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Journal of Biological Sciences

Year: 2004 | Volume: 4 | Issue: 4 | Page No.: 547-552
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Research Article

Effect of Microcystin from Jordan on Ion Regulation and Antioxidant System in the Hepatocytes of Mice

S. Al-Jassabi

ABSTRACT

The objective of this study was to evaluate mechanisms of microcystin (from King Talal Reservoir, Jordan) toxicity on the hepatocytes of mice. Five to seven weeks old male albino Balb/C mice (about 30 g wt.) were injected i.p. with normal saline (as control) or Microcystis aeruginosa aqueous extract (30 mg L-1) at 24 h intervals for 48 h. After the exposure period of toxin-treated and control mice, Na+, K+-ATPase and glutathione-S-transferase (GST) activities were measured in hepatocytes extracts. Total oxyradical scavenging capacity (TOSC) and lipid peroxides (LPO) content were also determined. Na+, K+-ATPase and GST in the hepatocytes of mice was significantly lower in toxin treated mice than in control ones. Mice exposed to microcystin also showed a significant increase in the TOSC value against peroxyl radicals. Lipid peroxides level did not change in the hepatocytes of mice after exposure to the toxin. The increased levels of TOSC suggest on the occurrence of mice response against oxidative stress induced by toxin injection, which prevents lipid peroxidation.
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REFERENCES

  1. Carmichael, W.W., 1994. The toxins of cyanobacteria. Sci. Am., 270: 78-86.
    PubMed
  2. Fischer, W.J. and E. Dietrich, 1999. Pathological and biochemical characterization of microcystin-induced hepatopancreas and kidney damage in Carp Cyprinus carpio. Toxicol. Applied Pharmacol., 164: 73-81.
  3. Mazur, H. and M. Plinski, 2001. Stability of cyanotoxins, microcystin-LR, microcystin-RR and nodularin in seawater. Oceanologia, 43: 329-339.
  4. Robinson, N., C. Miura, R. Matson, E. Dinternan and J. Pace, 1989. Characterization of chemically treated microcystin-LR and its distribution on mice. Toxicon, 27: 1035-1042.
  5. Ding, W.X. and C.N. Ong, 2003. Role of oxidative stress and mitochondrial changes in cyanobacteria-induced apoptosis and hepatotoxicity. FEMS Microbiol. Lett., 220: 1-7.
    CrossRefDirect Link
  6. Runnegar, M., N. Berndt, S.M. Kong, E.Y.C. Lee and L. Zhang, 1995. In vivo and in vitro binding of microcystin to protein phosphatase 1 and 2A. Biochem. Biophys. Res. Commun., 216: 162-169.
    CrossRef
  7. Pinho, G.L.L., C. Moura, J.S. Yunes and J.M. Monserrat, 2003. Toxic effects of microcystins in the hepatopancreas of the estuarine crab Chasmagnathus granulatus (Decapoda, Grabsidae). Comp. Biochem. Physiol., Part C, 135: 459-468.
  8. Park, H., M. Namikoshi, S.M. Brittain, W.W. Carmichael and T. Murphy, 2001. Microcystin-LR, a new microcystin isolated from waterbloom in a Canadian prairic lake. Toxicon, 39: 855-862.
  9. Bury, N.R., G.A. Codd, S.E.W. Bonga and G. Flik, 1998. Fatty acids from the cyanobacterium Microcystis aeruginosa with potent inhibitory effects on fish gill Na, K-ATPase activity. J. Exp. Biol., 201: 81-89.
  10. Ding, W.X., H.M. Shen and C.N. Ong, 2000. Microcystic cyanobacteria extract induces cytoskeletal disruption and intracellular glutathione alteration in hepatocytes. Environ. Health Perspect., 108: 605-609.
  11. Rauchova, H., Z. Drahota and J. Koudelova, 1999. The role of membrane fluidity changes and thiobarbituric acid-reactive substances production in the inhibition of cerebral cortex Na+,K+-ATPase activity. Physiol. Res., 48: 73-78.
    PubMedDirect Link
  12. Castilho, P.C., I.A. Martins and A. Bianchini, 2001. Gill Na+, K+-ATPase and osmoregulation in the estuarine crab, Chasmagnathus granulata Dana, 1851 (Decapoda, Grabsidae). J. Exp. Mar. Biol., 256: 215-227.
  13. Vinagre, T.M., J.C. Alciati, F. Regoli, J.S. Younes and J.M. Monserrat, 2003. Effect of microcystin on ion regulation system in gills of the estuarine crab Chasmagnathus granulatus (Decapoda, Grapsidae). Comp. Biochem. Physiol. Part C, 135: 67-75.
  14. Bertorello, A.M. and A.I. Katz, 1995. Regulation of Na-K pump activity: Pathways between receptors and effectors. NIPS., 10: 253-259.
  15. Bury, N.R., E.R. Eddy and G.A. Codd, 1996. The effect of cyanobacterium Microcystis aeruginosa, the cyanobacterial hepatotoxin microcystin-LR and ammonia growth rate and ionic regulation of brown trout. J. Fish Biol., 46: 1042-1054.
  16. Jamme, I., E. Petit, D. Divoux , A. Gerbi, J.M. Maixent and A. Nouvelot, 1995. Modulation of mouse cerebral Na, K-ATPase activity by oxygen free radicals. Neuro. Rep., 7: 333-337.
  17. Gaete,V., E. Canclo, N. Lagos and E. Zambrano, 1994. Inhibitory effects of Microcystis aeruginosa toxin on ion pumps of the gill of freshwater fish. Toxicon, 32: 121-127.
  18. Matthienscn, A., K.A. Beattie, J.S. Yunes, K. Kaya and G.A. Codd, 2000. Microcystin-LR, from the cyanobacterium Microcystis RST 9501 and from Microcystis bloom in the patos Lagoon estuary, Brazil. Photochemistry, 55: 383-387.
  19. Sakar, M.L., B.A. Neilan and D.J. Griffiths, 2000. Two morphological forms of Clindrospermopsis raciborskii isolated from Solomon Dam, Palm island. Queensland J. Phycol., 35: 599-606.
  20. Lawton, A., C. Edwards and A. Codd, 1994. Extraction and high performance liquid chromatography method for the determination of microcystins in raw and treated waters. Analyst, 119: 1525-1530.
  21. Habig, W.I. and W.B. Jakoby, 1981. Assays for differentiation of glutathion-S-transferase. Meth. Enzymol., 77: 398-405.
  22. Regoli, F. and G.W. Winston, 1999. Quantification of total oxidant scavenging capacity of antioxidants for peroxynitrite, peroxyl radicals and hydroxyl radicals. Toxicol. Applied Pharmacol., 156: 96-105.
  23. Winston, G.W., F. Regoli, A.J. Dugas, J. Fong and K.A. Blanchard, 1998. A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids. Free Rad. Biol. Med., 24: 480-493.
  24. Hermes-Lima, M., W.G. Willmore and K.B. Storey, 1995. Quantification of lipid peroxidation in tissue extracts based on Fe (III) xylenol orange complex formation. Free Rad. Biol. Med., 19: 271-280.
  25. Morhashi, M., K. Tsuchiya, T. Mita and M. Kawamura, 1991. Identification of (Na, K) ATPase inhibitor in brine shrimp, Artemia salina, as long-chain fatty acids. J. Comp. Physiol. Part B, 161: 69-72.
  26. Takenake, S., 2001. Covalent glutathione to cyanobacterial hepatotoxin microcystin LR by F344 rat cytosolic and microsomal glutathion-S-transferases. Environ. Toxicol. Pharmacol., 9: 135-139.
  27. Vitale, A.M., J.M. Monserrat, P.C. Castilho and E.M. Rodriguez, 1999. Inhibitory effects of cadmium on carbonic anhydrase activity and ionic regulation of estuarine crab Chasmagnathus granulataM (Decapoda, Grapsidae). Comp. Biochem. Physiol. Part C, 122: 121-129.
  28. Ding, W.X., H.M. Shen, H.G. Zhu and C.N. Ong, 1998. Studies on oxidative damage induced by cyanobacteria extract in primary cultured rat hepatocytes. Environ. Res., 78: 12-16.
  29. Rohn, T.T., T.R. Hinds and F.F. Vincnzi, 1993. Ion transport ATPases as targets for free radical damage. Protection by an aminosteroid of the Ca2 pump ATPase and Na/K pump ATPase of human red blood cell membranes. Biochem. Pharmacol., 46: 525-534.
  30. Vinagre, T.M., J.C. Alciati, J.S. Yunes, J. Richards, A. Bianchini and J.M. Monserrat, 2002. Effects of extracts from the cyanobacterium Microcystis aeruginosa on ion-regulation and gill Na, K-ATPase and K dependent phosphatase activities of the estuarine crab Chasmagnathus granulata (Decapoda, Grapsidea). Physiol. Biochem. Zool., 73: 181-187.

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