The 
          Receptor for Bacillus Thuringiensis Cry 1Ac Delta-endotoxin in the Brush 
          Border Membrane of the Lepidopteran Helicoverpa armigera is Aminopeptidase 
          N

, Kausar Malik; , Tariq Mahmood; , S. Riazuddin

Research Article

**The Receptor for Bacillus Thuringiensis Cry 1Ac Delta-endotoxin in the Brush Border Membrane of the Lepidopteran Helicoverpa armigera is Aminopeptidase N**

Kausar Malik, Tariq Mahmood and S. Riazuddin

ABSTRACT

120 Kda and 70 Kda protein in the larval midgut membrane of the lepidopteran Helicoverpa armigera identified as putative receptor for Bt Cry 1Ac delta-endotoxin .Receptor proteins have been purified by a combination of gel-filtration and anion exchange chromatography .In ligand-blotting experiment ,the purified protein has binding capacity with Cry 1Ac and Cry 1Ab but not to Cry 2A .N-terminal sequence obtained from the protein show no homology to already existing sequences .When assayed for amino-peptidase and alkaline phosphatase activities , purified receptors preparations were enriched 2.5 fold in amino-peptidase activity compare to Helicoverpa-armigera brush border membrane vesicles . The 70 Kda protein seems to be the part of 120 Kda receptor protein.

PDF References Citation

How to cite this article

Kausar Malik, Tariq Mahmood and S. Riazuddin, 2001. The Receptor for Bacillus Thuringiensis Cry 1Ac Delta-endotoxin in the Brush Border Membrane of the Lepidopteran Helicoverpa armigera is Aminopeptidase N. Journal of Biological Sciences, 1: 782-784.

DOI: 10.3923/jbs.2001.782.784

URL: https://scialert.net/abstract/?doi=jbs.2001.782.784

REFERENCES

Bauer, L.S. and H.S. Pankratz, 1992. Ultrastructural effects of Bacillus thuringiensis Var. San diego on midgut cells of the cotton wood leafbeetle. J. Invertebr. Pathol., 60: 15-25.
Wolfersberger, M.G., 1990. The toxicity of two Bacillus thuringiensis-endotoxin to gypsy moth larvae is inversely related to the affinity of binding sites on midgut brush border membrane for the toxins. Experientia, 46: 475-477.
Lowry, O.H., N.R. Roberts, M.L. Wu, W.S. Hixon and E.J. Crawford, 1954. The quantitative histochemistry of brain. II. Enzyme measurements. J. Biol. Chem., 207: 19-37.
PubMed
Wolfersburger, M.G., 1984. Enzymology of plasma membranes of insects intestinal cells. Am. Zool., 24: 187-197.
Luo, K., D. Banks and M.J. Adang, 1999. Toxicity, Binding and permeability analysis of four Bacillus thuringiensis Cry1. endotoxins using brush border membrane vesicles of Spodoptera exigua and Spodoptera frugiperda. Applied Environ. Microb Bio., 65: 457-464.
Nakanishi, K., K.Y. Aoi, N. Shimada, T. Kadotani and R. Sato, 1999. Bacillus thuringiensis insecticidal Cry 1Aa toxin binds to a highly conserved region of aminopeptidase N in the host insect leading to its evolutionary success. Biochim. Biophys. Acta, 1432: 57-63.
Percy, J. and P.G. Fast, 1983. Bacillus thuringiensis crystal toxin: Ultrastructural studies of its effects on silkworm midgut cells. J. Invertebr. Pathol., 41: 86-98.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
CrossRef PubMed Direct Link

Journal of Biological Sciences