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Journal of Biological Sciences

Year: 2001 | Volume: 1 | Issue: 11 | Page No.: 1078-1080
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Research Article

Screening of Exotic Pepper Lines Against Local Isolate of Chili Veinal Mottle Potyviru

Shah, H. and S. Khalid

ABSTRACT

To identify source of resistance against the most important chili virus viz. chili veinal mottle virus (CVMV), eleven exotic chili lines (CV-1, CV-2, CV-3, CV-5, CV-6, CV-7, CV-8, CV-10, CV-11, CV-12, & CV-21) were screened in controlled conditions. The isolated virus was collected from Sindh province and maintained on Nicotiana tabacum cv. Samsun by successive mechanical inoculations confirmed by double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) at each step. The seedlings were raised in sterilized soil mixture of peat, clay and sand. Plants were mechanically inoculated (1:4 w/v) at 3-4 leaf stage and the new shoots were tested through DAS-ELISA two weeks post inoculation. The ELISA negative plants were decapitated and the new sprouting were re-inoculated and ELISA was performed two weeks after inoculation. Six lines (CV-1, CV-2, CV-3, CV-7, CV-11 & CV-12) showed no symptoms and were ELISA negative, while four lines (CV-5, CV-6, CV-10, & CV-21) displayed variable reaction resulting to 5, 13, 16 and 22% infection respectively while the susceptible check (CV-8) showed severe mottling with stunted growth (100 % infection) that gave high virus titer (>2). So, asymptomatic and ELISA negative lines showed
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