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Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 4 | Page No.: 380-387
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Research Article

Effect of Fertilizer and Inoculation on Mineral Nitrogen Contents of Pot Culture Mature Soybean Seeds

Abdul Kabir Khan Achakzai and Safdar Ali Kayani

ABSTRACT

One year Pot culture experiment on Soybean in Soil + FYM (3:1) was conducted in medium textured and salt free soil of Quetta, Balochistan. Seven different treatments (T) of fertilizer were applied to both non-inoculated (non-inoc) and inoculated (inoc) set of experiment. T1 was kept control; T2 contained 23 + 60 + 30 kg NPK ha-1 and from T3 to T7 N fertilizer in the form of urea was added @ 25, 50, 75, 100 and 125 kg ha-1 along with combination of the same constant dose of P2O5 and K2O respectively. After physiological maturity, seeds were analyzed for their mineral N contents. Results showed that fertilizer treatments in general significantly, but negatively influenced the NO3-N and NH4-N, but reverse was true for NO2-N. A minimum level of NO3-N was recorded in T4 (0.065 μ M), NH4-N in T7 (0.133 g kg 1) and a maximum level of NO2-N in T5 (1.864 g kg-1). While by comparing the inoc with non-inoc treatments in particular doses of fertilizer, inoculation in general significantly and positively influenced the total mineral N content of mature soybean seeds. A maximum level of NO3-N was noted in T4 (0.122 μ M), NH4-N in T1 (0.466 g kg-1) and NO2-N in T4 (4.314 g kg‑1). However, by comparing the marginal mean values, inoc seeds comparatively produced greater amount of total mineral N content than that of non-inoc seeds. It is therefore safely concluded that low level of NO3-N followed by NH4-N in non-inoc seeds might have preferentially incorporated it into various nitrogenous organic compounds during the entire course of seed development to physiological maturity. The correlation coefficient (r) studies revealed that NO3-N and NO2-N were significantly and positively correlated with oil (0.445 and 0.489), but non-significantly correlated with protein (-0.020 and 0.041) and grain yield (0.210 and -0.206) respectively. While reverse was true in case of NH4-N association.
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