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Research Article

Enhancement in Citrate Production by Alcoholic Limitation

Sikander Ali , Hamad Ashraf and Ikram-ul-Haq
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Citric acid fermentation by Aspergillus niger GCB-47 was carried out in a 15L stainless stirred fermentor. Among the alcohols tested as stimulating agents, 1.0% methanol was found to give maximum amount of anhydrous citric acid (76.02 g/l), 24 hours after inoculation. This yield of citric acid is 1.76 fold higher than the control. The sugar consumed and %age of citric acid on the basis of sugar used were 108 g/l and 70.39 %, respectively. The mycelia were small round pellets having dry cell mass 14.5 g/l. Addition of ethanol, however, did not found to enhance citric acid production, significantly.

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  How to cite this article:

Sikander Ali , Hamad Ashraf and Ikram-ul-Haq , 2002. Enhancement in Citrate Production by Alcoholic Limitation. Journal of Biological Sciences, 2: 70-72.

DOI: 10.3923/jbs.2002.70.72


1:  Hang, Y.D. and E.E. Woodams, 1986. Utilization of grape pomace for citric acid production by solid state fermentation. Am. J. Enol. Vitric., 37: 141-142.
Direct Link  |  

2:  Haq, I., M.H. Ashraf, S. Malik and M.A. Qadeer, 1995. Production of citric acid of Aspergillus niger by solid state fermentation. Biologia, 41: 1-10.

3:  Majolli, M.V.I. and S.N. Agirre, 1999. Effect of trace metals on the cell morphology, enzymic activity and citric acid production in a strain of Aspergillus wentii. Rev. Argent Microbiol., 31: 65-71.
Direct Link  |  

4:  Maria, K. and L. Wladyslaw, 1989. Effects of medium purity on submerged citric acid fermentation yield. Acta Aliment., 15: 97-105.

5:  Moreira, M.T., A. Sanroman, G. Feijoo and J.M. Lema, 1996. Control of pellet morphology of filamentous fungi in fluidized bed bioreactors by means of a pulsing flow: Application to Aspergillus niger and Phanerochaete chrysosporium. Enzyme Microb. Technol., 19: 261-266.
CrossRef  |  

6:  Pazouki, M., P.A. Felse, J. Sinha and T. Panda, 2000. Comparative studies on citric acid production by Aspergillus niger and Candida lipolytic using molasses and glucose. Bioprocess Eng., 22: 353-361.
Direct Link  |  

7:  Roukas, T. and P. Kotzekidou, 1987. Influence of some trace metals and stimulants on citric acid production from brewery waste by Aspergillus niger. Enzyme Microb. Technol., 9: 291-294.
CrossRef  |  

8:  Saha, M.L., Y. Sakai and F. Takahashi, 1999. Citric acid fermentation by magnetic drum contactor: Use of methanol and ethanol for higher production. J. Biosci. Bioeng., 87: 369-394.
CrossRef  |  

9:  Sanjay, K. and P. Sharma, 1994. A highly performance fermentation process for production of citric acid from sugarcane molasses. J. Microbiol., 23: 211-217.

10:  Srivasta, K. and S. Kamal, 1979. Citric acid fermentation by Aspergillus niger. Indian J. Microbiol., 19: 145-149.

11:  Tasun, K., P. Chose and K. Glien, 1970. Sugar determination of DNS method. Biotechnol. Bioeng., 12: 921-921.

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