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Research Article

Clonal Propagation of Averrhoa carambola Linn. Through Nodal Culture of Mature Tree

M. Khalekuzzaman , M.F. Alam and M. Nuruzzaman
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Averrhoa carambola Linn. was micropropagated from nodal explants through axillary branching on MS medium supplemented with benzyladenine (BA) and Kinetin, Maximum numbers of shoots per explant were obtained on MS medium supplemented with 1.0 mg L-1 each of BA and Kinetin. Subculturing of regenerated shoots in MS medium having low concentration of BA (0.1 mg L-1) stimulates shoot elongation. Regenerated shoots were rooted by treating them with auxins and best root induction (70%) was observed in half-strength MS medium supplemented with 0.5 mg L-1 indole-3-butyic acid (lBA). Rooted plantlets were successfully established in soil.

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  How to cite this article:

M. Khalekuzzaman , M.F. Alam and M. Nuruzzaman , 2003. Clonal Propagation of Averrhoa carambola Linn. Through Nodal Culture of Mature Tree. Journal of Biological Sciences, 3: 1153-1157.

DOI: 10.3923/jbs.2003.1153.1157



1:  Bansal, Y.K. and T. Chibarm, 2000. Micropropagation of Madhuca latifolia Macb. through nodal culture. Plant Biotechnol., 17: 17-20.
Direct Link  |  

2:  Ghose, B. and T.S. Dhua, 1990. Carambola. In: Fruits Tropical and Subtropical, Bose T.K. and S.K. Mitra (Eds.). Naya Prakash, Calcuta, India, pp: 785-794

3:  Hutchinson, J.F. and R.A. Zimmerman, 1987. Tissue culture of temperate fruit and nut trees. Hort. Rev., 9: 273-349.
CrossRef  |  

4:  Islam, R., A. Hoque, M. Khalekuzzaman and O.I. Joarder, 1997. Micropropagation of Azadirachta indica A. Juss from explants of mature plants. Plant Tissue Cult., 7: 41-46.

5:  Islam, R., M. Khalekuzzaman, A.N.K. Mamum and O.I. Joarder, 1996. Regeneration of plantlets from in vitro cultured hypocotyl explants of Averrhoa carambola L. Crop Res., 11: 111-116.

6:  Kabir, A., R. Islam, A. Hoque, M. Khalekuzzaman and O.I. Joarder, 1994. Micropropagation of Zyzyphus mauritina from nodal segments of mature trees. Bull. Seric. Res., 5: 5-8.

7:  Khalekuzzaman, M., R. Islam and O.I. Joarder, 1995. Micropropagation of Averrhoa carambola Linn. (Carambola) by tissue culture. Bangladash J. Forest Sci., 24: 1-4.

8:  Khalekuzzaman, M., R. Islam, M.A. Reza and O.I. Joarder, 1995. Regeneration of Plantlets from in vitro cultured cotyledons of Averrhoa carambola L. (Oxalidaceae). Phytomorphology, 45: 107-111.

9:  Litz, R.E. and R.A. Conover, 1980. Partial organogenesis in tissue cultures of Averrhoa carambola. Hort. Sci., 15: 735-735.

10:  Litz, R.E. and J.L. Griffis, 1989. Carambola (Averrhoa carambola L.). In: Biotechnology in Agriculture and Forestry, Bajaj, Y.P.S. (Eds.). Springer-Verlag, Berlin, Heidelberg, pp: 59-67

11:  Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRef  |  Direct Link  |  

12:  Murashige, T., 1974. Plant propagation through tissue cultures. Annu. Rev. Plant Physiol., 25: 135-166.
CrossRef  |  Direct Link  |  

13:  Rao, A.N., V.M. Sin, N. Kathagoda and J.F. Hutchinson, 1982. Cotyledon Tissue Culture of Some Tropical Fruits. In: Tissue Culture of Economically Important Plants, Rao, A.N. (Ed.). Costed and Anbs, Singapore

14:  Roy, S.K. and M. Asaduzzaman, 1993. Clonal Propagation of Azadirachta Indica by in vitro Culture. Oxford and IBH, New Delhi, India

15:  Singh, R., 1985. Fruits. 4th Edn., National Book Trust, India, pp: 213

16:  Tidbury, G.E., 1976. Averrhoa spp. -Carambola and Bilimbi. In: The Propagation of Tropical Fruit Trees, Garner, R.J. and S.A. Chaudhary (Eds.). FAO., CAB, London, pp: 291-310

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