Subscribe Now Subscribe Today
Abstract
Fulltext PDF
References
Research Article
 
Effect of 2, 4-D and BAP on in vitro Regeneration of Garlic



M. A. Haque , U.K. Nath , Q.N. Ahmad and S. Alam
 
ABSTRACT

Vigorously growing root tips, basal discs and leaf discs of three garlic strains (G-101, G- 102, G-103) were used as explants. The explants were collected from cloves germinated in a basal medium. Different combinations and concentrations of growth regulators like 2, 4-D and BAP were used in MS medium to observe the callus induction, proliferation, organogenesis and to produce regenerate plantlets under in vitro condition. The highest callusing was recorded at the highest concentration of 2, 4-D (2.0 mg l-1) + BAP (1.0 mg l-1) in MS medium for G-103 (80%). The other two strains (G-101, G-102) showed 70 and 75% callusing ability respectively. Highest performance in the proliferation of calli were found at MS+2,4-D (2.0 mg l-1) +BAP (0.5 mg l-1) in G-103 (90%). Maximum number of regenerated plantlets were obtained at MS + NAA (2.0 mg l-1) + BAP (1.0 mg l-1) in all the strains (35%). Plantlets thus, obtained were successfully transferred to the potted soil and subsequently to the field. The survival rate was 40-60% in all the cases.

Services
Related Articles in ASCI
Similar Articles in this Journal
Search in Google Scholar
View Citation
Report Citation

 
  How to cite this article:

M. A. Haque , U.K. Nath , Q.N. Ahmad and S. Alam , 2002. Effect of 2, 4-D and BAP on in vitro Regeneration of Garlic . Journal of Biological Sciences, 2: 771-774.

DOI: 10.3923/jbs.2002.771.774

URL: https://scialert.net/abstract/?doi=jbs.2002.771.774

REFERENCES
Anonymous, 1998. Production Year-Book. Food and Agricultural Organization of United Nations, Rome, pp: 137.

Anonymous, 1999. Bangladesh Bureau of Statistics. Govt. Peoples Republic of Bangladesh, Bangladesh, pp: 125.

Bose, T.K. and M.G. Som, 1990. Vegetable Crops in India. Naya Prokash, Calcutta, India, pp: 583-597.

Conci, V.C., D.N. Morconi and S.F. Nome, 1987. In vitro plantlet regeneration from callus in garlic. Plant Physiol., 83: 77-78.

Haque, M.S., T. Wada and K. Hattori, 1997. High frequency shoot regeneration and plantlet formation from root tip of garlic. Plant Cell Tissue Organ Cult., 50: 83-89.
CrossRef  |  Direct Link  |  

Jang, Y., Y. Oh, L. Choi, Y. Song, J. Park and Y.S. Jang, 2000. Effect of the concentration and treatment period of colchicine on polyploid formation of garlic. J. Korean Soc. Sci., 41: 157-160.

Katyal, S.L. and K.L. Chandha, 1996. Vegetable Growing in India. Mohan Priminani Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India, pp: 121-125.

Kodou, R., Y. Fujime, Y. Komastsu, N. Fukoda and K. Amimoto, 1995. Effects of plant growth regulators and cold storage of bulb on callus formation of garlic. Tec. Bull. Kagawa Univ., 47: 99-106.

Ma, Y., H. Wang, C. Zhang and Y. Kang, 1994. High rate of virus-free plantlet regeneration via garlic scape-tip culture. Plant Cell Rep., 14: 65-68.
CrossRef  |  

Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Planta., 15: 473-497.
CrossRef  |  Direct Link  |  

Seabrook, J.E.A., 1994. In vitro propagation and bulb formation in garlic. Can. J. Gen. Bulb, 74: 155-158.

Suh, S.K. and H.G. Park, 1995. Plant regeneration from the culture of garlic root explants. J. Korean Soc. Hortic. Sci., 36: 31-37.

Tapia, M., 1996. Effect of light on callus induction. Agro Cienecia, 12: 149-154.

Zhang, C.H., S.K. Shen and M. Wang, 1999. Observations of chromosome pairing behaviour and karyotype analysis in five garlic var. Acta Hortic. Sin., 26: 268-270.

Zheng, H.R., M. Shen, W. Zhong, Z. Zhang, Y. Zhou and Z.O. Zhang, 1998. Induction of calli from leaf explants. Acta Agric. Shanghai, 14: 33-38.

©  2019 Science Alert. All Rights Reserved
Fulltext PDF References Abstract