Callus cultures were initiated from axillary shoots on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D, NAA, and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg l G 1 NAA and 2.0 mg l G 1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (86.00%) from axillary shoot derived callus on MS medium containing 1.0 mg l G 1 NAA and 2.5 mg l G 1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
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N.A. Siddique, M.A. Bari, N. Khatun, M. Rahman, M.H. Rahman and S. Huda, 2003. Plant Regeneration from Nodal Segments Derived Callus in Hemidesmus indicus (L.) R. Br (Anantamul) an Endangered Medicinal Plant in Bangladesh. Journal of Biological Sciences, 3: 1158-1163.