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Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 11 | Page No.: 973-983
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Research Article

Biodegradation of Trichloroethylene (TCE) in the Presence of Phenolic Compound

Muhammad Ferhan

ABSTRACT

Experimental bioreactors operated as closed recirculation systems were inoculated with aerobic bacterial cultures that utilized tryptone–yeast extract as carbon and energy sources. These were inoculated with the bacterial culture, which degraded trichloroethylene (TCE) and was observed after 5 days of incubation. Each bioreactor consisted of an expanded bed column through which the liquid phase was recirculated. TCE degradation was also observed with the metabolism of aromatic hydrocarbons established for indigenous microbial population in soil and ground water, in which TCE removal has been shown to be stimulated by the addition of phenol. So co-metabolism occurred when a non-specific enzyme or co-factor was used to transform the growth supporting carbon source, also capable of degrading non-growth supporting compounds. Gas chromatography was use to monitor TCE and their metabolites which compare to run their standards and to check their retention time (tr) values. The retention time (tr) values of phenol, catechol, TCA, TCE were 7.22, 8.82, 8.55 and 2.25.
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