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Research Article

In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties

M. Asaduzzaman , M.A. Bari , M.H. Rahman , N. Khatun , M.A. Islam and M. Rahman
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Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.

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  How to cite this article:

M. Asaduzzaman , M.A. Bari , M.H. Rahman , N. Khatun , M.A. Islam and M. Rahman , 2003. In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties . Journal of Biological Sciences, 3: 167-171.

DOI: 10.3923/jbs.2003.167.171



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