Subscribe Now Subscribe Today
Research Article

In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties

M. Asaduzzaman , M.A. Bari , M.H. Rahman , N. Khatun , M.A. Islam and M. Rahman
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Anthers of five rice varieties viz. BR-5, BR-31, BR-34, BR-37 and BR-38 were cultured for callus induction and plant regeneration. Anthers were cultured on N6, Z2 and R2 media containing the same hormonal combination 2.5mg/l NAA, 0.5mg/l Kn and 0.5mg/l 2.4-D and incubated at 25 ± 1oC in dark for callus induction. All the varieties in Z2 medium, two varieties in N6 medium and only one variety in R medium produced callus. Out of all responding varieties BR-38 produced highest percentage of callus. Calli induced in different induction media were transferred to MS medium supplemented with 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l NAA and 1.0 mg/l Kn + 1.0 mg/l NAA and incubated at 25 ± 1oC in light for plantlet regeneration. Green plantlets appeared within 15-30 days of culture and highest number of regenerated green (33.32%) and albino (11.27%) plantlets were produced in BR-37.

Related Articles in ASCI
Search in Google Scholar
View Citation
Report Citation

  How to cite this article:

M. Asaduzzaman , M.A. Bari , M.H. Rahman , N. Khatun , M.A. Islam and M. Rahman , 2003. In vitro Plant Regeneration Through Anther Culture of Five Rice Varieties . Journal of Biological Sciences, 3: 167-171.

DOI: 10.3923/jbs.2003.167.171



1:  Cao, M.Q., F. Li, T. Jiang and G.S. Li, 1995. Application of anther culture and isolated micro spore culture to vegetable crops improvement. Acta Hortic., 392: 27-38.

2:  Chen, C. and M. Lin, 1976. Induction of rice plantlets from anther culture. Bot. Bull. Acad. Sin., 17: 18-24.

3:  Chu, C.C., 1982. Haploid in Plant Improvement. In: Plant Improvement and Somatic Cell Genetics, Vasil, I.K., W.K. Scowcroft and K.J. Frey (Eds.). Academic Press, New York, pp: 129-158

4:  Chu, C.C., C.C. Wang, C.S. Sun, K.C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi, 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scient. Sin., 18: 659-668.
Direct Link  |  

5:  Datta, S.K., K. Datta and I. Potrykus, 1990. Embryogenesis and plant regeneration from micro spores of both Indical and Japonica rice (Oryza sativa). Plant Sci., 7: 83-88.

6:  Dunwell, J.M., 1986. Pollen, Ovule and Embryo Culture as Tools in Plant Breeding. In: Plant Tissue Culture and its Agricultural Application, Withers, L.A. and P.G. Alderson (Eds.). Butterworths, London, pp: 375-404

7:  Guha-Mukejee, S., 1973. Genotypic differences in vitro formation and embryoids from rice pollen. J. Exp. Bot., 24: 139-144.

8:  Iyer, R.D. and S.K. Raina, 1972. The early ontogeny of embryoids and callus from pollen and subsequent organogenesis in anther cultures of Datura metel and rice. Planta, 104: 146-156.

9:  Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Planta., 15: 473-497.
CrossRef  |  Direct Link  |  

10:  Niizeki, H. and K. Oono, 1968. Induction of haploid rice plants from another culture. Proc. Jap. Acad., 44: 554-557.

11:  Villenx, R.E., 1994. Development of new cultivars via anther culture. Hortic. Sci., 29: 1238-1240.
Direct Link  |  

12:  Chen, C.C., 1977. In vitro development of plants from micro spores of rice. In vitro, 13: 484-489.
Direct Link  |  

13:  Karim, N.H.N., A.K.M. Shahjahan, Maksuda, A. Nahar, S.A. Miah and M.Z. Haque, 1991. Improved media for callus induction from anthers of indica rice (Oryza sativa). Plant Tissue Cult., 1: 43-50.

14:  Oono, K., 1975. Production of haploid plants of rice by anther culture and their use in breeding. Bull. Nat. Inst. Agric. Sci., 260: 139-222.

©  2021 Science Alert. All Rights Reserved