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Research Article

Genetic Diversity in Brassica Species Using SDS-PAGE Analysis

Rahman Md. Mukhlesur and Yutaka Hirata
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Eighty five different cultivars of Brassica rapa, B.juncea, B.napus, B. carinata, B. oleracea and hexaploid Brassica, collected from Bangladesh, Japan, China and Denmark, were analyzed for seed and leaf protein variations by SDS-PAGE to identify the polymorphic genetic markers for evaluation of genetic resources. Ten polymorphic markers were identified from seed protein and no identifiable polymorphic band was found from leaf protein. However, polymorphic markers clearly distinguished these Brassica species. Brassica rapa var. `yellow sarson` of Bangladesh origin showed uniquely identifiable four polymorphic bands for seed protein in contrast to the other B.rapa of brown-seeded type. The Bangladeshi and Japanese cultivars of B. rapa differed among protein quantity. Analytical results of SDS-PAGE for seed protein showed that hexaploid Brassica has the highest indices, such as % of polymorphic band, the degree of phenotypic diversity (Ho), diversity value for genetic marker (HEP) and the sum of the effective number of alleles (SENA). The genetic diversity values of hexaploid Brassica were followed by amphidiploid (B. napus, B. juncea, B. carinata) and diploid (B. oleracea, B. rapa) species, respectively.

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  How to cite this article:

Rahman Md. Mukhlesur and Yutaka Hirata , 2004. Genetic Diversity in Brassica Species Using SDS-PAGE Analysis. Journal of Biological Sciences, 4: 234-238.

DOI: 10.3923/jbs.2004.234.238


Bowman, K.D., K. Hutcheson, E.P. Odum and L.R. Shenton, 1971. Comments on the distribution of indices of diversity. Stat. Ecol. 3: 315-359.

Cong, T.V. and Y. Hirata, 2002. Seed storage protein diversity of three rice species in the Mecong Delta. Biosphere Conserv., 4: 59-67.

Das, S., R. Jyothi, B. Sabhyta, P.S. Srivastava and L. Malathi, 2000. Assessment of genetic variation within Brassica campestris cultivars using amplified fragment length polymorphism and random amplification of polymorphic DAN markers. Plant Molecular Biology Division, Tata Energy Research Institute, Lodhi Road, New Delhi, India.

Gepts, P. and F.A. Bliss, 1988. Dissemination pathway of common bean deduced from phaseolin electrophoretic variability. II. Eur. Afr., Econ. Bot., 42: 86-104.

Isemura, T., N. Shiyo, M. Shigeyuki, Y. Michihiro, N. Hiroo, I. Masayoshi and K. Osamu, 2001. Genetic variation and geographical distribution of Azuki bean (Vigna angularis) landraces based on the electrophoregram of seed storage proteins. Breed. Sci., 51: 225-230.

King, L.M. and B.A. Schaal, 1989. Ribosomal DNA variation and distribution in Rudbeckia missouriensis. Evolution, 43: 1117-1119.

Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski, 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol. Breed., 2: 225-238.
CrossRef  |  Direct Link  |  

Russell, J.R., J.D. Fuller, M. Macaulay, B.G. Hatz, A. Jahoor, W. Powell and R. Waugh, 1997. Direct comparison of level of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor. Applied Genet., 95: 714-722.
Direct Link  |  

Tanksley, S.D. and R.A. Jones, 1986. Application of alcohol bedydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hort. Sci., 16: 179-181.

Thanh, V.C., N.N. Tran, H. Yutaka and V.T. Nguen, 2003. Antenna protein diversity of prawns (Macrobrachium) in the Mekong Delta. Biosphere Conserv., 5: 11-17.

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