Submit Manuscript

Journal of Biological Sciences

Year: 2004 | Volume: 4 | Issue: 4 | Page No.: 532-536
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Research Article

Mutagenesis of Bacteria by Fibrous or Clay Minerals

Naoto Yoshida, Tadahumi Naka and Kazuyoshi Ohta

ABSTRACT

In the present cells of Escherichia coli, Agrobacterium radiobacter, or Thiobacillus intermedius were exposed to chrysotile asbestos, kaolinite, or bentonite by culture in medium containing these minerals. Chromosomal DNA was extracted from exposed cells, then amplified by the RAPD method and band patterns were compared. DNA of bacterial cells exposed to these minerals, was amplified, in whereas that in control cells was not, or that amplified in control cells disappeared. Not only asbestos, but also kaolinite and bentonite, which are principal soil components, induce bacterial mutation. Growth was inhibited by 66%, when A. radiobacter was cultured in LB medium containing 20 mg mL-1 of asbestos for 24 h. Growth inhibition by asbestos was reduced by adding 420 U mL-1 of catalase or 500 U mL-1 of superoxide dismutase to the culture medium.
PDF References Citation

Search

REFERENCES

  1. Churg, A., K. Zay and K. Li, 1997. Mechanisms of mineral dust-induced emphysema. Environ. Health Perspect., 105: 1215-1218.
  2. Furst, A. and M. Fan, 1993. Carcinogenic action of nickel and cadmium powders in the same rat. Biol. Trace Element Res., 36: 243-249.
  3. Sky-Peck, H.H., 1896. Trace metals and neoplasia. Clin. Physiol. Biochem., 4: 99-111.
  4. Mossman, B.T. and J.B. Gee, 1989. Asbestos-related diseases. N. Engl. J. Med., 320: 1721-1730.
  5. Mossman, B.T., J. Bignon, M. Corn, A. Seaton and J.B. Gee, 1990. Asbestos: Scientific developments and implications for public policy. Science, 247: 294-301.
    CrossRef
  6. Yoshida, N., Y. Murooka and K. Ogawa, 1998. Heavy metal particle resistance in Thiobacillus intermedius 13-1 isolated from corroded concrete. J. Ferment. Bioeng., 85: 630-633.
    CrossRef
  7. Yoshida, N., T. Yoshida and K. Ogawa, 1999. Toxicity of cadmium particle dust in bacterial cells. Biosci. Biotechnol. Biochem., 63: 1463-1466.
    CrossRef
  8. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535.
    CrossRefPubMedDirect Link
  9. Yoshida, N., T. Morinaga and Y. Murooka, 1993. Characterization and identification of bacterial strains isolated from corroded concrete in the accumulation stratum and their resistance levels to heavy metals. J. Ferment. Bioeng., 76: 400-402.
    CrossRef
  10. Weitzman, S.A. and P. Graceffa, 1984. Asbestos catalyzes hydroxyl and superoxide radical generation from hydrogen peroxide. Arch. Biochem. Biophys., 228: 373-376.
    CrossRef
  11. Heintz, N.H., Y.M. Janssen and B.T. Mossman, 1993. Persistent induction of c-fos and c-jun expression by asbestos. Proc. Nat. Acad. Sci. USA., 90: 3299-3303.
    CrossRefDirect Link
  12. Ashby, J.M. and I.F. Purchase, 1977. The selection of appropriate chemical class controls for use with short-term tests for potential carcinogenicity. Ann. Occup. Hyg., 20: 297-301.
    CrossRefDirect Link
  13. Weitzman, S.A. and A.B. Weitberg, 1985. Asbestos-catalysed lipid peroxidation and its inhibition by desferroxamine. Biochem. J., 225: 259-262.
    CrossRefDirect Link
  14. Turver, C.J. and R.C. Brown, 1987. The role of catalytic iron in asbestos induced lipid peroxidation and DNA-strand breakage in C3H10T1/2 cells. Br. J. Cancer, 56: 133-136.
    Direct Link
  15. Faux, S.P., P.J. Howden and L.S. Levy, 1994. Iron-dependent formation of 8-hydroxydeoxyguanosine in isolated DNA and mutagenicity in Salmonella typhimurium TA102 induced by crocidolite. Carcinogenesis, 15: 1749-1751.
    CrossRef
  16. Yoshida, N., T. Ikeda, T. Yoshida, T. Sengoku and K. Ogawa, 2001. Chrysotile asbestos fibers mediate transformation of Escherichia coli by exogenous plasmid DNA. FEMS Microbiol. Lett., 195: 133-137.
    CrossRef

Search

Leave a Comment

Your email address will not be published. Required fields are marked *