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Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 12 | Page No.: 1158-1163
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Research Article

Plant Regeneration from Nodal Segments Derived Callus in Hemidesmus indicus (L.) R. Br (Anantamul) an Endangered Medicinal Plant in Bangladesh

N.A. Siddique, M.A. Bari, N. Khatun, M. Rahman, M.H. Rahman and S. Huda

ABSTRACT

Callus cultures were initiated from axillary shoots on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D, NAA, and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg l G 1 NAA and 2.0 mg l G 1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (86.00%) from axillary shoot derived callus on MS medium containing 1.0 mg l G 1 NAA and 2.5 mg l G 1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
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