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Research Article

Antimicrobial Activity of Enterococcus faecalis Against Selected Isolates and Some Pathogenic Microorganisms

Mushtaq Ahmad , David G. Samith and S. Mahboob
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Ten isolates of Enterococcus faecalis were tested for antimicrobial activity against closely related and less closely related pathogenic gram-positive and gram-negative indicator strains. Using the agar spot test Enterococcus faecalis MI2, MI6, MI126 and MI128 showed strong activity against selected enterococcal isolates. E. faecalis MI6 and MI128 showed strong antimicrobial activity against Listeria innocua NCTC. 11288 but enterococcal isolates did not show activity against selected pathogenic strains of some other species. A clear inhibition zone around the spotted test culture observed antimicrobial activity of E. faecalis isolates. The diameters of zones of inhibition ranged from 2-8 mm.

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  How to cite this article:

Mushtaq Ahmad , David G. Samith and S. Mahboob , 2002. Antimicrobial Activity of Enterococcus faecalis Against Selected Isolates and Some Pathogenic Microorganisms. Journal of Biological Sciences, 2: 343-345.

DOI: 10.3923/jbs.2002.343.345



1:  Arihara, A., S. Ogihara, J. Sakata, M. Itoh and Y. Kondo, 1991. Antimicrobial activity of Enterococcus faecalis against Listeria monocytogenes. Lett. Applied Microbiol., 13: 190-192.

2:  Daba, H., J.F. Pandian, R.E. Gooselin, J.S. Huang and C. Lacroix, 1991. Detection and activity of bacteriocin produced by Leuconostoc mesenteroides. Applied Environ. Microbiol., 57: 350-355.

3:  Fleming, H.P., J.L. Etchells and R.N. Costilow, 1975. Microbial inhibition by an isolate of Pediococcus from cucumber brines. Applied Microbiol., 30: 1040-1042.

4:  Garvier, K.I. and P.M. Muriana, 1993. Detection, identification and characterization of bacteriocin producing LAB from retail food products. Int. J. Food Microbiol., 19: 241-258.
PubMed  |  

5:  Jack, R.W., J.R. Tagg and B. Ray, 1995. Bacteriocins of gram-positive bacteria. Microbiol. Rev., 59: 171-200.
Direct Link  |  

6:  Kaiser, A.L. and T.J. Montville, 1993. The influence of pH and growth rate on production of the bacteriocin, bavaricin MN, in batch and continuous fermentations. J. Applied Bacteriol., 75: 536-540.

7:  Kato, T., T. Matsuda, Y. Yoneyama, H. Kato and R. Nakamura, 1993. Isolation of Enterococcus faecium with antibacterial activity and characterization of its bacteriocins. Biosci. Biotechnol. Biochem., 57: 551-556.

8:  Koniskey, J., 1982. Colicins and other bacteriocins with established modes of action. Ann. Rev. Microbiol., 36: 125-144.

9:  Laukova, A., M. Marekova and P. Javorsky, 1993. Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231. Lett. Applied Microbiol., 16: 257-260.
CrossRef  |  Direct Link  |  

10:  Lewus, C.B., A. Kaiser and T.J. Montville, 1991. Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Applied Environ. Microbiol., 57: 1683-1688.

11:  Lopez-Lara, I., A. Galvez, M. Martinez-Bueno, M. Maqueda and E. Valdivie, 1991. Purification, characterization and biological effects of a second bacteriocin from Enterococcus faecalis spp. liquefaciens S-48 and its mutant strain B-48-28. Can. J. Microbiol., 37: 769-774.

12:  Mathieu, F., I.S. Suwandhi, N. Rekhif, J.B. Milliere and G. Lefebvre, 1993. Mesenterocin 52, a bacteriocin produced by Leuconostoc mesenteroides ssp. mesenteroides FR 52. J. Applied Bacteriol., 74: 372-379.
CrossRef  |  Direct Link  |  

13:  McKay, A.M., 1990. Antimicrobial activity of E. faecium against Listeria spp. Lett. Applied Microbiol., 11: 15-17.

14:  Olasupo, N.A., U. Schillinger, C.A.P. Franz and W.H. Holzapfe, 1994. Bacteriocin production by E. faecium NA01 from ~Wara-a fermented skimmed cow milk product from West Africa. Lett. Applied Microbiol., 19: 438-441.

15:  Piard, J.C., P.M. Muriana, M.J. Desmazeaud and T.R. Klaenhammer, 1992. Purification and partial characterization of lacticin 481 a lanthionine-containing bacteriocin produced by Lactococcus lactis sub. sp. Lactis CNRZ481. Applied Environ. Microbiol., 58: 279-284.
PubMed  |  

16:  Siragusa, G.R., 1992. Production of bacteriocin inhibitory to Listeria species by Enterococcus hirae. Applied Environ. Microbiol., 58: 3508-3513.

17:  Tagg, J.R., A.S. Dajani and L.W. Wannamaker, 1976. Bacteriocins of gram-positive bacteria. Bacteriol. Rev., 40: 722-756.
PubMed  |  Direct Link  |  

18:  Villani, F., G. Salzano, E. Sorrentino, O. Pepe, P. Marino and S. Coppola, 1993. Enterocin 226NWC, a bacteriocin produced by Enterococcus faecalis 226, active against Listeria monocytogenes. J. Applied Bacteriol., 74: 380-387.

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