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Genotypic Differences of Two Wheat Cultivars for the Enzymes Activity, Amino Acids and Protein Profile under Fe Deficiency

Amal A. Mohamed , F.K. El- Baz and R.K.H.M. Khalifa

A pot experiment was carried out at the National Research Centre, Dokki, Cairo-Egypt using water culture technique. The possibility of using some biochemical and molecular markers as a tool for the diagnosis of iron deficiency was studied in two bread wheat cultivars (Triticum aestivum L.) cv, Sids I and Giza 164 which were different in their response to iron deficiency. When iron was supplied at two levels (0 and 80 µM Fe-EDTA) the results indicated that, at 15 days old, the concentration of active iron (ferrous form) in leaves were closely correlated with visual iron deficiency symptoms of both cultivars. Generally chlorophylls concentration in leaves of both cultivars were decreased in deficient plant compared to sufficient one. On the other hand a distinct differences in the amount and types of amino acids was found in both cultivars whereas the total amount of amino acids of chlorotic leaves were higher than non chlorotic one in Giza 164 cultivar. Activities of peroxidase and catalase enzymes in leaves were also determined. Fe deficiency treatment resulted in decrease enzyme activities. Furthermore the profile of isoperoxidase and proteins were modified in leaves and roots of both cultivars during stress condition as evident from the electrogram.

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Amal A. Mohamed , F.K. El- Baz and R.K.H.M. Khalifa , 2003. Genotypic Differences of Two Wheat Cultivars for the Enzymes Activity, Amino Acids and Protein Profile under Fe Deficiency. Journal of Biological Sciences, 3: 864-874.

DOI: 10.3923/jbs.2003.864.874


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