Subscribe Now Subscribe Today
Fulltext PDF
Research Article

Citric Acid Fermentation by Aspergillus niger NG-110 in Shake Flask

Rubina Mazhar, Sikander Ali , Ikram-ul-haq and Abdul Waheed

The present study was conducted on the optimization of cultural conditions for the production of citric acid by mutant strain of Aspergillus niger NG-110 by using shake flask technique. Different cultural conditions such as alcohol (1% methanol) and calcium chloride (2%) were optimized for enhanced citric biosynthesis. The results showed that maximum amount of anhydrous citric acid (81.21 ± 0.2 gl -1) was obtained after 168 h inoculation, with a sugar consumption of 92.20 ± 3.5 gl -1. The dry weight of mycelia was 20.40 ± 0.2 gl -1. On the basis of comparison of kinetic parameters namely the product and growth yield coefficients (Yp/s, Yp/x), volumetric rates (Qp) and specific rate constants (qp), the mutant strain of Aspergillus niger NG-110 was a faster growing organism and had the ability to hyper produce citric acid.

Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

Rubina Mazhar, Sikander Ali , Ikram-ul-haq and Abdul Waheed , 2003. Citric Acid Fermentation by Aspergillus niger NG-110 in Shake Flask. Journal of Biological Sciences, 3: 360-370.

DOI: 10.3923/jbs.2003.360.370


Ali, S., H. Ashraf and Ikram-ul-Haq, 2002. Enhancement in citrate production by alcoholic limitation. J. Biological Sci., 2: 70-72.
CrossRef  |  Direct Link  |  

Clark, D.S., 1962. Submerged citric acid fermentation of ferrocyanide treated cane molasses. Biotechnol. Bioeng., 4: 17-21.
Direct Link  |  

Fiedurek, J., B. Plute, J. Szezodrak and J. Jainroz, 1996. Relationship between citric acid and extracellular acid phosphate production by Aspergillus niger. Acta Biotechnol., 16: 207-213.
Direct Link  |  

Haq, I., S. Ali and M.A. Qadeer, 2001. Fed-batch culture studies during citric acid fermentation by Aspergillus niger GCMC-7. Biologia, 45: 32-37.

Haq, P.B. and D.A. Daud, 1995. Process of mycelial dry weight calculation for citric acid. J. Biotechnol., 9: 31-35.

Kamal, K.P., U.N. Verma, A.K. Nag and S.P. Singh, 1999. Effect of some antifoam and oxygen transfer rate on citric acid production by submerged fermentation. Asian J. Chem., 11: 1020-1022.

Marier, J.R. and M. Boulet, 1958. Direct determination of citric acid in milk with an improved pyridine-acetic anhydride method. J. Dairy Sci., 41: 1683-1692.
CrossRef  |  Direct Link  |  

Obaidi, Z.S. and R. Berry, 1979. Citric acid fermentation by submerged mould culture. Biotech. Lett., 1: 221-221.

Panda, T., S. Kundu and S.K. Majumdar, 1984. Studies on citric acid production by Aspergillus niger using treated Indian cane-molasses. Microbiol. J., 5: 61-66.

Pazouki, M., P.A. Felse, J. Sinha and T. Panda, 2000. Comparative studies on citric acid production by Aspergillus niger and Candida lipolytic using molasses and glucose. Bioprocess Eng., 22: 353-361.
Direct Link  |  

Pera, L.M. and D.A. Callieri, 1997. Influence of calcium on fungal growth, hyphal morphology and citric acid production in Aspergillus niger. Folia Microbiologica, 42: 551-556.
CrossRef  |  Direct Link  |  

Pirt, S.J., 1975. Principles of Microbes and Cultivation. Blackwell and Sons, London, UK.

Rajoka, M.I., M.N. Ahmad, R. Shahid, F. Latif and S. Pervez, 1998. Citric acid production from sugar-cane molasses by cultures of Aspergillus niger. Biologia, 44: 241-253.

Rohr, M., 1998. A century of citric acid fermentation and research. Food Technol. Biotechnol., 36: 163-171.

Shankaranand, V.S. and B.K. Lonsane, 1993. Sugar cane used as a novel substrate for production of citric acid by solid-state fermentation. World J. Microbiol. Biotechnol., 9: 377-380.

Tasun, K., P. Chose and K. Glien, 1970. Sugar determination of DNS method. Biotechnol. Bioeng., 12: 921-921.

Vergano, M.G., N. Femndez, M.A. Soria and M.S. Kerber, 1999. Influence of inoculum oreparation on citric acid production by Aspergillus niger. J. Biotechnol., 12: 655-656.

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract