Subscribe Now Subscribe Today
Research Article

Somatic Serotyping and Pathogenicity Testing of P. multocida Strains Isolated from Layer Chickens

Muhammad Javed Arshad , Muhammad Siddique and Iftikhar Hussain
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

During the year 2000-2001, P. multocida isolated from natural outbreaks of fowl cholera were subjected to somatic serotyping for the first time in Pakistan. Serotyping was carried out by the gel-diffusion precipitin test. P. multocida serotype 1 predominated with the percentage of 47.62, followed by serotype 3(28.57%), 4(14.29%) and 12(09.52%). All the serotypes found highly virulent with 100% mortality after challenge by serotype 1 and 3, 80% by serotype 4 and 60% by serotype 12.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Muhammad Javed Arshad , Muhammad Siddique and Iftikhar Hussain , 2003. Somatic Serotyping and Pathogenicity Testing of P. multocida Strains Isolated from Layer Chickens. Journal of Biological Sciences, 3: 376-379.

DOI: 10.3923/jbs.2003.376.379


Aye, P.P., E.J. Angrick, T.Y. Morishita and B.S. Harr, 2001. Prevalence and characteristics of P. multocida in commercial turkeys. Avian Dis., 45: 182-190.
Direct Link  |  

Borgden, K.A. and R.A. Packer, 1979. Comparison of P. multocida serotyping systems. Am. J. Vet. Res., 40: 1332-1335.
Direct Link  |  

Christensen, J.P. and M. Bisgaard, 2000. Fowl cholera. Rev. Sci. Technol., 19: 626-637.

Duguid, J.P., B.P. Marmion and R.H.A. Swain, 1989. Mackie and McCartney Medical Microbiology. 13th Edn., Churchill Livingston, Edinburgh, UK., pp 257.

Hansen, L.M. and D.C. Hirsh, 1989. Serum resistance is correlated with encapsulation of avian strains of P. multocida. Vet. Microbiol., 21: 177-184.
Direct Link  |  

Heddleston, K.L., J.E. Gallagher and P.A. Rebers, 1972. Fowl cholera: Gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian Dis., 16: 925-936.
Direct Link  |  

Jurga, I. and O.B. Borkowska, 2001. Determining the serotypes of Pasteurella multocida strains isolated from poultry. 57: 193-196.

Mushin, R., 1979. Serotypes of Pasteurella multocida isolants from poultry. Avian Dis., 23: 608-615.
Direct Link  |  

OIE., 2000. Principals of Veterinary Vaccine Production. FAO and OIE, World Org., Paris.

Rhoades, K.R. and R.B. Rimler, 1990. Somatic serotypes of Pasteurella multocida strains isolated from avian hosts (1976-1988). Avian Dis., 34: 193-195.
Direct Link  |  

Rhoades, K.R. and R.B. Rimler, 1991. Avian Pasteurellosis: Diseases of Poultry. 9th Edn., Iowa State University Press, Ames, Iowa, pp: 145-162.

Rimler, R.B., 1987. Cross-protection factors of P.multocida:passive immunity of turkeys against fowl cholera caused by different serotypes. Avian Dis., 31: 884-887.
Direct Link  |  

Rimler, R.B., R.D. Angus and M. Philips, 1989. Evaluation of the specificity of P. multocida somatic antigen-typing antisera prepared in chickens, using ribosome-lipopolysaccharide complexes as inocula. Am. J. Vet. Res., 50: 29-31.
Direct Link  |  

Snipes, K.P., G.Y. Ghazikhanian and D.C. Hirsh, 1987. Fate of P. multocida in the blood vascular system of turkey following intravenous inoculation: Comparison of an encapsulated, virulent strain with its avirulent, a capsular variant. Avian Dis., 31: 254-259.
Direct Link  |  

©  2020 Science Alert. All Rights Reserved