Submit Manuscript

Journal of Biological Sciences

Year: 2004 | Volume: 4 | Issue: 4 | Page No.: 521-526
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

ABSTRACT

The experiment was conducted in the Tissue Culture Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh, Bangladesh for micropropagation of tomato to evaluate the effect of variety and plant growth regulators in MS medium on shoot induction from virus infected calli of tomato plants. Three tomato varieties namely Bahar, Binatomato-2 and Binatomato-3 were used as plant materials in the present study. Callus derived shoots were induced on MS medium supplemented with different concentrations and combinations of plant growth regulators (PGRs). The combination of 0.2 mg L-1 IAA+4.0 mg L-1 BAP in MS medium was the best for inducing shoots which turned green to dark green after 15 days of culture. Callus derived shoots were fully virus infected which was confirmed by ELISA test. Meristem of plantlet can be used for the production of virus free tomato plant by meristem culture.
PDF References Citation

Search

REFERENCES

  1. Ahmad, K.U., 1976. Flower, Fruit and Vegetable. 3rd Edn., Alhaz Kamisuddin Ahmad, Dhaka, Bangladesh, pp: 470.
  2. Bose, T.K. and M.G. Som, 1986. Vegetable Crops in India. 1st Edn., Naya Prakash, Calcutta, pp: 262-264.
  3. BBS., 2000. Monthly statistical bulletin. Bangladesh Bureau of Statistics, Ministry of Planning, Government of the People's Republic of Bangladesh, pp: 55.
  4. FAO, 1999. FAO Quarterly Bulletin of Statistics. Food and Agriculture Organization, Rome, Italy, pp: 79-80.
  5. Villareal, E.R., 1980. Tomato in the Tropics. West View Press Inc., Baulder, Galordo, pp: 77.
  6. Sastry, K.S.M. and S.J. Singh, 1979. Control of the spread of tomato leaf virus by controlling the white fly population. Indian J. Hortic., 31: 178-182.
  7. Costa, G.M., F.T.S. Nogueira, W.C. Otoni and S.H. Brommonschenkel, 2000. In vitro regeneration of processing tomato (Lycopersicon esculentum Mill.) IPA-5 and IPA-6. Ciencia-e-Agrotecnologia, 24: 671-678.
  8. Gomez, K.A. and A.A. Gomez, 1984. Statistical Procedures for Agricultural Research. 2nd Edn., John Wiley and Sons Inc., Hoboken, New Jersey, ISBN: 978-0-471-87092-0, Pages: 704.
    Direct Link
  9. Chande, G. and S.K. Katiyar, 2000. Organogenesis and somatic embryogenesis in tomato (Lycopersicon esculentum Mill.). Advan. Plant Sci., 13: 11-17.
  10. Venkatachalam, P., N. Geetha, P. Priya, G. Rajaseger and N. Jayabalan, 2000. High frequency plantlet regeneration from hypocotyl explants of tomato (Lycopersicon esculentum Mill.) via organogenesis. Plant Cell Biotech. Mol. Biol., 1: 95-100.
    Direct Link
  11. Chen, H., J. Zhang, J. Zhong and J. Yu, 2000. Plant regeneration from hypocotyl explants cultured in vitro and histological observations in Lycoperscon esculentum Mill. Xibei Zhiwu Xuebao, 20: 759-765.
  12. Moghaieb, R.E.A., H. Saneoka and K. Fujita, 1999. Plant regeneration from hypocotyl and cotyledon explant of tomato (Lycopersicon esculentum Mill.). Soil Sci. Plant Nutr., 45: 639-646.
  13. Watterson, J.C., 1986. Diseases of the Tomato Crops. Chapman and Hill Ltd., New York, pp: 461-462.

Search

Related Articles

Leave a Comment

Your email address will not be published. Required fields are marked *