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Research Article

Callus Induction and Regeneration from Nodal Segment of Potato Cultivar Diamant

N. Khatun , M.A. Bari , R. Islam , S. Huda , N.A.Siddique , M.H. Rahman and M.U. Mollah
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The nodal segments of Diamant cultivar of potato from in vitro grown plantlets were cultured for callus induction and regeneration on MS semisolid medium supplemented with different concentrations of 2,4-D, NAA, BAP alone and NAA with BAP. Highest 90.00% of callus formation was observed in MS+2.5 mgl G 12,4-D. The second highest 83.33% callus induction was recorded in MS+5.0 mgl G 1 BAP. Maximum percentages (70.00%) of calli-induced shoots were observed in MS medium fortified with 5.0 mgl G 1 BAP+0.1 mgl G 1IBA. The regenerated shoots were rooted on MS and 2 MS medium containing different concentrations of IBA and maximum rooting response was achieved in 2 MS +1.0 mgl G 1IBA. Regenerated plants were successfully established in soil after acclimatization.

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  How to cite this article:

N. Khatun , M.A. Bari , R. Islam , S. Huda , N.A.Siddique , M.H. Rahman and M.U. Mollah , 2003. Callus Induction and Regeneration from Nodal Segment of Potato Cultivar Diamant. Journal of Biological Sciences, 3: 1101-1106.

DOI: 10.3923/jbs.2003.1101.1106



1:  BBS, 1999. Statistical Pocketbook of Bangladesh. Bangladesh Bureau of Statistic, Dhaka, Bangladesh, pp: 466

2:  Chee, P.P., 1990. High frequency of somatic embryogenesis and recovery of fertile cucumber plants. Hortic. Sci., 25: 792-793.

3:  De, K.K., 1992. An Introduction of Plant Tissue Culture. New Central Book Agency, Calcutta, India, pp: 167-168

4:  Evens, D.A., W.R. Sharp and C.E. Flick, 1981. Growth and BEHAVIOr of Cell Culture Embryogenesis and Organogenesis. In: Plant Tissue Culture Methods and Applications in Agriculture, Thorpe, T.A. (Ed.). Academic Press, New York, pp: 45-113

5:  Ilangantileke, S.G., M.S. Kadir, M. Hossain, A.E. Hossain, U. Jayasinghe and A.A. Mahmood, 2001. Toward alleviating poverty of rural potato farmers by strengthening the potato seed system in Bangladesh a rapid rural appraisal. CIP Program Report, pp: 259-264.

6:  Islam, R. and S. Riazuddin, 1993. Shoot organogenesis in Chiekpea (Cicer arietinum L.) from callus cultures of hypocatyle explants. J. Biol. Sci., 1: 1-5.

7:  Jaiswal, V.S. and P. Naryan, 1985. Regeneration of plantlets from the callus of stem segments of adult plants of Fucus religoisa L. Plant Cell Rep., 4: 256-258.

8:  Jones, R.L., 1994. Gibberellins their physiological role. Agric. Revue Plant Physio., 24: 571-598.

9:  Kamat, M.G. and P.S. Rao, 1987. Vegetative multiplication of egg plants (Solanum melongana) using tissue culture techniques. Plants Sci. Lett., 13: 57-65.

10:  Karth, K.K., P. Pahl, N.L. Leung and L.A. Morginski, 1981. Plant regenerationfrom meristems of grain legumes soybean cowpea peanut chickpea and bean. Can. J. Bot., 59: 1671-1679.

11:  Malamug, J.J.F., H. Inden and T. Asahira, 1991. Plantleta regeneration and propagation from ginger callus. Scientia. Hort., 48: 89-97.

12:  Mamum, A.N.K., R. Islam. M.A. Reza and O.I. Joardar, 1996. In vitro differentiation of plantlet of tissue culture if Sumanea saman. Plant Tissue Cult., 6: 1-5.

13:  Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
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14:  Nabors, M.W. and T.A. Dykes, 1985. Tissue Culture of Cereal Cultivars with Increased Salt Drought and Acid Tolerance. IRRI., Los Banos, Philippines, pp: 121-138

15:  Rao, B.G. and V.L. Chopra, 1989. Regeneration of apical meristem stem nodes and cotyledons of Chickpea. Ind. J. Pulses Res., 2: 20-24.

16:  World Book, 2000. Potato. World Book International, UK

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