The crude extract from dried Melia azadirachta seeds was prepared and it was noted that the seed contains sufficient amount of phospholipase A activity. Optimal activity of Melia azadirachta seeds was found at pH 6.0 and at temperature 35°C. The stability of enzyme was noted over the pH range 5-8. More than 30% activity of phospholipase A remained active up to 60 minutes at 60°C. The enzyme activity was markedly increased in the presence of calcium, manganese and zinc but it was decreased by the addition of EDTA.
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M. Umar Dahot, A.B.Ghanghro and M. Yousuf Khan, 2001. Studies on Phospholipase A from Melia azadirachta (Neem) Seeds Extract. Journal of Biological Sciences, 1: 788-790.