• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. Journal of Biological Sciences
  2. Vol 3 (6), 2003
  3. 591-595
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 6 | Page No.: 591-595
DOI: 10.3923/jbs.2003.591.595
crossmark

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Research Article

Effect of Nitrogen and Phosphate Sources on the Biosynthesis of β-Fructofuranosidase

Mirza Ahsen Baig, Kiran Shafiq, Shazia Mirza, Sikander Ali and Ikram-ul-Haq

ABSTRACT


Saccharomyces species GCA-II was used to investigate the effect of mineral constituents on the production of β -fructofuranosidase in submerged fermentation. Different organic nitrogen and phosphate sources in varied concentrations were tested for optimal production of enzyme. The results indicated that enzyme production increased from 107.42 to 168.58 U ml-1. Thus, Saccharomyces strain GCA-II gave maximal β -fructofuranosidase in submerged fermentation when urea (3.0 g l-1) as a nitrogen source and K2HPO4 (0.20 g l-1) as phosphate source was supplied in fermentation medium.
PDF References Citation

How to cite this article

Mirza Ahsen Baig, Kiran Shafiq, Shazia Mirza, Sikander Ali and Ikram-ul-Haq, 2003. Effect of Nitrogen and Phosphate Sources on the Biosynthesis of β-Fructofuranosidase. Journal of Biological Sciences, 3: 591-595.

DOI: 10.3923/jbs.2003.591.595

URL: https://scialert.net/abstract/?doi=jbs.2003.591.595

REFERENCES


  1. Ashokkumar, B., N. Kyalvizhi and P. Gunasekaran, 2001. Optimization of media for β-fructofuranosidase production by Aspergillus niger in submerged and solid state fermentation. Process Biochem., 37: 331-338.
    CrossRefDirect Link

  2. Dworschack, R.G. and L.J. Wickerham, 1960. Extracellular invertase by sucrose-fermenting yeasts. U.S. Patent No. 2953500.

  3. Egorov, S.N., I.N. Semenova and V.N. Maksimov, 2000. Mutual effect of invertase and acid phosphatase from the yeast Saccharomyces cerevisiae on their secretion into culture media. Mikrobiologiia, 69: 34-37.

  4. Gomez, S.J.R., C. Augur and G. Viniegra-Gozalez, 2000. Invertase production by Aspergillus niger in submerged and solid-state fermentation. Biotechnol. Lett., 22: 1255-1258.
    CrossRefDirect Link

  5. Haq, I., K. Shafiq, A. Sikander and M.A. Qadeer, 2002. Production of enzyme invertase by Saccharomyces cerevisiae. Indian J. Plant Sci., 1: 5-8.

  6. Pitombo, R.N.M., C. Spring, R.F. Passos, M. Tonato and M. Vitole, 1994. Effect of moisture content on invertase activity of freeze-dried Saccharomyces cerevisiae. Cytobiology, 31: 383-392.
    PubMedDirect Link

  7. Kiran, S., S. Ali and Ikram-ul-Haq, 2002. Effect of different mineral nutrients on invertase production by Saccharomyces cerevisiae GCB-K5. Biotechnology, 1: 40-44.
    CrossRefDirect Link

  8. Silveira, M.C., E.M. Oliveira, E. Carvajal and E.P. Bon, 2000. Nitrogen regulation of Saccharomyces cerevisiae invertase. Role of the URE2 gene. Applied Biochem. Biotechnol., 84-86: 247-254.
    Direct Link

  9. Sumner, J.B. and S.F. Howell, 1935. A method for determination of saccharase activity. J. Biol. Chem., 108: 51-54.
    Direct Link

  10. Tasun, K., P. Chose and K. Glien, 1970. Sugar determination of DNS method. Biotechnol. Bioeng., 12: 921-921.

Related Articles

Effect of Different Mineral Nutrients on Invertase Production by Saccharomyces cerevisiae GCB-K5

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved