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Research Article
 

Comparative Assessment of Bradyrhizobium japonicum Inoculant and Phosphorus on Growth and Yield of Soybeans (Glycine max L.) Genotypes



Nmadzuru Badeggi Ibrahim and Muhammed Mustapha Ibrahim
 
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ABSTRACT

Background and Objective: Variability in symbiotic effectiveness of soybean varieties to inoculation and phosphorus supplementation have not been fully explored. The aim of this study was to assess the comparative effects of Bradyrhizobium japonicum inoculant and single super phosphate (SSP) on growth and yield parameters of three improved soybeans genotypes. Materials and Methods: A field trial was conducted at the Teaching and Research Farm of the University of Agriculture Makurdi, Nigeria during the rainy season in 2017. The experiment was laid out in a split plot design with three soybeans genotypes constituting the main plot and the inoculant and SSP in the subplot. The inoculant and SSP were incorporated into the soil at planting. Results: Results showed that under main effects of fertilization types, inoculation with Bradyrhizobium japonicum, irrespective of variety gave the highest biomass per plant (13.61 g) and grain yield of 3.44 t ha1 while nodules and root weights were statistically higher under SSP fertilization. All varieties performed best under P fertilization across all parameters measured, although there was significant variability in P responses by individual varieties. However, the interaction of TGX 1904-6f and inoculation gave the highest grain yield of 4.62 t ha1, which indicated higher symbiotic effectiveness between this variety and Bradyrhizobium japonicum. Conclusion: Symbiotic effectiveness, P requirement and yield of soybeans varied among the different varieties and variety interaction with inoculation had the highest yield. Although P remained the major requirement for overall soybean productivity.

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  How to cite this article:

Nmadzuru Badeggi Ibrahim and Muhammed Mustapha Ibrahim, 2019. Comparative Assessment of Bradyrhizobium japonicum Inoculant and Phosphorus on Growth and Yield of Soybeans (Glycine max L.) Genotypes. Journal of Applied Sciences, 19: 782-788.

DOI: 10.3923/jas.2019.782.788

URL: https://scialert.net/abstract/?doi=jas.2019.782.788
 
Received: January 12, 2019; Accepted: March 28, 2019; Published: July 25, 2019


Copyright: © 2019. This is an open access article distributed under the terms of the creative commons attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

INTRODUCTION

Soybean (Glycine max L. Merr.) is a source of edible oil (20-25 %) and protein (42-45 %)1. According to Singh et al.2, soybean is more protein rich than any of the common vegetable or legume food sources in Africa. Among the essential nutrients needed for soybean growth, N, P and K play a crucial role in improving growth and yield3. While application of N fertilizers is not common for soybean crop especially in Africa, it is assumed that the ability of the soybean plant to fix N2 to meet its nitrogen demand and maximum crop yield is enough. The fixation of N2 from symbiosis between rhizobia and legume is a cheaper source of N and legume inoculant is usually an effective agronomic practice for ensuring adequate supply of N for legume-based crops4. The use of Bradyrhizobium inoculant to maximize biological nitrogen fixation (BNF) to meet the N requirement of soybean has been studied over time. The BNF has become an attractive and economically viable nitrogen input and an important substitute of inorganic fertilization for resource poor farmers and is environmentally friendly5.

Phosphorus deficiency is a major nutrient stress for growth and development of grain legumes6. Phosphorus is needed in relatively large proportion by legumes for growth and has been reported to promote nodule number, nodule mass, leaf area, biomass and yield in different legumes7-9. The deficiency of P supply and its availability remains a limitation on biological nitrogen fixation and symbiotic interactions as nodules are an important sink for P. Nodulation and N2 fixation are strongly influenced by P availability10. Nitrogen fixing plants have an increased requirement for P over those receiving direct nitrogen fertilization, probability due to its need for nodule development and signal transduction and to P-lipids in the large number of bacterioids11.

Because of the avoidance of environmental problems, human health and more crop production to meet the increasing need of food, integrated nutrient management by the combination of chemical and bio-fertilizers have been proposed12. Response to inoculation varies among soil types, plant species grown and season grown in different locations. Several studies have reported the response of soybeans to N fertilization and rhizobium inoculation13-16. However, estimating symbiotic effectiveness of different soybean varieties with inoculation and P fertilization and their interactions on a Savanna alfisol is lacking in reported literature. Therefore, the aim of this research was to examine the comparative effect of Bradyrhizobium japonicum inoculation and single super phosphate (SSP) on symbiotic effectiveness and productivity of soybeans varieties.

MATERIALS AND METHODS

Experimental site: The experiment was carried out as a field trial at the Teaching and Research Farm, University of Agriculture Makurdi, Nigeria, during the 2017 rainy season between August and December. Makurdi lies between latitude 7°44' N and 7°50' N and between longitude 8°30'E and 8°45'E at an average altitude of 73 m above sea level with a mean annual temperature range between 22 and 32°C and a mean annual rainfall of 1250 mm. The location falls within the southern Guinea Savannah of Nigeria.

Soil sample collection and analysis: Random soil samples were collected from the experimental site and bulked to form a composite. A sub-sample of the composite was obtained and taken to the laboratory of the Department of Soil Science, University of Agriculture Makurdi for analysis of the physical and chemical properties of the soil. Routine soil analysis was carried out using standard procedures.

Experimental layout: A field with no known history of soybean cultivation was selected for the trial. Treatments comprised of three soybeans genotype (TGX 1935-3F, TGX 1951-3F and TGX 1904-6F), Bradyrhizobium japonicum inoculant and phosphorus arranged in a split plot design and laid out in a randomized complete block design with three replications on plot sizes of 4×3 m. The main plot comprised of the soybeans genotypes while the subplot comprised of the fertilization (Control, inoculant and SSP).

Treatments application and planting: The inoculant and phosphorus were applied at planting. The inoculant was applied at 5 g kg1 of seeds using 16% gum Arabic as an adhesive as described by the two-step method17. In the first step, the weighed seeds in a container were uniformly covered with 16% (w/v) gum Arabic, then the container was closed and swirled until the seeds were uniformly coated. In the second step, the inoculant was added to the sticky seeds and the container was closed and swirled slowly until seeds were uniformly covered. The seeds were air dried to enhance adhesion and thereafter planted. The SSP was applied at the rate of 54 kg P2O5 ha1 using side placement at planting.

Planting was done at two seeds per hole at an intra-row spacing of 20 cm and inter-row spacing of 75 cm the plot was kept weed free throughout the growing season.

Data collection: At 50% flowering, five plants were sub-sampled from the border rows from each plot. They were carefully uprooted using a hand trowel and assessed for nodulation. The nodules were carefully detached and counted. The roots were detached from the shoots and the plant biomass and nodules were sub-sequently oven dried and weighed to give the nodule, root and shoot dry weights. Data was also collected on the following parameters during the growth phase of the plant: Days to first flowering, days to 50% flowering, plant height at flowering, days to first podding, days to 50% podding, days to first maturity, days to 50% maturity and plant height maturity. The mature plants were harvested and the collected pods were allowed to dry and were subsequently threshed. The seeds were dried and weighed obtain the grain weight and subsequently, yield per plot.

Statistical analysis: All data collected were subjected to two way analysis of variance (ANOVA) using the General Linear Model (GLM) and the means of measured parameters were separated using the least significant difference (LSD) at 5% level of probability. Analysis was conducted using SPSS 20.0 software (IBM Corp, Armonk, NY, USA).

RESULTS

Soil analysis: The results of the physical and chemical properties of the experimental soil determined before the establishment of the trial is shown in Table 1. The analytical values showed that the soil texture was sandy loam. The pH of the soil in water (5.9) was rated as moderately acidic. Organic carbon (3.2 g kg1), total N (0.36 g kg1) and available P (13 mg kg1) contents were rated as low. Exchangeable bases (Ca2+, Mg2+ and Na+) were all of the low class while K+ was moderate. Effective cation exchange capacity (6.73 cmol kg1) was low . The values for the available micronutrients (Zn, Cu, Mn and Fe) were classified as high.

Variety effect on productivity: The main effects of variety on nodulation, growth and yield of soybean as shown in Table 2, indicated that there were variations on these parameters across the three varieties. However, the soybean variety TGX 1951-3f gave the highest biomass yield per plot (54.8 g), biomass per plant (14.56 g) and root dry weight per plant (6.04 g), although, its grain yield (2.96 t ha1) was lower than that of TGX 1904-6f which had the highest grain yield (3.02 t ha1). The TGX 1904-6f, despite having lower growth parameters compared to the other varieties had the highest yield (3.02 t ha1).

Table 1:Experimental soil physical and chemical properties

Fertilization effect on productivity: Results presented on Table 3 showed the main effects of inoculation and SSP on growth and yield parameters of soybeans. As observed, inoculation with Bradyrhizobium japonicum, irrespective of the variety gave the highest biomass per plant (13.61 g) and grain yield of 3.44 t ha1. However, weight of nodules (1.07 g), biomass yield per plot (50.3 g) and root dry weight per plant (6.59 g) were statistically higher under SSP fertilization relative to other treatments. This showed the importance of P in root and shoots biomass production.

Variety interaction with fertilization types on productivity: Interaction effect of variety, inoculation and SSP on growth and yield of soybeans indicated that irrespective of variety, inoculation showed a consistent trend in improving plant biomass (Table 4). Under SSP fertilization, TGX 1935-3f had plant biomass per plot, root dry weight per plant and grain yield of 45.1 and 7.63 g and 3.42 t ha1, respectively. Similar parameters for TGX 1951-3f were 76.0 and 8.07 g and 3.08 t ha1, while TGX 1904-6f had values of 49.9 and 4.07 g and 2.93 t ha1, respectively. Nodulation was also increased irrespective of variety under SSP fertilization. Despite the good response of the variety TGX 1935-3f and SSP in yield (3.42 t ha1), the interaction of TGX 1904-6f and inoculation gave the highest grain yield of 4.62 t ha1, which indicated higher symbiotic effectiveness between this variety and the bacteria, Bradyrhizobium japonicum contained in the inoculant. Generally, P was shown to be of greater importance in growth and yield of soybean across the varieties.

Table 2:Variety effect on growth and yield
LSD: Least significant difference

Table 3: Effect of fertilization on growth and yield
SSP: Single super phosphate, LSD: Least significant difference

Table 4:Interaction effects of variety and fertilization on biomass and yield
P: Single super phosphate, LSD: Least significant difference

DISCUSSION

There was distinct variation in the growth and yield parameters recorded among the varieties assessed. This could be as a result of differences in genetic composition of the seeds as they were likely bred for specific traits which might affect their growth and yield attributes. Variations among yield and yield components of different varieties of soybeans have been previously reported by Haq et al.18 and Zhong19, who observed significant genotypic differences in growth and grain yield parameters. Similar variation in productivity of crop species were also reported additionally in soybean20,21, in groundnut22 and in cowpea23. Although, Zuffo et al.16 reported similarity of plant growth and productivity in two soybean cultivars with high productive potential. The varieties assessed exhibited different nodulation potentials. The use of SSP recorded the highest nodule weight per plant, biomass yield per plot as well as the root dry weight in the main effects of fertilization type. The importance of P in nodulation has been previously documented24-27. There was an observed increase in nodule number and nitrogenase activity with P application which resulted in an increase in percentage amount of N derived from bacterial fixation28,29. Similar improvement was recorded by Hayat et al.30, who reported an increase of up to 32% in amount % of N derived from bacterial fixation due to application of P. Rhizobium use phosphorus as an essential ingredient in converting atmospheric N2 to ammonium (NH4), a form useable by plants31.

Generally, genotype and SSP interaction favoured nodulation, biomass, root dry weight and grain yield. These parameters were highest in the interaction between the variety TGX 1951-3f and SSP. Phosphorus has been reported to promote early root formation and the formation of lateral, fibrous and healthy roots, which play an important role in N2 fixation, nutrient and water uptake32,33, irrespective of crop variety. Plant biomass increase due to P supply supported the report of Badar et al.34, who observed that P application significantly improved total biomass dry weight and root dry weight of groundnut, similar to the observations in this study. The importance of P on biomass production has been widely reported7,24,35.

On the main effect of fertilization, there was a significant increase in growth and yield of soybean with inoculation. This increase could be attributed to the primary aim of rhizobial inoculant which was to increase the number of desirable strains of Rhizobia in the rhizosphere to enable nitrogen fixation, thereby ultimately supplying nitrogen which was required for vegetative growth, high biomass production and improved yield36-38. Similarly, interaction effect of variety and inoculation showed that the genotype TGX 1904-6f and inoculation gave the highest grain yield indicating higher symbiotic effectiveness of this variety and the introduced bacteria to promote yield, while also indicating genotypic variation to symbiotic effectiveness among the varieties studied. Wani et al.36 stated that within grain legume species, genotypic variability affected nitrogenase activity, consequently, influencing symbiosis and productivity. Several studies have reported that application Rhizobium inoculation influenced N2 fixation and yield of legume crops such as in groundnut24,34,35, cowpea39-41 and soybeans42-45.

It was observed from this study that different varieties of soybean responded to P application in different measures. Thereby, concerns about uniform P recommendations for the same crop varieties could be raised.

CONCLUSION

Inoculation with Bradyrhizobium japonicum irrespective of the variety improved soybean productivity, although with notable variability among the varieties. Symbiotic effectiveness, phosphorus requirement and yield of soybeans varied among the different varieties studied and variety interaction with inoculation had higher plant productivity relative to P, although P remained a major requirement for soybean production.

SIGNIFICANCE STATEMENT

This study discovered that despite the general P requirement adopted for soybean cultivation, specific varieties had different P requirements and symbiotic effectiveness with Bradyrhizobium strains. This can be beneficial for farmers to avoid under or over application of growth promoting substances to specific varieties. This study will help researchers to uncover the critical areas of plant varieties growth and productivity response to inputs that many researchers were not able to explore. Thus, a new theory that symbiotic effectiveness of soybean and Bradyrhizobium japonicum will be influenced by the genetic composition of the improved crop variety which may also alter the P requirement of either the plant or bacteria or both, may be arrived at.

REFERENCES
Adewoyin, D.T.E., N.E. Mensah, O.A. Oluwafemi, D. Ogunleti, A. Adekunle and C. Kayode, 2017. Nodulation, growth and yield response of soybean [(Glycine max L. (Merril)] to inoculum (Bradyrhizobium japonicum) under phosphorus levels and compost amendment in Northern Ghana. Net J. Agric. Sci., 5: 141-150.
CrossRef  |  

Alam, N., M.J. Shim, M.W. Lee, P.G. Shin, Y.B. Yoo and T.S. Lee, 2009. Phylogenetic relationship in different commercial strains of Pleurotus nebrodensis based on ITS sequence and RAPD. Mycobiology, 37: 183-188.
CrossRef  |  PubMed  |  Direct Link  |  

Aliyu, I.A., A.A. Yusuf and R.C. Abaidoo, 2013. Response of grain legumes to rhizobial inoculation in two savanna soils of Nigeria. Afr. J. Microbiol. Res., 7: 1332-1342.
Direct Link  |  

Amba, A.A., E.B. Agbo and A. Garba, 2013. Effect of nitrogen and phosphorus fertilizers on nodulation of some selected grain legumes at Bauchi, Northern Guinea Savanna of Nigeria. Int. J. Biosci., 3: 1-7.
CrossRef  |  Direct Link  |  

Ayanlere, A.F., A.B. Mohammed, F. Dutse, M. Abdullahi and A. Muhammad-Lawal, 2012. An assessment of maize-cowpea cropping system in Oyun area of Kwara state. BEST J., 9: 39-43.

Badar, R., Z. Nisa and S. Ibrahim, 2015. Supplementation of P with rhizobial inoculants to improve growth of peanut plants. Int. J. Applied Res., 1: 19-23.
Direct Link  |  

Bekere, W. and A. Hailemariam, 2012. Influences of inoculation methods and phosphorus levels on nitrogen fixation attributes and yield of soybean (Glycine max L.) at Haru, Western Ethiopia. Am. J. Plant Nutr. Fertilizat. Technol., 2: 45-55.
CrossRef  |  Direct Link  |  

Bekere, W., T. Kebede and J. Dawud, 2013. Growth and nodulation response of soybean (Glycine max L.) to lime, Bradyrhizobium japonicum and nitrogen fertilizer in acid soil at Melko, South Western Ethiopia. Int. J. Soil Sci., 8: 25-31.
CrossRef  |  Direct Link  |  

Bhuiyan, M.M.H., M.M. Rahman, F. Afroze, G.N.C. Sutradhar and M.S.I. Bhuiyan, 2008. Effect of phosphorus, molybdenum and Rhizobium inoculation on growth and nodulation of mungbean. J. Soil Nat., 2: 25-30.
Direct Link  |  

Chaves, E., R.D.C. Leite, T.R. Silva, T.A. Viana and T.D.S. Cruz et al., 2018. Nodulation and development of soybean submitted to inoculation with Bradyrhizobium japonicum and phosphorus doses. J. Agric. Sci., 10: 321-328.
CrossRef  |  Direct Link  |  

De Freitas, A.D.S., A.F. Silva and E.V.D.S.B. Sampaio, 2012. Yield and biological nitrogen fixation of cowpea varieties in the semi-arid region of Brazil. Biomass Bioenergy, 45: 109-114.
CrossRef  |  Direct Link  |  

Fatima, Z., M. Zia and M.F. Chaudhary, 2006. Effect of Rhizobium strains and Phosphorus on growth of soybean (Glycine max) and survival of Rhizobium and P solubilization bacteria. Pak. J. Bot., 38: 459-464.
Direct Link  |  

Fatima, Z., M. Zia and M.F. Chaudhary, 2007. Interactive effect of Rhizobium strains and P on soybean yield, nitrogen fixation and soil fertility. Pak. J. Bot., 39: 255-264.
Direct Link  |  

Fontenele, A.J.P.B., M.D.F.C. Barros, R.R.A. de Vasconcelos, E.F.D.F. Silva and P.M. dos Santos, 2014. Growth of cowpea plants inoculated with Rhizobium in a saline-sodic soil after application of gypsum. Rev. Cienc. Agron., 45: 499-507.
CrossRef  |  Direct Link  |  

Graham, P.H. and C.P Vance, 2000. Nitrogen fixation in perspective: An overview of research and extension needs. Field Crops Res., 65: 93-106.
CrossRef  |  Direct Link  |  

Haq, I., I. Hussain, A.R. Khan, M. Sajid and S. Khan, 2002. Soybean genotypic response in abbottabad. Asian J. Plant Sci., 1: 418-419.
CrossRef  |  Direct Link  |  

Hayat, R., S. Ali, S.S. Ijaz, T.H. Chatha and M.T. Siddique, 2008. Estimation of N2-fixation of mung bean and mash bean through xylem ureide technique under rainfed conditions. Pak. J. Bot., 40: 723-734.
Direct Link  |  

Ibrahim, M.M., A.A. Yusuf and C.K. Daudu, 2017. Optimizing biological nitrogen fixation and yield of groundnut (Arachis hypogaea L.) in an acidic alfisol through Rhizobium inoculation, liming and fertilization. Niger. J. Sci. Res., 16: 190-196.
Direct Link  |  

Kamara, A.Y., J. Kwari, F. Ekeleme, L. Omoigui and R. Abaidoo, 2008. Effect of phosphorus application and soybean cultivar on grain and dry matter yield of subsequent maize in the tropical savannas of North-Eastern Nigeria. Afr. J. Biotechnol., 7: 2593-2599.
Direct Link  |  

Kamara, E.G., N.S. Olympio and J.Y. Asibuo, 2011. Effect of calcium and phosphorus fertilizer on the growth and yield of groundnut (Arachis hypogaea L.). Int. Res. J. Agric. Sci. Soil Sci., 1: 326-331.
Direct Link  |  

Kaschuk, G., M.A. Nogueira, M.J. de Luca and M. Hungria, 2016. Response of determinate and indeterminate soybean cultivars to basal and topdressing N fertilization compared to sole inoculation with Bradyrhizobium. Field Crops Res., 195: 21-27.
CrossRef  |  Direct Link  |  

Makinde, E.A. and O.T. Ayoola, 2010. Growth, yield and NPK uptake by maize with complementary organic and inorganic fertilizers. Afr. J. Food Agric. Nutr. Dev., 10: 2203-2217.
Direct Link  |  

Marinho, R.D.C.N., R.S.A. Nobrega, J.E. Zilli, G.R. Xavier and C.A.F. Santos et al., 2014. Field performance of new cowpea cultivars inoculated with efficient nitrogen-fixing rhizobial strains in the Brazilian semiarid. Pesqui. Agropecu. Bras., 49: 395-402.
CrossRef  |  Direct Link  |  

Moreno, G., A.J.P. Albrecht, L.P. Albrecht, C.P. Junior and L.A. Pivetta et al., 2018. Application of nitrogen fertilizer in high-demand stages of soybean and its effects on yield perform. Aust. J. Crop Sci., 12: 16-21.
Direct Link  |  

N'cho, C.O., A.A. Yusuf, J.T. Ama-Abina, M. Jemo, R.C. Abaidoo and I. Savane, 2013. Effects of commercial microbial inoculants and foliar fertilizers on soybean nodulation and yield in northern Guinea savannah of Nigeria. Int. J. Adv. Agric. Sci., 1: 66-73.
Direct Link  |  

Niu, Y.F., R.S. Chai, G.L. Jin, H. Wang, C.X. Tang and Y.S. Zhang, 2013. Responses of root architecture development to low phosphorus availability: A review. Ann. Bot., 112: 391-408.
CrossRef  |  Direct Link  |  

Nyoki, D. and P.A. Ndakidemi, 2014. Influence of Bradyrhizobium japonicum and phosphorus on micronutrient uptake in cowpea. A case study of zinc (Zn), iron (Fe), copper (Cu) and manganese (Mn). Am. J. Plant Sci., 5: 427-435.
CrossRef  |  Direct Link  |  

Okereke, G.U., C. Onichie, A. Onunkwo and E. Onyeayba, 2001. Effectiveness of foreign bradyrhizobia strains in enhancing nodulation, dry matter and seed yield of soybean (Glycine max L.) cultivars in Nigeria. Biol. Fert. Soils, 33: 3-9.
CrossRef  |  Direct Link  |  

Osunde, A.O., A. Bala, M.S. Gwam, P.A. Tsado, N. Sanginga and J.A. Okogun, 2003. Residual benefits of promiscuous soybean to maize (Zea mays L.) grown on farmers’ fields around Minna in the Southern Guinea savanna zone of Nigeria. Agric. Ecosyst. Environ., 100: 209-220.
CrossRef  |  Direct Link  |  

Saini, V.K., S.C. Bhandari and J.C. Tarafdar, 2004. Comparison of crop yield, soil microbial C, N and P, N-fixation, nodulation and mycorrhizal infection in inoculated and non-inoculated sorghum and chickpea crops. Field Crop Res., 89: 39-47.
CrossRef  |  Direct Link  |  

Sanginga, N., G. Thottappilly and K. Dashiell, 2000. Effectiveness of rhizobia nodulating recent promiscuous soyabean selections in the moist savanna of Nigeria. Soil Biol. Biochem, 32: 127-133.
CrossRef  |  Direct Link  |  

Saxena, A.K. and R.B. Rewari, 1991. Influence of phosphate and zinc on growth, nodulation and mineral composition of chickpea (Cicer arietinum L.) under salt stress. World J. Microbiol. Biotechnol., 7: 202-205.
CrossRef  |  Direct Link  |  

Sharma, U.C., M. Datta and V. Sharma, 2011. Effect of applied phosphorus on the yield and nutrient uptake by soybean cultivars on acidic hill soil. Open J. Soil Sci., 1: 45-48.
CrossRef  |  Direct Link  |  

Singh, A., A.L. Baoule, H.G. Ahmed, A.U. Dikko and U. Aliyu et al., 2011. Influence of phosphorus on the performance of cowpea (Vigna unguiculata (L.) Walp.) varieties in the Sudan savanna of Nigeria. Agric. Sci., 2: 313-317.
CrossRef  |  Direct Link  |  

Singh, A., R.J. Carsky, E.O. Lucas and K. Dashiell, 2003. Soil N balance as affected by soybean maturity class in the Guinea savanna of Nigeria. Agric. Ecosyst. Environ., 100: 231-240.
CrossRef  |  Direct Link  |  

Singleton, P.W., B.B. Bohlool and P.L. Nakao, 1992. Legume Response to Rhizobial Inoculation in the Tropics: Myths and Realities. In: Myths and Science of Soils of the Tropics, Lal, R. and P.A. Sanchez (Eds.). Soil Science and Society of America and American Society of Agronomy, USA., pp: 135-155.

Tarawali, A.R., 2014. Response of groundnut (Arachis hypogaea L.) varieties to phosphorous in three agro ecologies in Sierra Leone. Int. J. Agric. For., 4: 106-111.
Direct Link  |  

Wani, S.P., O.P. Rupela and K.K. Lee, 1995. Sustainable agriculture in the semi-arid tropics through biological nitrogen fixation in grain legumes. Plant Soil, 174: 29-49.
CrossRef  |  Direct Link  |  

Wolde-Meskela, E., J. van Heerwaarden, B. Abdulkadir, S. Kassa, I. Aliyi, T. Degefu and K.E. Giller, 2018. Additive yield response of chickpea (Cicer arietinum L.) to rhizobium inoculation and phosphorus fertilizer across smallholder farms in Ethiopia. Agric. Ecosyst. Environ., 261: 144-152.
CrossRef  |  Direct Link  |  

Woomer, P.L., 2010. Biological nitrogen fixation and grain legume enterprise: Guidelines for N2 Africa master farmers. Tropical Soil Biology and Fertility Institute of the International Centre for Tropical Agriculture, Nairobi, pp: 17.

Yakubu, H., J.D. Kwari and M.K. Sandabe, 2010. Effect of phosphorus fertilizer on nitrogen fixation by some grain legume varieties in Sudano-Sahelian Zone of North Eastern Nigeria. Niger. J. Basic Applied Sci., 18: 19-26.
CrossRef  |  Direct Link  |  

Yusuf, A.A., R.C. Abaidoo, E.N.O. Iwuafor and O.O. Olufajo, 2008. Genotype effects of cowpea and soybean on nodulation, n2-fixation and n balance in the northern guinea savanna of Nigeria. J. Agron., 7: 258-264.
CrossRef  |  Direct Link  |  

Zahran, H.H., 1999. Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiol. Mol. Biol. Rev., 63: 968-989.
PubMed  |  Direct Link  |  

Zhong, R.J., 2000. A comparative experiment on soybean cultivars in Nyigchi in Tibet. Soybean Sci., 19: 90-94.

Zuffo, A.M., F. Steiner, A. Busch and T. Zoz, 2018. Response of early soybean cultivars to nitrogen fertilization associated with Bradyrhizobium japonicum inoculation. Pesqui. Agropecu. Trop., 48: 436-446.
CrossRef  |  Direct Link  |  

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