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Journal of Biological Sciences

Year: 2001 | Volume: 1 | Issue: 11 | Page No.: 1063-1065
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ABSTRACT

Renal calculi collected from adult male/female patients of Multan, Pakistan were analyzed for major constituents, free amino acids and metallic contents. Composition of calculi (whole powder) was determined by chemical method using stone analysis kit. Thirteen distinct types of renal calculi were identified. Most of the calculi were compound in nature, however, some simple calculi containing calcium oxalate only were also found. Two dimensional descending paper chromatography was used to identify free amino acids in some prominent classes of renal calculi. Arginine, aspartinine, glycine, leucine, isoleucine and tryptophan were found in nearly all the calculi whereas glutamic acid, histidine, hydroxyproline, phenylalanine, threonine and tryosine occurred randomly. Analysis of major (Na, K, Ca and Mg) and trace (Fe, Zn, Cu, Ni, Pb and Mn) elements in the calculi was carried out by flame atomic absorption spectrometry. Elements such as Na, K, Ca, Mg, Fe and Zn have shown their presence in nearly all the calculi however Pb, Ni, Mn and Cu levels were below the limit of detection. This study has revealed useful information about the chemical nature of renal calculi from Multan region. It will be helpful in adopting preventive strategies to minimize stone formation and their reoccurrence.
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REFERENCES

  1. Afzal, M., M. Iqbal, H. Ahmed and J. Ahmed, 1992. Spectroscopic studies of urinary calculi in the solid state. J. Chem. Soc. Pak., 14: 171-171.
    Direct Link
  2. Contarow, A. and B. Schepartz, 1967. A Biochemistry. 4th Edn., W.B. Saunders Company, UK.
  3. Fiabance, A.M. and D.R. Williams, 1977. The Principles of Bio-inorganic Chemistry. The Chemical Society, London.
  4. Guyton, A.C. and J.E. Hall, 1996. A Textbook of Medical Physiology. 9th Edn., W.B. Saunders Co., USA.
  5. Harding, A.J., H. Rains and D. Ritchie, 1984. A Baily and Loves Short Practice of Surgery. 9th Edn., H.K. Lewis and Co. Ltd., London.
  6. Khan, F.A. and J.H. Khan, 1990. Stone survey of punjab hospitals. Pak. Postgrad. Med. J., 1: 7-7.
  7. Khaskhali, M.H., F.U. Khand and M.I. Bhanger, 1993. Pattern of free amino acids in four chemically different types of human uroliths. J. Chem. Soc. Pak., 15: 200-200.
  8. Neuman, W.F. and M.W. Neuman, 1958. The Chemical Dynamics of Bone Mineral. University of Chicago Press, Chicago, USA.
  9. Oser, B.L., 1965. A Hawks Physiological Chemistry. 14th Edn., McGraw Hill Publishing Company, New York, USA.
  10. Pak, C.Y.C. and B. Ruskin, 1970. Calcification of collagen by urine in vitro: Dependence on the degree of saturation of urine with respect to brushite. J. Clin. Invest., 49: 2353-2361.
    Direct Link
  11. Rafique, M., R.A. Bhutta, A. Rauf and I.A. Chaudhry, 2000. Chemical composition of upper renal tract calculi in Multan. J. Pak. Med. Assoc., 50: 145-151.
    Direct Link
  12. Robertson, W.G., 1973. Factors affecting the precipitation of calcium phosphate in vitro. Calcified Tissue Int., 11: 311-322.
    PubMedDirect Link
  13. Sial, S.H.J., J.H. Khan and S. Iqbal, 1995. Chemical analysis of renal calculi from D.G. Khan. Professionals Med. J., 2: 89-93.
  14. Zafar, M.H., M.I. Khan, N.M. Malik and I.H. Taseer, 1992. Prevalence and type of renal stone in Multan region. Pak. J. Med. Res., 31: 15-20.

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