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In vitro Rapid Regeneration of Plantlets from Leaf Explant of Native-olive (Elaeocarpus robustus Roxb.)

M.M. Rahman, M.N. Amin , M.A.K. Azad , F. Begum and M.Z. Karim

Leaf segments from field grown mature plants of Elaeocarpus robustus Roxb. were used to initiate cultures for inducing callus and subsequent differentiation of shoot buds. Callus production was obtained within seven weeks and adventitious shoot formation was observed after five weeks of incubation on ½ MS medium supplemented with 0.5 mg l-1 BA + 0.1 mg l-1 NAA + 15% CW. Complete plantlets were obtained upon transfer of shoot cuttings on ½ MS with 0.1-2.0 mg l-1 IBA, NAA, IAA or without any auxin. The best and healthy rooting was observed on ½ MS medium supplemented 0.2 mg l-1 IBA. In vitro regenerated plantlets were transferred to potting soil and successfully established under natural condition with about 60% survival.

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M.M. Rahman, M.N. Amin , M.A.K. Azad , F. Begum and M.Z. Karim , 2003. In vitro Rapid Regeneration of Plantlets from Leaf Explant of Native-olive (Elaeocarpus robustus Roxb.). Journal of Biological Sciences, 3: 718-725.

DOI: 10.3923/jbs.2003.718.725


Amin, M.N. and V.S. Jaiswal, 1987. Rapid clonal propagation of guava through in vitro shoot proliferation of nodal explants of mature trees. Plant Cell Tissue Organ Cult., 9: 235-245.

Amin, M.N., 1991. Callus culture and plant regeneration from an economically important tropical tree-jackfruit. Proceedings of the International Conference Genetic Engineering and Biotechnology, (ICGEB`91), Katmandu, Nepal, pp: 50-50.

Amin, M.N., M.A. Habib, M.A.K. Azad and M. Akhteruzzaman, 2001. Large scale plant regeneration in Ficus religiosa L. Through tissue culture. Proceedings of the 4th International Plant Tissue Culture Conference, Nov. 1-3, Dhaka, Bangladesh, pp: 9-9.

Bornman, C.H., 1983. Possibilities and constraints in the regeneration of tree from cotyledonary nuddles of Picea abies in vitro. Physiol. Plant, 57: 5-16.

Dong, J.Z. and S.R. Jia, 1991. High frequency plant regression from cotyledon of watermelon (Citrullus vulgaris Schrad.) Plant Cell Rep., 9: 559-562.

Dutton, P.R., 1976. Artocarpus heterophyllus Jackfruit. In: The Propagation of Tropical Fruit Trees, Garner, R.J. and S.A. Chaudhuri (Eds.). FAO, CAB, London, pp: 269-290.

Gamborg, O.L., T. Murashige, T.A. Thorpe and I.K. Vasil, 1976. Plant tissue culture media. In vitro Cell. Dev. Biol.-Plant, 12: 473-478.
CrossRef  |  Direct Link  |  

Ghani, A., 1998. Monographs Elaeocarpus serratus Linn. Asiatic. Society of Bangladesh, Bangladesh, pp: 167-168.

Hutchinson, J.F., 1981. Tissue culture propagation of fruit trees. Proceedings of the Symposium on Tissue Culture of Economically Important Plants, (TCEIP'81), Singapore, pp: 113-120.

Islam, R., M.R. Karim, M. Hossain and O.I. Joarder, 1992. Plant regeneration from leaf-derived callus in Aegle marmelos Corr. Plant Tissue Cult., 2: 55-59.
Direct Link  |  

Jahan, M.A.A. and S. Hadiuzzaman, 1996. Callus induction and plant regeneration from different explants of Solanum nigrum L. seedlings. Plant Tissue Cult., 6: 57-62.

James, D.J., 1988. Cell and Tissue Culture Technology for the Genetic Manipulation of Temperate Fruit Trees. In: Biotechnology of Higher Plants, Russell, G.E. (Ed.). Intercept Dorset, England, UK., pp: 33-80.

Khan, M.S. and M.K. Alam, 1996. Elaeocarpus floribundus. In: Homestead Flora of Bangladesh, Amin, S.M.R. and F.U. Ahmed (Eds.). BRAC, Dhaka, Bangladesh, pp: 57.

Khatun, A. and M.T. Hossain, 2001. In vitro micropropagation of pomegranate (Punica granatum). Proceedings of the 4th International Plant Tissue Culture Conference, Nov. 1-3, Dhaka, pp: 27-27.

Kouider, M., S.S. Korban, R.M. Skirvin and H. Joung, 1985. The relationship of apple embryos and their cotyledon to maturity dormancy and the potential to form shoots in vitro. J. Am. Soc. Hort. Sci., 110: 93-96.
Direct Link  |  

Litz, R.E. and R.A. Conover, 1980. Partial organogenesis in tissue culture of Averrhoa carambols. Hort. Sci., 15: 735-735.

Litz, R.E., G.A. Moore and C. Srinivasan, 1985. In vitro systems for propagation and improvement of tropical fruits and palms. Hort. Rev., 7: 157-198.
CrossRef  |  Direct Link  |  

Niedz, R.P., S.S. Smith, K.B. Dunbar, C.T. Stephens and H.H. Murakishi, 1989. Factors influencing shoot regeneration from cotyledonary explants of Cucumis melo Plant Cell Tissue Organ Cult., 18: 313-319.
CrossRef  |  Direct Link  |  

Rahman, M.M., M.N. Amin and M.A.K. Azad, 2001. Micropropagation of a dwarf variety of native-olive (Elaeocarpus robustus Roxb.). Proceedings of the 4th International Plant Tissue Culture Conference, Nov. 1-3, Dhaka, pp: 10-10.

Rahman, M.M., P.K. Roy, M.A. Mannan and S.K. Roy, 1999. Clonal propagation of Emblica officinalis through in vitro culture. Plant Tissue Cult., 9: 17-23.

Roy, S.K., M.S. Islam and J. Sen, 1993. Clonal propagation of Elaeocarpus robustus through in vitro culture. Proceedings of the International Plant Tissue Culture Conference, Dec. 12-19, Dhaka, Bangladesh, pp: 44-44.

Samaddar, H.N., 1990. Jackfruit. In: Fruit: Tropical Fruit and Subtropical, Bose, T.K. and S.K. Mitra (Eds.). Naya Prokash, Calcutta, India, pp: 638-639.

Sen, J., M.S. Islam and S.K. Roy, 1992. Micropropagation of juvenile and adult Gmelina arborea. Plant Tissue Cult., 2: 89-95.

Thorpe, T.A., 1982. Carbohydrate Utilization and Metabolism. In: Tissue Culture in Forestry, Bonga, J.M. and D.J. Durzan (Eds.). Martinus Nijhoff, The Hague, Netherlands, pp: 325-368.

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