesterolemic rats. Ann. Nutr. Metab., 35: 191-195. PubMed |
2: Bunyard, B.A., S. Chaichuchote, M.S. Nicholson and D.J. Royse, 1996. Ribosomal DNA analysis for resolution of genotypic classes of Pleurotus. Mycol. Res., 2: 143-150.
3: Bano, Z. and S. Rajarathnam, 1988. Pleurotus mushrooms. Part 2. Chemical composition, nutritional value, post-harvest physiology, preservation and role as human food. discipline of fruits, vegetables and plantation crops, central food technological research institute, Mysore, India. Crit. Rev. Food Sci. Nutr., 27: 87-158.
4: Higa, T., 2001. The Technology of Effective Microorganisms-Concept and Philosophy. University of the Ryukyus, Okinawa, Japan
5: Albersheim, P., 1976. The Primary Cell Wall. In: Plant Biochemistry, Bonner, J. and J.E. Varner (Ed.). Academic Press, New York, pp: 225-274
6: Clarke, A.J., 1997. Biodegradation of Cellulose: Enzymology and Biotechnology. Technomic Pub. Co., Lancaster, PA
7: Keller, B., 1993. Structural cell wall proteins. Plant Physiol., 101: 1127-1130. Direct Link |
8: Nielsen, J., 1996. Modelling the growth of filamentous fungi. Adv. Biochem. Eng. Biotechnol., 46: 187-223.
9: Moo-Young, M., A.R. Moreina and R.P. Tengerdy, 1975. Principles of Solid-Substrate Fermentation. In: The Filamentous Fungi, Smith, J.E. and D.R. Berry (Eds.). Vol. 1, Edward Arnold London, London
10: Trinci, A.P.J., 1984. Regulation of Hyphal Branching and Hyphal Orientation. In: The Ecology and Physiology of the Fungal Mycelium, Jennings, D.H. and A.D.M. Rayner (Eds.). Cambridge University Press, Cambridge
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