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Research Article
 

Genetic Diversity in Brassica Species Using SDS-PAGE Analysis



Rahman Md. Mukhlesur and Yutaka Hirata
 
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ABSTRACT

Eighty five different cultivars of Brassica rapa, B.juncea, B.napus, B. carinata, B. oleracea and hexaploid Brassica, collected from Bangladesh, Japan, China and Denmark, were analyzed for seed and leaf protein variations by SDS-PAGE to identify the polymorphic genetic markers for evaluation of genetic resources. Ten polymorphic markers were identified from seed protein and no identifiable polymorphic band was found from leaf protein. However, polymorphic markers clearly distinguished these Brassica species. Brassica rapa var. `yellow sarson` of Bangladesh origin showed uniquely identifiable four polymorphic bands for seed protein in contrast to the other B.rapa of brown-seeded type. The Bangladeshi and Japanese cultivars of B. rapa differed among protein quantity. Analytical results of SDS-PAGE for seed protein showed that hexaploid Brassica has the highest indices, such as % of polymorphic band, the degree of phenotypic diversity (Ho), diversity value for genetic marker (HEP) and the sum of the effective number of alleles (SENA). The genetic diversity values of hexaploid Brassica were followed by amphidiploid (B. napus, B. juncea, B. carinata) and diploid (B. oleracea, B. rapa) species, respectively.

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  How to cite this article:

Rahman Md. Mukhlesur and Yutaka Hirata , 2004. Genetic Diversity in Brassica Species Using SDS-PAGE Analysis. Journal of Biological Sciences, 4: 234-238.

DOI: 10.3923/jbs.2004.234.238

URL: https://scialert.net/abstract/?doi=jbs.2004.234.238

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